Specific carbohydrate substrates affected the growth of
Bacillus cereus strain isolated from an environmental sample

a 2022

Specific carbohydrate substrates affected the growth of Bacillus cereus strain isolated from an environmental sample

VYKLICKÁ, Kateřina, Jiří KUČERA, Kateřina PAŠKOVÁ, Jana DOKULILOVA, Roman ŘEMÍNEK et. al.

Basic information

Original name

Specific carbohydrate substrates affected the growth of Bacillus cereus strain isolated from an environmental sample

Authors

VYKLICKÁ, Kateřina (203 Czech Republic), Jiří KUČERA (203 Czech Republic), Kateřina PAŠKOVÁ (203 Czech Republic), Jana DOKULILOVA, Roman ŘEMÍNEK (203 Czech Republic), Zdeněk GLATZ (203 Czech Republic) and Petra BOŘILOVÁ LINHARTOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Setkání biochemiků a molekulárních biologů, 2022, 2022

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/22:00127180

Organization unit

Faculty of Science

Keywords in English

Bacillus cereus;cultivation;carbohydrate substrates

Změněno: 30/3/2023 11:13, Mgr. Terezie Slámová

Abstract

V originále

Background Bacillus cereus is a typical food contaminant and one of the most important spoilage microorganisms in the dairy environment. B. cereus infection may lead to emetic and diarrheal diseases in humans. The optimal temperature range for B. cereus growth is reported to range from 30 °C to 40 °C. The effects of the carbohydrate substrates on the growth of B. cereus is, however, not known. This study aimed: i) to verify the optimal temperature for the growth of a particular B. cereus strain isolated from the environmental sample to be able to recommend proper storage conditions of products possibly containing this strain and ii) to investigate whether the growth of this bacterium is affected by the presence of specific carbohydrates, which might have implications for treatment of patients infected with this strain. Methods B. cereus isolated from improperly stored breast milk was statically cultured in Tryptone Soy Broth (TSB) media with or without carbohydrate substrate – glucose, fructose, galactose, lactose, sucrose, and xylitol (0.5%, w/v) – at 15 °C, 20 °C, 25 °C, 30 °C, and 37 °C (simulating physiological conditions of the human body) for 12 hours. The optical density (OD600) was measured at 600 nm to monitor cell growth. Carbohydrate substrate consumption and generation of its metabolic products at 37 °C were determined by capillary electrophoresis (CE). Results The temperature of 30 °C was optimal for the growth of the isolated strain while the growth was completely inhibited at the temperature of 15 °C. At all other temperatures, the minimum growth of B. cereus was observed after 12 hours of cultivation in substrates supplemented with lactose and galactose. The bacterial growth under these conditions was even lower than in a TSB medium without a carbohydrate substrate. On the other hand, these bacteria grew most efficiently with glucose. The CE analysis confirmed the kinetic results. Glucose consumption was 49.6 %, resulting in the production of lactate (18.4 mM), acetate (11.6 mM), and formate (1.26 mM). On the contrary, lactose and galactose consumption was only 6.47 % and 3.45 %, with acetate as the main product (10.4 mM and 12.1 mM, respectively). Conclusions To avoid the contamination of foods with the strain B. cereus used in this study, a temperature lower than 15 °C for food storage is sufficient. The optimal temperature range for B. cereus growth was confirmed, cultivation temperature 30 °C and/or eventually 37 °C are the best conditions for B. cereus growth. Moreover, we have shown that the growth of B. cereus highly depends on carbohydrate supplementation. The B. cereus growth was lowest with lactose and highest with glucose, which was also associated with the highest lactate production. These results imply that dietary supplementation with lactose might benefit patients suffering from B. cereus infection, which, however, needs to be verified in further (clinical) studies.

Links

EF17_043/0009632, research and development project

Name: CETOCOEN Excellence

LM2018121, research and development project

Name: Výzkumná infrastruktura RECETOX (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR, RECETOX RI

NU20-03-00126, research and development project

Name: Hostitelský mikrobiom ve vztahu k rozvoji Barrettova jícnu a adenokarcinomu jícnu

Investor: Ministry of Health of the CR, Host microbiome in relation to Barrett ́s esophagus and esophageal adenocarcinoma development, Subprogram 1 - standard

Citovat

VYKLICKÁ, Kateřina, Jiří KUČERA, Kateřina PAŠKOVÁ, Jana DOKULILOVA, Roman ŘEMÍNEK, Zdeněk GLATZ and Petra BOŘILOVÁ LINHARTOVÁ. Specific carbohydrate substrates affected the growth of Bacillus cereus strain isolated from an environmental sample. In Setkání biochemiků a molekulárních biologů, 2022. 2022.

