J 2022

Direct detection of TNF-alpha by copper benzene tricarboxylate MOFs/gold nanoparticles modified electrochemical label-free immunosensor using FFT admittance voltammetry

EBRAHIMI, Mehrnaz, Parviz NOROUZI, Fatemeh DAVAMI, Alireza BONAKDAR, Mahya ASGHARIAN MARZABAD et. al.

Základní údaje

Originální název

Direct detection of TNF-alpha by copper benzene tricarboxylate MOFs/gold nanoparticles modified electrochemical label-free immunosensor using FFT admittance voltammetry

Autoři

EBRAHIMI, Mehrnaz, Parviz NOROUZI, Fatemeh DAVAMI, Alireza BONAKDAR, Mahya ASGHARIAN MARZABAD (364 Írán, garant, domácí) a Omid TABAEI

Vydání

Journal of Electroanalytical Chemistry, Lausanne, Elsevier, 2022, 1572-6657

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Impakt faktor

Impact factor: 4.500

Kód RIV

RIV/00216224:14740/22:00127510

Organizační jednotka

Středoevropský technologický institut

UT WoS

000877512000006

Klíčová slova anglicky

TNF-? Immunoassay; Metal organic framework; Label-free electrochemical immunosensor; Gold nanoparticles; Etanercept

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 19. 12. 2022 10:51, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

A new simple label-free electrochemical immunosensor was designed for sensitive and selective determination of tumor necrosis factor-alpha (TNF-α), through gold nanoparticles (GNPs) incorporated copper benzene tricarboxylate metal organic frameworks (HKUST-1) modified gold electrode, where TNF-α specific monoclonal antibody was bonded to the electrode surface through shaped self-assembled monolayer (SAM) of mercapto compounds. The fabrication steps were monitored by field emission scanning electron microscopy (FE-SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under optimized experimental conditions, fast Fourier transform admittance voltammetry (FFTADV) used for TNF-α measurement. A wide linear range of current response towards the TNF-α concentration from 0.0005 to 50 ng/mL and LOD value of 0.15 pg/mL were achieved using FFTADV. The described immunosensor had adequate sensitivity and selectivity towards direct determination of TNF-α in complex biological matrix.