2022
Direct detection of TNF-alpha by copper benzene tricarboxylate MOFs/gold nanoparticles modified electrochemical label-free immunosensor using FFT admittance voltammetry
EBRAHIMI, Mehrnaz, Parviz NOROUZI, Fatemeh DAVAMI, Alireza BONAKDAR, Mahya ASGHARIAN MARZABAD et. al.Základní údaje
Originální název
Direct detection of TNF-alpha by copper benzene tricarboxylate MOFs/gold nanoparticles modified electrochemical label-free immunosensor using FFT admittance voltammetry
Autoři
EBRAHIMI, Mehrnaz, Parviz NOROUZI, Fatemeh DAVAMI, Alireza BONAKDAR, Mahya ASGHARIAN MARZABAD (364 Írán, garant, domácí) a Omid TABAEI
Vydání
Journal of Electroanalytical Chemistry, Lausanne, Elsevier, 2022, 1572-6657
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10405 Electrochemistry
Stát vydavatele
Švýcarsko
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 4.500
Kód RIV
RIV/00216224:14740/22:00127510
Organizační jednotka
Středoevropský technologický institut
UT WoS
000877512000006
Klíčová slova anglicky
TNF-? Immunoassay; Metal organic framework; Label-free electrochemical immunosensor; Gold nanoparticles; Etanercept
Štítky
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 19. 12. 2022 10:51, Mgr. Pavla Foltynová, Ph.D.
Anotace
V originále
A new simple label-free electrochemical immunosensor was designed for sensitive and selective determination of tumor necrosis factor-alpha (TNF-α), through gold nanoparticles (GNPs) incorporated copper benzene tricarboxylate metal organic frameworks (HKUST-1) modified gold electrode, where TNF-α specific monoclonal antibody was bonded to the electrode surface through shaped self-assembled monolayer (SAM) of mercapto compounds. The fabrication steps were monitored by field emission scanning electron microscopy (FE-SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under optimized experimental conditions, fast Fourier transform admittance voltammetry (FFTADV) used for TNF-α measurement. A wide linear range of current response towards the TNF-α concentration from 0.0005 to 50 ng/mL and LOD value of 0.15 pg/mL were achieved using FFTADV. The described immunosensor had adequate sensitivity and selectivity towards direct determination of TNF-α in complex biological matrix.