J 2022

GDF11 inhibits adipogenesis and improves mature adipocytes metabolic function via WNT/beta-catenin and ALK5/SMAD2/3 pathways

FROHLICH, Jan, Kristina KOVACOVICOVA, Marco RAFFAELE, Tereza VIRGLOVA, Eliska CIZKOVA et. al.

Základní údaje

Originální název

GDF11 inhibits adipogenesis and improves mature adipocytes metabolic function via WNT/beta-catenin and ALK5/SMAD2/3 pathways

Autoři

FROHLICH, Jan, Kristina KOVACOVICOVA, Marco RAFFAELE, Tereza VIRGLOVA, Eliska CIZKOVA, Jan KUČERA (203 Česká republika, domácí), Julie DOBROVOLNÁ (203 Česká republika, garant, domácí), Martin WABITSCH, Marion PEYROU, Francesca BONOMINI, Rita REZZANI, George N. CHALDAKOV, Anton B. TONCHEV, Michelino DI ROSA, Nicolas BLAVET (250 Francie, domácí), Václav HEJRET (203 Česká republika, domácí) a Manlio VINCIGUERRA (380 Itálie)

Vydání

Cell Proliferation, 2022, 0960-7722

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Impakt faktor

Impact factor: 8.500

Kód RIV

RIV/00216224:14310/22:00127398

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000835228000001

Klíčová slova anglicky

DIFFERENTIATION FACTOR 11; ADIPOSE-TISSUE; SKELETAL-MUSCLE; TGF-BETA; CROSS-TALK; CELL; PROTEIN; WNT; AGE; ADIPONECTIN

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 25. 10. 2024 12:44, Ing. Marie Švancarová

Anotace

V originále

GDF11 is a member of the TGF-beta superfamily that was recently implicated as potential "rejuvenating" factor, which can ameliorate metabolic disorders. The main objective of the presented study was to closely characterize the role of GDF11 signaling in the glucose homeostasis and in the differentiation of white adipose tissue. We performed microscopy imaging, biochemical and transcriptomic analyses of adipose tissues of 9 weeks old ob/ob mice and murine and human preadipocyte cell lines. Our in vivo experiments employing GDF11 treatment in ob/ob mice showed improved glucose/insulin homeostasis, decreased weight gain and white adipocyte size. Furthermore, GDF11 treatment inhibited adipogenesis in pre-adipocytes by ALK5-SMAD2/3 activation in cooperation with the WNT/beta-catenin pathway, whose inhibition resulted in adipogenic differentiation. Lastly, we observed significantly elevated levels of the adipokine hormone adiponectin and increased glucose uptake by mature adipocytes upon GDF11 exposure. We show evidence that link GDF11 to adipogenic differentiation, glucose, and insulin homeostasis, which are pointing towards potential beneficial effects of GDF11-based "anti-obesity" therapy.

Návaznosti

90267, velká výzkumná infrastruktura
Název: NCMG III