Další formáty:
BibTeX
LaTeX
RIS
@proceedings{2234357,
author = {Lapčík, Petr and Stacey, Greg and Potěšil, David and Foster, Leonard and Bouchal, Pavel},
booktitle = {In Book of Abstracts of 16th Central- and Eastern European Proteomic Conference, Praha 29.9.-1.10.2022, ThO-02},
language = {eng},
title = {Global SEC-PCP-SILAC mapping reveals protein complexes mediating NF-κB activation in breast cancer},
url = {https://www.czechms.org/cz/events/cmsc/abstract.php?code=6210745},
year = {2022}
}
TY - CONF
ID - 2234357
AU - Lapčík, Petr - Stacey, Greg - Potěšil, David - Foster, Leonard - Bouchal, Pavel
PY - 2022
TI - Global SEC-PCP-SILAC mapping reveals protein complexes mediating NF-κB activation in breast cancer
UR - https://www.czechms.org/cz/events/cmsc/abstract.php?code=6210745
N2 - NF-κB has essential role in immune response and is associated with lymph node metastasis of luminal A breast tumors [1]. Analysis of protein interactome and its changes in response to NF-κB modulation could uncover pro-metastatic mechanisms related to NF-κB. We apply metabolic isotope labeling SILAC, size exclusion chromatography (SEC) and protein correlation profiling (PCP) [2] to construct a network of interactome rearrangement in response to NF-κB modulation in MCF-7 breast cancer cells. We generated two co-fractionation datasets consisting of 80 fractions from SILAC-labeled and 80 fractions from label-free native MCF-7 lysates with inhibited or native NF-κB activity. LC-MS/MS analysis of SEC fractions using Orbitrap Lumos and Bruker Impact II mass spectrometers quantified 3308 and 5460 protein groups in SILAC and label-free datasets, respectively (FDR = 0.01). Interactome reconstruction using PrinCE [3] detected 7568 interactions among 1520 proteins. Co-elution of subunits of known complexes, such as ribosome, proteasome and MCM, was observed. Modulation of NF-κB was linked to interactome changes of proteins involved in immune response, cell cycle and DNA replication. NF-κB factor RELA interacted with proteins co-eluting with activators of NF-κB and these interactions were modulated by NF-κB inhibition. Our interaction network represents a complex insight into dynamics of MCF-7 protein interactome associated with NF-κB pathway and could serv e as a basis for future studies characterizing NF-κB in breast cancer.This work was supported by Ministry of Health of the Czech Republic (grant No. NU22-08-00230) and by the project National Institute for Cancer Research (Programme EXCELES, ID Project No. LX22NPO5102) - Funded by the European Union - Next Generation EU.
ER -
LAPČÍK, Petr, Greg STACEY, David POTĚŠIL, Leonard FOSTER a Pavel BOUCHAL. Global SEC-PCP-SILAC mapping reveals protein complexes mediating NF-κB activation in breast cancer. In \textit{In Book of Abstracts of 16th Central- and Eastern European Proteomic Conference, Praha 29.9.-1.10.2022, ThO-02}. 2022.
|
