2022
Changes in CXCR4 and CXCR7 in the anterior cingulate cortex neurons of the neuropathic pain model
JAMBRICHOVÁ, Anna, Karolína BRETOVÁ, Anna BAGÓ MAS, Pere BOADAS-VAELLO, Petr DUBOVÝ et. al.Základní údaje
Originální název
Changes in CXCR4 and CXCR7 in the anterior cingulate cortex neurons of the neuropathic pain model
Název anglicky
Changes in CXCR4 and CXCR7 in the anterior cingulate cortex neurons of the neuropathic pain model
Autoři
JAMBRICHOVÁ, Anna, Karolína BRETOVÁ, Anna BAGÓ MAS, Pere BOADAS-VAELLO a Petr DUBOVÝ
Vydání
2022
Další údaje
Typ výsledku
Konferenční abstrakt
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Organizační jednotka
Lékařská fakulta
Klíčová slova anglicky
chemokine receptors; cortex; spinal cord contusion
Změněno: 20. 2. 2023 09:23, Mgr. Tereza Miškechová
V originále
The anterior cingulate cortex (ACC) is a critical brain region that plays a role in affective components of neuropathic pain. Functional signaling of chemokine CXCL12 is mediated by CXCR4 and modulated by a scavenger receptor CXCR7. Our experiments aimed to investigate changes in CXCR4 and CXCR7 protein levels in the ACC neurons of the mouse model of neuropathic pain based on spinal cord injury (SCI). We analyzed the effects of polyphenolic compounds contained in grape stalk extract (GSE) and coffee extract (CE). Immunofluorescence staining was used to detect the cellular distribution of CXCR4 and CXCR7 and their quantification in the ACC neurons of lamina II and III from naïve, sham and SCI-operated mice as well as those treated with GSE and CE. CXCR7 and CXCR4 were detected only in the ACC neurons of both laminae. Sham-operation induced significant increase of CXCR4 levels compared to those of the naïve mice. Surprisingly, SCI compared to sham-operation resulted in decreased levels of CXCR4 protein. Although GSE treatment did not change CXCR4 level, CE led to a significant increase of this receptor in the ACC neurons. CXCR4 immunofluorescence intensities differed from those of CXCR7. Sham-operation resulted in significantly reduced CXCR7 levels that were decreased also in the SCI-saline group of mice without difference in the laminae. Treatment with GSE led to resemblance of CXCR7 level to that detected in naïve animals. In contrast, CE treatment caused significant decrease of CXCR7 compared to the ACC neurons in the SCI-saline group of mice. Our results suggested differences of CXCR7 levels in the laminae, while this was not found in the CXCR4. We demonstrated various effects of GSE and CE on the levels of CXCR4 and CXCR7 in the ACC neurons of SCI-operated mice.
Anglicky
The anterior cingulate cortex (ACC) is a critical brain region that plays a role in affective components of neuropathic pain. Functional signaling of chemokine CXCL12 is mediated by CXCR4 and modulated by a scavenger receptor CXCR7. Our experiments aimed to investigate changes in CXCR4 and CXCR7 protein levels in the ACC neurons of the mouse model of neuropathic pain based on spinal cord injury (SCI). We analyzed the effects of polyphenolic compounds contained in grape stalk extract (GSE) and coffee extract (CE). Immunofluorescence staining was used to detect the cellular distribution of CXCR4 and CXCR7 and their quantification in the ACC neurons of lamina II and III from naïve, sham and SCI-operated mice as well as those treated with GSE and CE. CXCR7 and CXCR4 were detected only in the ACC neurons of both laminae. Sham-operation induced significant increase of CXCR4 levels compared to those of the naïve mice. Surprisingly, SCI compared to sham-operation resulted in decreased levels of CXCR4 protein. Although GSE treatment did not change CXCR4 level, CE led to a significant increase of this receptor in the ACC neurons. CXCR4 immunofluorescence intensities differed from those of CXCR7. Sham-operation resulted in significantly reduced CXCR7 levels that were decreased also in the SCI-saline group of mice without difference in the laminae. Treatment with GSE led to resemblance of CXCR7 level to that detected in naïve animals. In contrast, CE treatment caused significant decrease of CXCR7 compared to the ACC neurons in the SCI-saline group of mice. Our results suggested differences of CXCR7 levels in the laminae, while this was not found in the CXCR4. We demonstrated various effects of GSE and CE on the levels of CXCR4 and CXCR7 in the ACC neurons of SCI-operated mice.
Návaznosti
| MUNI/A/1331/2021, interní kód MU |
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