Good Practices for Histological Analysis of the Annual
Killifish Nothobranchius furzeri (Nothobranchiidae)

J 2022

Good Practices for Histological Analysis of the Annual Killifish Nothobranchius furzeri (Nothobranchiidae)

ŽÁK, Jakub, Iva DYKOVÁ, Radim BLAŽEK a Martin REICHARD

Základní údaje

Originální název

Good Practices for Histological Analysis of the Annual Killifish Nothobranchius furzeri (Nothobranchiidae)

Autoři

ŽÁK, Jakub (203 Česká republika, garant, domácí), Iva DYKOVÁ (203 Česká republika, domácí), Radim BLAŽEK (203 Česká republika, domácí) a Martin REICHARD (203 Česká republika, domácí)

Vydání

Cold Spring Harbor Protocols, Cold Spring Harbor Laboratory Press, 2022, 1940-3402

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14310/22:00127773

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1101/pdb.prot107739

Klíčová slova anglicky

Animals; Eosine Yellowish-(YS); Fundulidae; Microtomy; Paraffin; Staining and Labeling

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 3. 4. 2023 11:43, Mgr. Marie Šípková, DiS.

Anotace

V originále

Paraffin histology is one of the most important and commonly used laboratory techniques enabling the study of the microscopic structure of animal and plant tissues. This technique uses paraffin wax, which in liquid form impregnates fixed and dehydrated tissues and allows the preparation of thin sections when solidified in blocks. This protocol on good practices in paraffin histology of Nothobranchius furzeri (Nothobranchiidae) summarizes the authors' current experience in terms of technique, evaluation, and interpretation of sectioned tissues. The steps that precede paraffin block preparation are also presented as they play a key role in maximizing the quality of examined sections. The paraffin technique as described only requires basic laboratory conditions to produce good-quality results. The description of staining methods is limited to Mayer's hematoxylin and eosin (H&E), the routinely used histological dye staining cell nuclei in blue-black (hematein) and cell cytoplasm and connective tissue fibers in shades of pink-red (eosin). Killifish specialists are encouraged to engage in the study of histology and histopathology, taking advantage of interdisciplinary cooperation. © 2022 Cold Spring Harbor Laboratory Press.

Citovat

ŽÁK, Jakub, Iva DYKOVÁ, Radim BLAŽEK a Martin REICHARD. Good Practices for Histological Analysis of the Annual Killifish Nothobranchius furzeri (Nothobranchiidae). Cold Spring Harbor Protocols. Cold Spring Harbor Laboratory Press, 2022, roč. 2022, č. 11, s. 574-583. ISSN 1940-3402. Dostupné z: https://dx.doi.org/10.1101/pdb.prot107739.

@article{2244098,
   author = {Žák, Jakub and Dyková, Iva and Blažek, Radim and Reichard, Martin},
   article_number = {11},
   doi = {http://dx.doi.org/10.1101/pdb.prot107739},
   keywords = {Animals; Eosine Yellowish-(YS); Fundulidae; Microtomy; Paraffin; Staining and Labeling},
   language = {eng},
   issn = {1940-3402},
   journal = {Cold Spring Harbor Protocols},
   title = {Good Practices for Histological Analysis of the Annual Killifish Nothobranchius furzeri (Nothobranchiidae)},
   url = {http://cshprotocols.cshlp.org/content/early/2022/08/02/pdb.prot107739},
   volume = {2022},
   year = {2022}
}

TY  - JOUR
ID  - 2244098
AU  - Žák, Jakub - Dyková, Iva - Blažek, Radim - Reichard, Martin
PY  - 2022
TI  - Good Practices for Histological Analysis of the Annual Killifish Nothobranchius furzeri (Nothobranchiidae)
JF  - Cold Spring Harbor Protocols
VL  - 2022
IS  - 11
SP  - 574-583
EP  - 574-583
PB  - Cold Spring Harbor Laboratory Press
SN  - 19403402
KW  - Animals
KW  - Eosine Yellowish-(YS)
KW  - Fundulidae
KW  - Microtomy
KW  - Paraffin
KW  - Staining and Labeling
UR  - http://cshprotocols.cshlp.org/content/early/2022/08/02/pdb.prot107739
N2  - Paraffin histology is one of the most important and commonly used laboratory techniques enabling the study of the microscopic structure of animal and plant tissues. This technique uses paraffin wax, which in liquid form impregnates fixed and dehydrated tissues and allows the preparation of thin sections when solidified in blocks. This protocol on good practices in paraffin histology of Nothobranchius furzeri (Nothobranchiidae) summarizes the authors' current experience in terms of technique, evaluation, and interpretation of sectioned tissues. The steps that precede paraffin block preparation are also presented as they play a key role in maximizing the quality of examined sections. The paraffin technique as described only requires basic laboratory conditions to produce good-quality results. The description of staining methods is limited to Mayer's hematoxylin and eosin (H&E), the routinely used histological dye staining cell nuclei in blue-black (hematein) and cell cytoplasm and connective tissue fibers in shades of pink-red (eosin). Killifish specialists are encouraged to engage in the study of histology and histopathology, taking advantage of interdisciplinary cooperation. © 2022 Cold Spring Harbor Laboratory Press.
ER  -

ŽÁK, Jakub, Iva DYKOVÁ, Radim BLAŽEK a Martin REICHARD. Good Practices for Histological Analysis of the Annual Killifish Nothobranchius furzeri (Nothobranchiidae). \textit{Cold Spring Harbor Protocols}. Cold Spring Harbor Laboratory Press, 2022, roč.~2022, č.~11, s.~574-583. ISSN~1940-3402. Dostupné z: https://dx.doi.org/10.1101/pdb.prot107739.

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