Transcriptome and proteome associated analysis of flavonoid
metabolism in haploid Ginkgo biloba

J 2023

Transcriptome and proteome associated analysis of flavonoid metabolism in haploid Ginkgo biloba

HU, Yaping, Yun ZHANG, Petr ŠMARDA, Petr BUREŠ, Qirong GUO et. al.

Základní údaje

Originální název

Transcriptome and proteome associated analysis of flavonoid metabolism in haploid Ginkgo biloba

Autoři

HU, Yaping (156 Čína), Yun ZHANG (156 Čína), Petr ŠMARDA (203 Česká republika, domácí), Petr BUREŠ (203 Česká republika, domácí) a Qirong GUO (156 Čína, garant)

Vydání

International Journal of Biological Macromolecules, Elsevier, 2023, 0141-8130

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 8.200 v roce 2022

Kód RIV

RIV/00216224:14310/23:00134043

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.ijbiomac.2022.10.125

UT WoS

000906782900001

Klíčová slova anglicky

Flavonoid content; Gene dose; Haploid ginkgo; Hub genes; Proteome; Transcriptome

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 6. 1. 2024 00:22, prof. RNDr. Petr Bureš, Ph.D.

Anotace

V originále

Having different number if genome copies affect transcription and metabolite production of plants. This may be due to different gene transcription and protein expression, but the reasons for this remains poorly known. Here we measured flavonoid content in leaves of three haploid and diploid grafted plants of Ginkgo biloba, a model gymnosperm important economically for its flavonoid content. We reported the first combined transcriptomic and proteomic analysis of the difference in flavonoid content in three haploid ginkgos to investigate the effect of haploidy. Haploids had always smaller leaves and flavonoid content than the diploids. The selected haploid had also generally lower gene dosage than the selected diploid, with 1149 up-regulated (46.8 %) and 1309 down-regulated (53.2 %) among 2452 differentially expressed genes (DEGs). Of 686 differentially expressed proteins (DEPs) detected, 289 proteins (42.1 %) were upregulated, and 397 proteins (57.9 %) were downregulated in haploids. A particular attention deserves the downregulation of PAL, PAM, FLS, OMT1 hub genes involved in flavonoid biosynthesis regulation. Our study confirms the trend of haploids to have lower metabolic contents and points that lower flavonoid content in ginkgo monoploids could be due to reduced dosage of the corresponding regulatory genes and downregulation of genes involved in flavonoid synthesis.

Návaznosti

GA19-18545S, projekt VaV

Název: Eko-geografická limitace rostlinných polyploidů: experimentální testování nových hypotéz souvisejících s velikostí buněk

Investor: Grantová agentura ČR, Eko-geografická limitace rostlinných polyploidů: experimentální testování nových hypotéz souvisejících s velikostí buněk

Citovat

HU, Yaping, Yun ZHANG, Petr ŠMARDA, Petr BUREŠ a Qirong GUO. Transcriptome and proteome associated analysis of flavonoid metabolism in haploid Ginkgo biloba. International Journal of Biological Macromolecules. Elsevier, 2023, roč. 224, January, s. 306-318. ISSN 0141-8130. Dostupné z: https://dx.doi.org/10.1016/j.ijbiomac.2022.10.125.

@article{2245803,
   author = {Hu, Yaping and Zhang, Yun and Šmarda, Petr and Bureš, Petr and Guo, Qirong},
   article_number = {January},
   doi = {http://dx.doi.org/10.1016/j.ijbiomac.2022.10.125},
   keywords = {Flavonoid content; Gene dose; Haploid ginkgo; Hub genes; Proteome; Transcriptome},
   language = {eng},
   issn = {0141-8130},
   journal = {International Journal of Biological Macromolecules},
   title = {Transcriptome and proteome associated analysis of flavonoid metabolism in haploid Ginkgo biloba},
   url = {https://doi.org/10.1016/j.ijbiomac.2022.10.125},
   volume = {224},
   year = {2023}
}

TY  - JOUR
ID  - 2245803
AU  - Hu, Yaping - Zhang, Yun - Šmarda, Petr - Bureš, Petr - Guo, Qirong
PY  - 2023
TI  - Transcriptome and proteome associated analysis of flavonoid metabolism in haploid Ginkgo biloba
JF  - International Journal of Biological Macromolecules
VL  - 224
IS  - January
SP  - 306-318
EP  - 306-318
PB  - Elsevier
SN  - 01418130
KW  - Flavonoid content
KW  - Gene dose
KW  - Haploid ginkgo
KW  - Hub genes
KW  - Proteome
KW  - Transcriptome
UR  - https://doi.org/10.1016/j.ijbiomac.2022.10.125
N2  - Having different number if genome copies affect transcription and metabolite production of plants. This may be due to different gene transcription and protein expression, but the reasons for this remains poorly known. Here we measured flavonoid content in leaves of three haploid and diploid grafted plants of Ginkgo biloba, a model gymnosperm important economically for its flavonoid content. We reported the first combined transcriptomic and proteomic analysis of the difference in flavonoid content in three haploid ginkgos to investigate the effect of haploidy. Haploids had always smaller leaves and flavonoid content than the diploids. The selected haploid had also generally lower gene dosage than the selected diploid, with 1149 up-regulated (46.8 %) and 1309 down-regulated (53.2 %) among 2452 differentially expressed genes (DEGs). Of 686 differentially expressed proteins (DEPs) detected, 289 proteins (42.1 %) were upregulated, and 397 proteins (57.9 %) were downregulated in haploids. A particular attention deserves the downregulation of PAL, PAM, FLS, OMT1 hub genes involved in flavonoid biosynthesis regulation. Our study confirms the trend of haploids to have lower metabolic contents and points that lower flavonoid content in ginkgo monoploids could be due to reduced dosage of the corresponding regulatory genes and downregulation of genes involved in flavonoid synthesis.
ER  -

HU, Yaping, Yun ZHANG, Petr ŠMARDA, Petr BUREŠ a Qirong GUO. Transcriptome and proteome associated analysis of flavonoid metabolism in haploid Ginkgo biloba. \textit{International Journal of Biological Macromolecules}. Elsevier, 2023, roč.~224, January, s.~306-318. ISSN~0141-8130. Dostupné z: https://dx.doi.org/10.1016/j.ijbiomac.2022.10.125.

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