Testing of antimicrobial effect of hydrogel matrix based on Gum
Karaya resin supplemented by the phage preparation on
methicillin-resistant  Staphylococcus aureus strains on animal
model.

k 2022

Testing of antimicrobial effect of hydrogel matrix based on Gum Karaya resin supplemented by the phage preparation on methicillin-resistant Staphylococcus aureus strains on animal model.

KLEKNEROVÁ, Dominika, Lukáš VACEK, Alena SIVÁKOVÁ, Roman PANTŮČEK, Martin BENEŠÍK et. al.

Základní údaje

Originální název

Testing of antimicrobial effect of hydrogel matrix based on Gum Karaya resin supplemented by the phage preparation on methicillin-resistant Staphylococcus aureus strains on animal model.

Autoři

KLEKNEROVÁ, Dominika (703 Slovensko, domácí), Lukáš VACEK (203 Česká republika, domácí), Alena SIVÁKOVÁ (203 Česká republika, domácí), Roman PANTŮČEK (203 Česká republika, domácí), Martin BENEŠÍK (203 Česká republika, domácí), Lucy VOJTOVÁ (203 Česká republika) a Eva ČERNÁ (203 Česká republika)

Vydání

Hot Topics in Microbiology - International Conference for Young Scientists, 2022

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

10606 Microbiology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14110/22:00129725

Organizační jednotka

Lékařská fakulta

ISBN

978-80-973411-4-5

Klíčová slova česky

MRSA; fágy; Gum Karaya; hojenie rán; nové antimikrobiálne prístupy

Klíčová slova anglicky

MRSA; phages; Gum Karaya; wound healing; new antimicrobial approaches

Štítky

rivok

Příznaky

Mezinárodní význam

Změněno: 9. 5. 2023 09:12, Mgr. Tereza Miškechová

Anotace

V originále

Introduction Gum karaya (GK) is a natural polysaccharide with great potential in the treatment of chronic skin and soft tissue infections (SSTIs). The polysaccharide-based hydrogels keep a moist environment and stimulate faster moist wound healing. Supplementing this material with phage preparation can increase its antimicrobial effects. Methodology Four pigs (20 wounds, 5x5cm) were used in the experiment. The wounds were infected with Staphylococcus aureus (ST22). The first sampling verified a successful infection. The other 4 samplings monitored the treatment. A piece of tissue was taken as well as an impression of the wound surface. The tissue was processed and 10 µl of the suspension was added to the BA and MH with oxacillin. The impression was transferred from BA to MH. The plates were cultured at 37°C for 48 h. Then the colonies were counted, and numbers were converted to tissue weight (CFU/g). The impressions were scored quantitatively using a 1-4 scale. Results Testing showed a positive effect of the combination of GK with phage in both tissue and surface wounds. During the experiment, there was a gradual reduction of bacterial numbers in the tissues and in the surface impressions. The wound was gradually shrinking by the formation of healthy tissue. Conclusion GK-based hydrogels have been tested in vitro, where they showed an antimicrobial effect. These experiments in an animal model show that the effect is also demonstrated directly on the infected wound. This material could be a promising candidate for the treatment of chronic wounds. This abstract has been supported by the grants NU20-05-00166 and NU22-05-00475 (The Ministry of Health of the Czech Republic).

Návaznosti

NU20-05-00166, projekt VaV

Název: Nové přístupy k hojení infikovaných ran pomocí hydrogelů z přírodní pryskyřice Gum Karaya modifikovaných inovativními komponenty cílenými na multirezistentní kmeny bakterií (Akronym: NPHIRPHGK)

Investor: Ministerstvo zdravotnictví ČR, Nové přístupy k hojení infikovaných ran pomocí hydrogelů z přírodní pryskyřice Gum Karaya modifikovaných inovativními komponenty cílenými na multirezistentní kmeny bakterií, Podprogram 1 - standardní

Citovat

KLEKNEROVÁ, Dominika, Lukáš VACEK, Alena SIVÁKOVÁ, Roman PANTŮČEK, Martin BENEŠÍK, Lucy VOJTOVÁ a Eva ČERNÁ. Testing of antimicrobial effect of hydrogel matrix based on Gum Karaya resin supplemented by the phage preparation on methicillin-resistant Staphylococcus aureus strains on animal model. In Hot Topics in Microbiology - International Conference for Young Scientists. 2022. ISBN 978-80-973411-4-5.

