The association between polymorphisms in the gene for
kallikrein 4 and dental caries in primary and permanent
dentition

a 2022

The association between polymorphisms in the gene for kallikrein 4 and dental caries in primary and permanent dentition

GACHOVÁ, Daniela, Lydie IZAKOVIČOVÁ HOLLÁ, Tereza DEISSOVÁ, Břetislav LIPOVÝ, Petra BOŘILOVÁ LINHARTOVÁ et. al.

Základní údaje

Originální název

The association between polymorphisms in the gene for kallikrein 4 and dental caries in primary and permanent dentition

Autoři

GACHOVÁ, Daniela (203 Česká republika), Lydie IZAKOVIČOVÁ HOLLÁ (203 Česká republika), Tereza DEISSOVÁ (203 Česká republika), Břetislav LIPOVÝ (203 Česká republika) a Petra BOŘILOVÁ LINHARTOVÁ (203 Česká republika, garant, domácí)

Vydání

Setkání biochemiků a molekulárních biologů, 2022, 2022

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/22:00127182

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

polymorphisms;kalikrein;dental caries;dentition

Změněno: 30. 3. 2023 13:43, Mgr. Terezie Slámová

Anotace

V originále

Objectives: Dental caries is a widespread multifactorial disease, caused by the demineralization of hard dental tissues. Susceptibility to dental caries is partially genetically conditioned. This study aimed to find an association of this disease with selected single nucleotide polymorphisms (SNPs) in the gene for kallikrein 4 (KLK4), a protease secreted during amelogenesis. Methods: In this case-control study, 4 SNPs in KLK4 gene were analyzed in 150 children (105 cases and 45 caries-free controls) with primary dentition and 611 children (462 cases and 149 caries-free controls) with permanent dentition from the European Longitudinal Study of Pregnancy and Childhood (ELSPAC) cohort. SNPs were analyzed by RFLP-PCR or qPCR using TaqMan dual-labeled fluorescent probes with quencher. Results: SNP rs2242670 was associated with dental caries in both primary (p = 0.036) and permanent dentition (p = 0.04) in our cohorts. While SNP rs198968 was associated with dental caries in primary dentition (p = 0.043 in allele distribution and p = 0.019 in the recessive model), alleles with minor frequency in another position of KLK4 gene (SNPs rs2235091, and rs2978642) were considered protective for dental caries in permanent dentition (p ≤ 0.05). Moreover, specific KLK4 haplotypes were associated with dental caries in permanent dentition (p ≤ 0.05). Summary: The association between the 4 studied SNPs in KLK4 and dental caries have been proven, which supports the hypotheses that susceptibility to dental caries is partially genetically determined. Even though specific KLK4 variants are localized in intronic regions and their functional significance has not yet been determined, these SNPs should be considered in the risk assessment of dental caries in the European Caucasian population.

Návaznosti

LM2018121, projekt VaV

Název: Výzkumná infrastruktura RECETOX (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, RECETOX RI

NU20-08-00205, projekt VaV

Název: Molekulární etiopatogeneze apikální periodontitidy a odontogenních cyst

Investor: Ministerstvo zdravotnictví ČR, Molekulární etiopatogeneze apikální periodontitidy a odontogenních cyst, Podprogram 1 - standardní

NV17-30439A, projekt VaV

Název: Moderní biotechnologické a behaviorální přístupy ve výzkumu zubního kazu a strategie jeho prevence

Citovat

GACHOVÁ, Daniela, Lydie IZAKOVIČOVÁ HOLLÁ, Tereza DEISSOVÁ, Břetislav LIPOVÝ a Petra BOŘILOVÁ LINHARTOVÁ. The association between polymorphisms in the gene for kallikrein 4 and dental caries in primary and permanent dentition. In Setkání biochemiků a molekulárních biologů, 2022. 2022.

@proceedings{2232465,
   author = {Gachová, Daniela and Izakovičová Hollá, Lydie and Deissová, Tereza and Lipový, Břetislav and Bořilová Linhartová, Petra},
   booktitle = {Setkání biochemiků a molekulárních biologů, 2022},
   keywords = {polymorphisms;kalikrein;dental caries;dentition},
   language = {eng},
   title = {The association between polymorphisms in the gene for kallikrein 4 and dental caries in primary and permanent dentition},
   year = {2022}
}

TY  - CONF
ID  - 2232465
AU  - Gachová, Daniela - Izakovičová Hollá, Lydie - Deissová, Tereza - Lipový, Břetislav - Bořilová Linhartová, Petra
PY  - 2022
TI  - The association between polymorphisms in the gene for kallikrein 4 and dental caries in primary and permanent dentition
KW  - polymorphisms;kalikrein;dental caries;dentition
N2  - Objectives: Dental caries is a widespread multifactorial disease, caused by the demineralization of hard dental tissues. Susceptibility to dental caries is partially genetically conditioned. This study aimed to find an association of this disease with selected single nucleotide polymorphisms (SNPs) in the gene for kallikrein 4 (KLK4), a protease secreted during amelogenesis. Methods: In this case-control study, 4 SNPs in KLK4 gene were analyzed in 150 children (105 cases and 45 caries-free controls) with primary dentition and 611 children (462 cases and 149 caries-free controls) with permanent dentition from the European Longitudinal Study of Pregnancy and Childhood (ELSPAC) cohort. SNPs were analyzed by RFLP-PCR or qPCR using TaqMan dual-labeled fluorescent probes with quencher. Results: SNP rs2242670 was associated with dental caries in both primary (p = 0.036) and permanent dentition (p = 0.04) in our cohorts. While SNP rs198968 was associated with dental caries in primary dentition (p = 0.043 in allele distribution and p = 0.019 in the recessive model), alleles with minor frequency in another position of KLK4 gene (SNPs rs2235091, and rs2978642) were considered protective for dental caries in permanent dentition (p ≤ 0.05). Moreover, specific KLK4 haplotypes were associated with dental caries in permanent dentition (p ≤ 0.05). Summary: The association between the 4 studied SNPs in KLK4 and dental caries have been proven, which supports the hypotheses that susceptibility to dental caries is partially genetically determined. Even though specific KLK4 variants are localized in intronic regions and their functional significance has not yet been determined, these SNPs should be considered in the risk assessment of dental caries in the European Caucasian population.
ER  -

GACHOVÁ, Daniela, Lydie IZAKOVIČOVÁ HOLLÁ, Tereza DEISSOVÁ, Břetislav LIPOVÝ a Petra BOŘILOVÁ LINHARTOVÁ. The association between polymorphisms in the gene for kallikrein 4 and dental caries in primary and permanent dentition. In \textit{Setkání biochemiků a molekulárních biologů, 2022}. 2022.

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