Good Practices for Histological Analysis of the Annual Killifish Nothobranchius furzeri (Nothobranchiidae)

ŽÁK, Jakub, Iva DYKOVÁ, Radim BLAŽEK and Martin REICHARD. Good Practices for Histological Analysis of the Annual Killifish Nothobranchius furzeri (Nothobranchiidae). Cold Spring Harbor Protocols. Cold Spring Harbor Laboratory Press, 2022, vol. 2022, No 11, p. 574-583. ISSN 1940-3402. Available from: https://dx.doi.org/10.1101/pdb.prot107739.

Basic information

• Original name: Good Practices for Histological Analysis of the Annual Killifish Nothobranchius furzeri (Nothobranchiidae)
• Authors: ŽÁK, Jakub (203 Czech Republic, guarantor, belonging to the institution), Iva DYKOVÁ (203 Czech Republic, belonging to the institution), Radim BLAŽEK (203 Czech Republic, belonging to the institution) and Martin REICHARD (203 Czech Republic, belonging to the institution).
• Edition: Cold Spring Harbor Protocols, Cold Spring Harbor Laboratory Press, 2022, 1940-3402.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14310/22:00127773
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1101/pdb.prot107739
• Keywords in English: Animals; Eosine Yellowish-(YS); Fundulidae; Microtomy; Paraffin; Staining and Labeling
• Tags: rivok
• Tags: International impact, Reviewed

Abstract

Paraffin histology is one of the most important and commonly used laboratory techniques enabling the study of the microscopic structure of animal and plant tissues. This technique uses paraffin wax, which in liquid form impregnates fixed and dehydrated tissues and allows the preparation of thin sections when solidified in blocks. This protocol on good practices in paraffin histology of Nothobranchius furzeri (Nothobranchiidae) summarizes the authors' current experience in terms of technique, evaluation, and interpretation of sectioned tissues. The steps that precede paraffin block preparation are also presented as they play a key role in maximizing the quality of examined sections. The paraffin technique as described only requires basic laboratory conditions to produce good-quality results. The description of staining methods is limited to Mayer's hematoxylin and eosin (H&E), the routinely used histological dye staining cell nuclei in blue-black (hematein) and cell cytoplasm and connective tissue fibers in shades of pink-red (eosin). Killifish specialists are encouraged to engage in the study of histology and histopathology, taking advantage of interdisciplinary cooperation. © 2022 Cold Spring Harbor Laboratory Press.