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@article{2239337,
author = {Eichmeier, Ales and Kiss, Tomas and Kocanova, Maria and Hakalova, Eliska and Spetik, Milan and Cechova, Jana and Tichý, Boris},
article_number = {2},
doi = {http://dx.doi.org/10.3390/genes13020348},
keywords = {miRNAs; small RNA sequencing; SARS-CoV-2; real-time RT-PCR; mir-21},
language = {eng},
issn = {2073-4425},
journal = {GENES},
title = {Conserved MicroRNAs in Human Nasopharynx Tissue Samples from Swabs Are Differentially Expressed in Response to SARS-CoV-2},
url = {https://www.mdpi.com/2073-4425/13/2/348},
volume = {13},
year = {2022}
}
TY - JOUR
ID - 2239337
AU - Eichmeier, Ales - Kiss, Tomas - Kocanova, Maria - Hakalova, Eliska - Spetik, Milan - Cechova, Jana - Tichý, Boris
PY - 2022
TI - Conserved MicroRNAs in Human Nasopharynx Tissue Samples from Swabs Are Differentially Expressed in Response to SARS-CoV-2
JF - GENES
VL - 13
IS - 2
SP - 348
EP - 348
PB - MDPI
SN - 20734425
KW - miRNAs
KW - small RNA sequencing
KW - SARS-CoV-2
KW - real-time RT-PCR
KW - mir-21
UR - https://www.mdpi.com/2073-4425/13/2/348
N2 - The use of high-throughput small RNA sequencing is well established as a technique to unveil the miRNAs in various tissues. The miRNA profiles are different between infected and non-infected tissues. We compare the SARS-CoV-2 positive and SARS-CoV-2 negative RNA samples extracted from human nasopharynx tissue samples to show different miRNA profiles. We explored differentially expressed miRNAs in response to SARS-CoV-2 in the RNA extracted from nasopharynx tissues of 10 SARS-CoV-2-positive and 10 SARS-CoV-2-negative patients. miRNAs were identified by small RNA sequencing, and the expression levels of selected miRNAs were validated by real-time RT-PCR. We identified 943 conserved miRNAs, likely generated through posttranscriptional modifications. The identified miRNAs were expressed in both RNA groups, NegS and PosS: miR-148a, miR-21, miR-34c, miR-34b, and miR-342. The most differentially expressed miRNA was miR-21, which is likely closely linked to the presence of SARS-CoV-2 in nasopharynx tissues. Our results contribute to further understanding the role of miRNAs in SARS-CoV-2 pathogenesis, which may be crucial for understanding disease symptom development in humans.
ER -
EICHMEIER, Ales, Tomas KISS, Maria KOCANOVA, Eliska HAKALOVA, Milan SPETIK, Jana CECHOVA a Boris TICHÝ. Conserved MicroRNAs in Human Nasopharynx Tissue Samples from Swabs Are Differentially Expressed in Response to SARS-CoV-2. \textit{GENES}. MDPI, 2022, roč.~13, č.~2, s.~348-363. ISSN~2073-4425. Dostupné z: https://dx.doi.org/10.3390/genes13020348.
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