Influence of Label and Solid Support on the Performance of
Heterogeneous Immunoassays

J 2022

Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays

MAKHNEVA, Ekaterina, Dorota SKLENÁROVÁ, Julian BRANDMEIER, Antonín HLAVÁČEK, Hans-Heiner GORRIS et. al.

Základní údaje

Originální název

Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays

Autoři

MAKHNEVA, Ekaterina (643 Rusko, domácí), Dorota SKLENÁROVÁ (703 Slovensko, domácí), Julian BRANDMEIER (276 Německo, domácí), Antonín HLAVÁČEK (203 Česká republika), Hans-Heiner GORRIS (276 Německo, domácí), Petr SKLÁDAL (203 Česká republika, domácí) a Zdeněk FARKA (203 Česká republika, garant, domácí)

Vydání

Analytical Chemistry, Washington, DC, American Chemical Society, 2022, 0003-2700

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.400

Kód RIV

RIV/00216224:14310/22:00127321

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1021/acs.analchem.2c03543

UT WoS

000889895000001

Klíčová slova anglicky

Immunoassay; Magnetic preconcentration; Photon-upconversion nanoparticle; Prostate-specific antigen

Štítky

CF NANO, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 10. 2024 11:36, Ing. Monika Szurmanová, Ph.D.

Anotace

V originále

Conventional immunochemical methods used in clinical analysis are often not sensitive enough for early-stage diagnosis, resulting in the need for novel assay formats. Here, we provide a detailed comparison of the effect of different labels and solid supports on the performance of heterogeneous immunoassays. When comparing three types of streptavidin-modified labels─horseradish peroxidase, carboxyfluorescein, and photon-upconversion nanoparticles (UCNPs)─UCNPs led to the most sensitive and robust detection of the cancer biomarker prostate-specific antigen. Additionally, we compared the immunoassay formats based on conventional microtiter plates and magnetic microbeads (MBs). In both cases, the highest signal-to-background ratios and the lowest limits of detection (LODs) were obtained by using the UCNP labels. The MB-based upconversion-linked immunosorbent assay carried out with a preconcentration step provided the lowest LOD of 0.46 pg/mL in serum. The results demonstrate that the use of UCNPs and MBs can significantly improve the sensitivity and working range of heterogeneous immunoassays for biomarker detection.

Návaznosti

EF18_053/0016952, projekt VaV

Název: Postdoc2MUNI

GA21-03156S, projekt VaV

Název: Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů

Investor: Grantová agentura ČR, Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

MAKHNEVA, Ekaterina, Dorota SKLENÁROVÁ, Julian BRANDMEIER, Antonín HLAVÁČEK, Hans-Heiner GORRIS, Petr SKLÁDAL a Zdeněk FARKA. Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays. Analytical Chemistry. Washington, DC: American Chemical Society, 2022, roč. 94, č. 47, s. 16376-16383. ISSN 0003-2700. Dostupné z: https://dx.doi.org/10.1021/acs.analchem.2c03543.

@article{2236083,
   author = {Makhneva, Ekaterina and Sklenárová, Dorota and Brandmeier, Julian and Hlaváček, Antonín and Gorris, HansandHeiner and Skládal, Petr and Farka, Zdeněk},
   article_location = {Washington, DC},
   article_number = {47},
   doi = {http://dx.doi.org/10.1021/acs.analchem.2c03543},
   keywords = {Immunoassay; Magnetic preconcentration; Photon-upconversion nanoparticle; Prostate-specific antigen},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays},
   url = {https://pubs.acs.org/doi/10.1021/acs.analchem.2c03543},
   volume = {94},
   year = {2022}
}

TY  - JOUR
ID  - 2236083
AU  - Makhneva, Ekaterina - Sklenárová, Dorota - Brandmeier, Julian - Hlaváček, Antonín - Gorris, Hans-Heiner - Skládal, Petr - Farka, Zdeněk
PY  - 2022
TI  - Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays
JF  - Analytical Chemistry
VL  - 94
IS  - 47
SP  - 16376-16383
EP  - 16376-16383
PB  - American Chemical Society
SN  - 00032700
KW  - Immunoassay
KW  - Magnetic preconcentration
KW  - Photon-upconversion nanoparticle
KW  - Prostate-specific antigen
UR  - https://pubs.acs.org/doi/10.1021/acs.analchem.2c03543
N2  - Conventional immunochemical methods used in clinical analysis are often not sensitive enough for early-stage diagnosis, resulting in the need for novel assay formats. Here, we provide a detailed comparison of the effect of different labels and solid supports on the performance of heterogeneous immunoassays. When comparing three types of streptavidin-modified labels─horseradish peroxidase, carboxyfluorescein, and photon-upconversion nanoparticles (UCNPs)─UCNPs led to the most sensitive and robust detection of the cancer biomarker prostate-specific antigen. Additionally, we compared the immunoassay formats based on conventional microtiter plates and magnetic microbeads (MBs). In both cases, the highest signal-to-background ratios and the lowest limits of detection (LODs) were obtained by using the UCNP labels. The MB-based upconversion-linked immunosorbent assay carried out with a preconcentration step provided the lowest LOD of 0.46 pg/mL in serum. The results demonstrate that the use of UCNPs and MBs can significantly improve the sensitivity and working range of heterogeneous immunoassays for biomarker detection.
ER  -

MAKHNEVA, Ekaterina, Dorota SKLENÁROVÁ, Julian BRANDMEIER, Antonín HLAVÁČEK, Hans-Heiner GORRIS, Petr SKLÁDAL a Zdeněk FARKA. Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays. \textit{Analytical Chemistry}. Washington, DC: American Chemical Society, 2022, roč.~94, č.~47, s.~16376-16383. ISSN~0003-2700. Dostupné z: https://dx.doi.org/10.1021/acs.analchem.2c03543.

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