@proceedings{2245083,
   author = {Kleknerová, Dominika and Vacek, Lukáš and Siváková, Alena and Pantůček, Roman and Benešík, Martin and Vojtová, Lucy and Černá, Eva},
   booktitle = {Hot Topics in Microbiology - International Conference for Young Scientists},
   keywords = {MRSA; phages; Gum Karaya; wound healing; new antimicrobial approaches},
   language = {eng},
   isbn = {978-80-973411-4-5},
   title = {Testing of antimicrobial effect of hydrogel matrix based on Gum Karaya resin supplemented by the phage preparation on methicillin-resistant Staphylococcus aureus strains on animal model.},
   url = {https://cembo.eu/cembo-conference-2022/},
   year = {2022}
}

TY  - CONF
ID  - 2245083
AU  - Kleknerová, Dominika - Vacek, Lukáš - Siváková, Alena - Pantůček, Roman - Benešík, Martin - Vojtová, Lucy - Černá, Eva
PY  - 2022
TI  - Testing of antimicrobial effect of hydrogel matrix based on Gum Karaya resin supplemented by the phage preparation on methicillin-resistant Staphylococcus aureus strains on animal model.
SN  - 9788097341145
KW  - MRSA
KW  - phages
KW  - Gum Karaya
KW  - wound healing
KW  - new antimicrobial approaches
UR  - https://cembo.eu/cembo-conference-2022/
N2  - Introduction Gum karaya (GK) is a natural polysaccharide with great potential in the treatment of chronic skin and soft tissue infections (SSTIs). The polysaccharide-based hydrogels keep a moist environment and stimulate faster moist wound healing. Supplementing this material with phage preparation can increase its antimicrobial effects. Methodology Four pigs (20 wounds, 5x5cm) were used in the experiment. The wounds were infected with Staphylococcus aureus (ST22). The first sampling verified a successful infection. The other 4 samplings monitored the treatment. A piece of tissue was taken as well as an impression of the wound surface. The tissue was processed and 10 µl of the suspension was added to the BA and MH with oxacillin. The impression was transferred from BA to MH. The plates were cultured at 37°C for 48 h. Then the colonies were counted, and numbers were converted to tissue weight (CFU/g). The impressions were scored quantitatively using a 1-4 scale. Results Testing showed a positive effect of the combination of GK with phage in both tissue and surface wounds. During the experiment, there was a gradual reduction of bacterial numbers in the tissues and in the surface impressions. The wound was gradually shrinking by the formation of healthy tissue. Conclusion GK-based hydrogels have been tested in vitro, where they showed an antimicrobial effect. These experiments in an animal model show that the effect is also demonstrated directly on the infected wound. This material could be a promising candidate for the treatment of chronic wounds. This abstract has been supported by the grants NU20-05-00166 and NU22-05-00475 (The Ministry of Health of the Czech Republic).
ER  -

KLEKNEROVÁ, Dominika, Lukáš VACEK, Alena SIVÁKOVÁ, Roman PANTŮČEK, Martin BENEŠÍK, Lucy VOJTOVÁ a Eva ČERNÁ. Testing of antimicrobial effect of hydrogel matrix based on Gum Karaya resin supplemented by the phage preparation on methicillin-resistant Staphylococcus aureus strains on animal model. In \textit{Hot Topics in Microbiology - International Conference for Young Scientists}. 2022. ISBN~978-80-973411-4-5.

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