2022
Significant cardiac IK1 inhibition and action potential prolongation induced by the combination of sildenafil and Ba2+ at low concentrations
MACHÁČEK, Martin, Olga ŠVECOVÁ a Markéta BÉBAROVÁZákladní údaje
Originální název
Significant cardiac IK1 inhibition and action potential prolongation induced by the combination of sildenafil and Ba2+ at low concentrations
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Vydání
European Muscle Conference 2022, 2022
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Organizační jednotka
Lékařská fakulta
Příznaky
Mezinárodní význam
Změněno: 11. 11. 2022 15:10, doc. MUDr. Markéta Bébarová, Ph.D.
Anotace
V originále
Sildenafil (Viagra), a commonly used vasodilatory drug, may rarely induce atrial or even ventricular fibrillation. Although changes in cardiac IK1 contribute to the pathogenesis of such arrhythmias, the effect of sildenafil on IK1 has not been studied. We investigated IK1 changes induced by various sildenafil concentrations (0.1–100 µM), and further by a combination of sildenafil and Ba2+ (a specific IK1 inhibitor, which is commonly present in human plasma in concentrations up to 1.5 µM). Experiments were performed by the whole-cell patch-clamp technique on enzymatically isolated rat ventricular cardiomyocytes. Sildenafil and Ba2+ separately caused a significant, reversible, and concentration-dependent inhibition of both inward and outward IK1 components. The combined application of sildenafil and Ba2+ (both at a very low, subclinical concentration of 0.1 µM) elicited a massive potentiation of the aforementioned effect that resulted in a significant prolongation of action potential duration (APD). To our knowledge, similar potentiation of the drug-channel interaction has never been described. The observed massive IK1 inhibition and APD prolongation induced by a combination of 0.1 µM sildenafil and Ba2+ might contribute to the genesis of sildenafil-induced arrhythmias that were rarely observed in patients treated with sildenafil.
Návaznosti
MUNI/A/1133/2021, interní kód MU |
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