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@inproceedings{224633,
author = {Kozubek, Michal and Kozubek, Stanislav and Lukášová, Emilie and Amrichová, Jana and Rýznar, Leonard and Lišková, Andrea},
address = {New York},
booktitle = {Fluorescence Microscopy and Fluorescent Probes},
edition = {vol.2},
keywords = {image analysis; automated microscopy; fluorescence in situ hybridization; interphase nuclei},
language = {eng},
location = {New York},
isbn = {0-306-46021-1},
pages = {173-179},
publisher = {Plenum},
title = {Semi-automated FISH-stained interphase nuclei analysis in human genome studies},
year = {1998}
}
TY - JOUR
ID - 224633
AU - Kozubek, Michal - Kozubek, Stanislav - Lukášová, Emilie - Amrichová, Jana - Rýznar, Leonard - Lišková, Andrea
PY - 1998
TI - Semi-automated FISH-stained interphase nuclei analysis in human genome studies
PB - Plenum
CY - New York
SN - 0306460211
KW - image analysis
KW - automated microscopy
KW - fluorescence in situ hybridization
KW - interphase nuclei
N2 - We have used fluorescence in situ hybridization (FISH) technique for the investigation of the arrangement of specific genes and chromosomes in human cells. Lymphocytes, bone marrow cells, human fibroblasts and other cell lines were studied. Results for normal and irradiated cells were compared in order to estimate the influence of radiation on the genes' arrangement. We compared also the difference in genes' arrangement between healthy donors and patients suffering from a specific disease related to a certain type of chromosomal aberration, such as BCR/ABL gene in the case of chronic myeloid leukaemia. Both epi-fluorescence and confocal microscopes were used for the observation. Both two-dimensional (2D) and three-dimensional (3D) analysis of interphase nuclei was performed. Besides, an algorithm for the estimation of 3D structure form 2D projections was proposed. The localization of interphase nuclei in the microscope was performed manually, image capturing and storage semi-automatically. Computer analysis was full-automatic with the possibility to analyse whole stack of images overnight. The results of the automatic analysis were verified visually on the computer monitor and corrected where necessary. The whole system is being rebuilt now into a full-automatic version based on Leica DMRXA motorized computer driven microscope. Algorithms for the image analysis such as nuclei and genes' segmentation as well as biological results of our studies are presented.
ER -
KOZUBEK, Michal, Stanislav KOZUBEK, Emilie LUKÁŠOVÁ, Jana AMRICHOVÁ, Leonard RÝZNAR and Andrea LIŠKOVÁ. Semi-automated FISH-stained interphase nuclei analysis in human genome studies. edited by J.Slavík. In \textit{Fluorescence Microscopy and Fluorescent Probes}. vol.2. New York: Plenum. p.~173-179. ISBN~0-306-46021-1. 1998.
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