@proceedings{2232459,
   author = {Vyklická, Kateřina and Kučera, Jiří and Pašková, Kateřina and Dokulilova, Jana and Řemínek, Roman and Glatz, Zdeněk and Bořilová Linhartová, Petra},
   booktitle = {Setkání biochemiků a molekulárních biologů, 2022},
   keywords = {Bacillus cereus;cultivation;carbohydrate substrates},
   language = {eng},
   title = {Specific carbohydrate substrates affected the growth of Bacillus cereus strain isolated from an environmental sample},
   year = {2022}
}

TY  - CONF
ID  - 2232459
AU  - Vyklická, Kateřina - Kučera, Jiří - Pašková, Kateřina - Dokulilova, Jana - Řemínek, Roman - Glatz, Zdeněk - Bořilová Linhartová, Petra
PY  - 2022
TI  - Specific carbohydrate substrates affected the growth of Bacillus cereus strain isolated from an environmental sample
KW  - Bacillus cereus;cultivation;carbohydrate substrates
N2  - Background Bacillus cereus is a typical food contaminant and one of the most important spoilage microorganisms in the dairy environment. B. cereus infection may lead to emetic and diarrheal diseases in humans. The optimal temperature range for B. cereus growth is reported to range from 30 °C to 40 °C. The effects of the carbohydrate substrates on the growth of B. cereus is, however, not known. This study aimed: i) to verify the optimal temperature for the growth of a particular B. cereus strain isolated from the environmental sample to be able to recommend proper storage conditions of products possibly containing this strain and ii) to investigate whether the growth of this bacterium is affected by the presence of specific carbohydrates, which might have implications for treatment of patients infected with this strain. Methods B. cereus isolated from improperly stored breast milk was statically cultured in Tryptone Soy Broth (TSB) media with or without carbohydrate substrate – glucose, fructose, galactose, lactose, sucrose, and xylitol (0.5%, w/v) – at 15 °C, 20 °C, 25 °C, 30 °C, and 37 °C (simulating physiological conditions of the human body) for 12 hours. The optical density (OD600) was measured at 600 nm to monitor cell growth. Carbohydrate substrate consumption and generation of its metabolic products at 37 °C were determined by capillary electrophoresis (CE). Results The temperature of 30 °C was optimal for the growth of the isolated strain while the growth was completely inhibited at the temperature of 15 °C. At all other temperatures, the minimum growth of B. cereus was observed after 12 hours of cultivation in substrates supplemented with lactose and galactose. The bacterial growth under these conditions was even lower than in a TSB medium without a carbohydrate substrate. On the other hand, these bacteria grew most efficiently with glucose. The CE analysis confirmed the kinetic results. Glucose consumption was 49.6 %, resulting in the production of lactate (18.4 mM), acetate (11.6 mM), and formate (1.26 mM). On the contrary, lactose and galactose consumption was only 6.47 % and 3.45 %, with acetate as the main product (10.4 mM and 12.1 mM, respectively). Conclusions To avoid the contamination of foods with the strain B. cereus used in this study, a temperature lower than 15 °C for food storage is sufficient. The optimal temperature range for B. cereus growth was confirmed, cultivation temperature 30 °C and/or eventually 37 °C are the best conditions for B. cereus growth. Moreover, we have shown that the growth of B. cereus highly depends on carbohydrate supplementation. The B. cereus growth was lowest with lactose and highest with glucose, which was also associated with the highest lactate production. These results imply that dietary supplementation with lactose might benefit patients suffering from B. cereus infection, which, however, needs to be verified in further (clinical) studies.
ER  -

VYKLICKÁ, Kateřina, Jiří KUČERA, Kateřina PAŠKOVÁ, Jana DOKULILOVA, Roman ŘEMÍNEK, Zdeněk GLATZ and Petra BOŘILOVÁ LINHARTOVÁ. Specific carbohydrate substrates affected the growth of Bacillus cereus strain isolated from an environmental sample. In \textit{Setkání biochemiků a molekulárních biologů, 2022}. 2022.

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