Gold nanoparticles – from synthesis to extraction of biological
thiols and CE-LIF analysis

a 2022

Gold nanoparticles – from synthesis to extraction of biological thiols and CE-LIF analysis

DOSEDĚLOVÁ, Věra a Petr KUBÁŇ

Základní údaje

Originální název

Gold nanoparticles – from synthesis to extraction of biological thiols and CE-LIF analysis

Autoři

DOSEDĚLOVÁ, Věra a Petr KUBÁŇ

Vydání

APCE-CECE ITP-IUPAC 2022, 2022

Další údaje

Typ výsledku

Konferenční abstrakt

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

biological thiols; gold nanoparticles; capillary electrophoresis; extraction

Změněno: 24. 11. 2022 13:34, Mgr. Věra Dosedělová, Ph.D.

Anotace

V originále

Gold nanoparticles (AuNPs) exhibit several unique properties including redox activity, surface plasmon resonance, a high surface-to-volume ratio, or spontaneous formation of a stable Au-S dative bond with biological thiols. Biothiols are endogenous compounds that play a significant role in biological systems and their altered levels have been associated with disease states. However, levels of biothiols are low in non-invasive biological samples such as saliva or exhaled breath condensate (EBC). In this work, AUNP synthesis and AuNP-based extraction of biological thiols from samples followed by CE-LIF analysis are investigated. AuNPs were synthetized by reduction of tetrachloroauric acid by trisodium citrate. The increasing gold-to-citrate ratio led to synthesis of bigger particles (∼30-60 nm) but of worse particle size monodispersity. The particle size and distribution were determined by scanning electron microscopy and UV–Vis spectrophotometry. AuNPs with the size of ∼17 nm were further used for investigation of the AuNP-biothiol interaction using different AuNP stabilizing agents (i.e., citrate, Tween 20) by UV–Vis spectrophotometry. Binding of cysteamine and homocysteine on the AuNP surface caused rapid AuNP aggregation and a color change of the suspension from red to blue. On the other hand, glutathione did not cause any visible aggregation. For biothiol extraction and enrichment, EBC samples were incubated with AuNPs and different reagents were tested (i.e., dithiothreitol, thioglycolic acid) to release biothiols from AuNPs. Prior to CE-LIF analysis, biothiols were derivatized with eosin-5-maleimide and separated in the 15 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid electrolyte (pH 7.5). A further optimization of biothiol release from AuNPs to achieve better enrichment will be conducted in future research.

Návaznosti

MUNI/A/1419/2021, interní kód MU

Název: Specifický výzkum v oblasti „Life Sciences“

Investor: Masarykova univerzita, Specifický výzkum v oblasti „Life Sciences“

Citovat

DOSEDĚLOVÁ, Věra a Petr KUBÁŇ. Gold nanoparticles – from synthesis to extraction of biological thiols and CE-LIF analysis. In APCE-CECE ITP-IUPAC 2022. 2022.

@proceedings{2233720,
   author = {Dosedělová, Věra and Kubáň, Petr},
   booktitle = {APCE-CECE ITP-IUPAC 2022},
   keywords = {biological thiols; gold nanoparticles; capillary electrophoresis; extraction},
   title = {Gold nanoparticles – from synthesis to extraction of biological thiols and CE-LIF analysis},
   year = {2022}
}

TY  - CONF
ID  - 2233720
AU  - Dosedělová, Věra - Kubáň, Petr
PY  - 2022
TI  - Gold nanoparticles – from synthesis to extraction of biological thiols and CE-LIF analysis
KW  - biological thiols
KW  - gold nanoparticles
KW  - capillary electrophoresis
KW  - extraction
N2  - Gold nanoparticles (AuNPs) exhibit several unique properties including redox activity, surface plasmon resonance, a high surface-to-volume ratio, or spontaneous formation of a stable Au-S dative bond with biological thiols. Biothiols are endogenous compounds that play a significant role in biological systems and their altered levels have been associated with disease states. However, levels of biothiols are low in non-invasive biological samples such as saliva or exhaled breath condensate (EBC). In this work, AUNP synthesis and AuNP-based extraction of biological thiols from samples followed by CE-LIF analysis are investigated. AuNPs were synthetized by reduction of tetrachloroauric acid by trisodium citrate. The increasing gold-to-citrate ratio led to synthesis of bigger particles (∼30-60 nm) but of worse particle size monodispersity. The particle size and distribution were determined by scanning electron microscopy and UV–Vis spectrophotometry. AuNPs with the size of ∼17 nm were further used for investigation of the AuNP-biothiol interaction using different AuNP stabilizing agents (i.e., citrate, Tween 20) by UV–Vis spectrophotometry. Binding of cysteamine and homocysteine on the AuNP surface caused rapid AuNP aggregation and a color change of the suspension from red to blue. On the other hand, glutathione did not cause any visible aggregation. For biothiol extraction and enrichment, EBC samples were incubated with AuNPs and different reagents were tested (i.e., dithiothreitol, thioglycolic acid) to release biothiols from AuNPs. Prior to CE-LIF analysis, biothiols were derivatized with eosin-5-maleimide and separated in the 15 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid electrolyte (pH 7.5). A further optimization of biothiol release from AuNPs to achieve better enrichment will be conducted in future research.
ER  -

DOSEDĚLOVÁ, Věra a Petr KUBÁŇ. Gold nanoparticles – from synthesis to extraction of biological thiols and CE-LIF analysis. In \textit{APCE-CECE ITP-IUPAC 2022}. 2022.

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