Conserved MicroRNAs in Human Nasopharynx Tissue Samples from
Swabs Are Differentially Expressed in Response to SARS-CoV-2

J 2022

Conserved MicroRNAs in Human Nasopharynx Tissue Samples from Swabs Are Differentially Expressed in Response to SARS-CoV-2

EICHMEIER, Ales, Tomas KISS, Maria KOCANOVA, Eliska HAKALOVA, Milan SPETIK et. al.

Základní údaje

Originální název

Conserved MicroRNAs in Human Nasopharynx Tissue Samples from Swabs Are Differentially Expressed in Response to SARS-CoV-2

Autoři

EICHMEIER, Ales, Tomas KISS, Maria KOCANOVA, Eliska HAKALOVA, Milan SPETIK, Jana CECHOVA a Boris TICHÝ (203 Česká republika, garant, domácí)

Vydání

GENES, MDPI, 2022, 2073-4425

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10603 Genetics and heredity

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.500

Kód RIV

RIV/00216224:14740/22:00127423

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.3390/genes13020348

UT WoS

000763155100001

Klíčová slova anglicky

miRNAs; small RNA sequencing; SARS-CoV-2; real-time RT-PCR; mir-21

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 3. 4. 2023 10:09, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

The use of high-throughput small RNA sequencing is well established as a technique to unveil the miRNAs in various tissues. The miRNA profiles are different between infected and non-infected tissues. We compare the SARS-CoV-2 positive and SARS-CoV-2 negative RNA samples extracted from human nasopharynx tissue samples to show different miRNA profiles. We explored differentially expressed miRNAs in response to SARS-CoV-2 in the RNA extracted from nasopharynx tissues of 10 SARS-CoV-2-positive and 10 SARS-CoV-2-negative patients. miRNAs were identified by small RNA sequencing, and the expression levels of selected miRNAs were validated by real-time RT-PCR. We identified 943 conserved miRNAs, likely generated through posttranscriptional modifications. The identified miRNAs were expressed in both RNA groups, NegS and PosS: miR-148a, miR-21, miR-34c, miR-34b, and miR-342. The most differentially expressed miRNA was miR-21, which is likely closely linked to the presence of SARS-CoV-2 in nasopharynx tissues. Our results contribute to further understanding the role of miRNAs in SARS-CoV-2 pathogenesis, which may be crucial for understanding disease symptom development in humans.

Citovat

EICHMEIER, Ales, Tomas KISS, Maria KOCANOVA, Eliska HAKALOVA, Milan SPETIK, Jana CECHOVA a Boris TICHÝ. Conserved MicroRNAs in Human Nasopharynx Tissue Samples from Swabs Are Differentially Expressed in Response to SARS-CoV-2. GENES. MDPI, 2022, roč. 13, č. 2, s. 348-363. ISSN 2073-4425. Dostupné z: https://dx.doi.org/10.3390/genes13020348.

@article{2239337,
   author = {Eichmeier, Ales and Kiss, Tomas and Kocanova, Maria and Hakalova, Eliska and Spetik, Milan and Cechova, Jana and Tichý, Boris},
   article_number = {2},
   doi = {http://dx.doi.org/10.3390/genes13020348},
   keywords = {miRNAs; small RNA sequencing; SARS-CoV-2; real-time RT-PCR; mir-21},
   language = {eng},
   issn = {2073-4425},
   journal = {GENES},
   title = {Conserved MicroRNAs in Human Nasopharynx Tissue Samples from Swabs Are Differentially Expressed in Response to SARS-CoV-2},
   url = {https://www.mdpi.com/2073-4425/13/2/348},
   volume = {13},
   year = {2022}
}

TY  - JOUR
ID  - 2239337
AU  - Eichmeier, Ales - Kiss, Tomas - Kocanova, Maria - Hakalova, Eliska - Spetik, Milan - Cechova, Jana - Tichý, Boris
PY  - 2022
TI  - Conserved MicroRNAs in Human Nasopharynx Tissue Samples from Swabs Are Differentially Expressed in Response to SARS-CoV-2
JF  - GENES
VL  - 13
IS  - 2
SP  - 348
EP  - 348
PB  - MDPI
SN  - 20734425
KW  - miRNAs
KW  - small RNA sequencing
KW  - SARS-CoV-2
KW  - real-time RT-PCR
KW  - mir-21
UR  - https://www.mdpi.com/2073-4425/13/2/348
N2  - The use of high-throughput small RNA sequencing is well established as a technique to unveil the miRNAs in various tissues. The miRNA profiles are different between infected and non-infected tissues. We compare the SARS-CoV-2 positive and SARS-CoV-2 negative RNA samples extracted from human nasopharynx tissue samples to show different miRNA profiles. We explored differentially expressed miRNAs in response to SARS-CoV-2 in the RNA extracted from nasopharynx tissues of 10 SARS-CoV-2-positive and 10 SARS-CoV-2-negative patients. miRNAs were identified by small RNA sequencing, and the expression levels of selected miRNAs were validated by real-time RT-PCR. We identified 943 conserved miRNAs, likely generated through posttranscriptional modifications. The identified miRNAs were expressed in both RNA groups, NegS and PosS: miR-148a, miR-21, miR-34c, miR-34b, and miR-342. The most differentially expressed miRNA was miR-21, which is likely closely linked to the presence of SARS-CoV-2 in nasopharynx tissues. Our results contribute to further understanding the role of miRNAs in SARS-CoV-2 pathogenesis, which may be crucial for understanding disease symptom development in humans.
ER  -

EICHMEIER, Ales, Tomas KISS, Maria KOCANOVA, Eliska HAKALOVA, Milan SPETIK, Jana CECHOVA a Boris TICHÝ. Conserved MicroRNAs in Human Nasopharynx Tissue Samples from Swabs Are Differentially Expressed in Response to SARS-CoV-2. \textit{GENES}. MDPI, 2022, roč.~13, č.~2, s.~348-363. ISSN~2073-4425. Dostupné z: https://dx.doi.org/10.3390/genes13020348.

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