Quantification and identification of bile acids in saliva by
liquid chromatography-mass spectrometry: Possible non-invasive
diagnostics of Barrett's esophagus?

J 2022

Quantification and identification of bile acids in saliva by liquid chromatography-mass spectrometry: Possible non-invasive diagnostics of Barrett's esophagus?

DOSEDĚLOVÁ, Věra, Marketa LASTOVICKOVA, Juan F AYALA-CABRERA, Jiří DOLINA, Štefan KONEČNÝ et. al.

Základní údaje

Originální název

Quantification and identification of bile acids in saliva by liquid chromatography-mass spectrometry: Possible non-invasive diagnostics of Barrett's esophagus?

Autoři

DOSEDĚLOVÁ, Věra (203 Česká republika, domácí), Marketa LASTOVICKOVA (203 Česká republika), Juan F AYALA-CABRERA, Jiří DOLINA (203 Česká republika, domácí), Štefan KONEČNÝ (703 Slovensko, domácí), Oliver J. SCHMITZ a Petr KUBÁŇ (203 Česká republika)

Vydání

Journal of Chromatography A, Amsterdam, ELSEVIER SCIENCE BV, 2022, 0021-9673

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30219 Gastroenterology and hepatology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.100

Kód RIV

RIV/00216224:14740/22:00128067

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.chroma.2022.463287

UT WoS

000834927200006

Klíčová slova anglicky

Barrett’s esophagus; Bileacids; LC-MS; Salivaanalysis; Solid-phaseextraction

Štítky

14110213, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 2. 2023 10:00, Mgr. Tereza Miškechová

Anotace

V originále

Bile acids are a group of steroid compounds essential for lipid digestion. However, when bile acids are refluxed into the stomach and the esophagus, during the so called duodenogastroesophageal reflux, they can have a detrimental effect on the esophageal epithelium and cause pathological changes of esophageal tissue, e.g., Barrett's esophagus (BE). The levels of bile acids in saliva could therefore serve as possi-ble biomarkers for the diagnostics of BE. In this work, we focused on optimization of sample collection and preparation by solid-phase extraction and subsequent quantification of 11 bile acids (unconjugated, glycine-conjugated) in saliva from healthy volunteers and BE patients by ultra-high-performance liquid chromatography coupled to triple-quadrupole tandem mass spectrometry. Moreover, high resolution MS (Orbitrap-MS) was utilized for identification of new bile acids in saliva. Methods for saliva collection in-cluding simple spitting and the Salivette (R) saliva collection system were compared; the latter was found to be unsuitable due to excessive retention of bile acids in the cotton swab. Methanol with 0.1% formic acid were selected for protein precipitation and bile acid extraction prior to SPE. Separation was per-formed in gradient elution of methanol and 0.1% formic acid in less than 10 min. Saliva from BE patients contained higher levels of almost all bile acids, and the tested groups could be distinguished by princi-pal component analysis. In untargeted analysis by high resolution MS, taurine-conjugated bile acids and glycine-conjugated dihydroxy-bile acid sulfate were identified in saliva from healthy volunteers. We pro-pose that analysis of salivary bile acids including taurine conjugates could be applicable in diagnostics of BE, following a larger clinical study.

Citovat

DOSEDĚLOVÁ, Věra, Marketa LASTOVICKOVA, Juan F AYALA-CABRERA, Jiří DOLINA, Štefan KONEČNÝ, Oliver J. SCHMITZ a Petr KUBÁŇ. Quantification and identification of bile acids in saliva by liquid chromatography-mass spectrometry: Possible non-invasive diagnostics of Barrett's esophagus? Journal of Chromatography A. Amsterdam: ELSEVIER SCIENCE BV, 2022, roč. 1676, August 2022, s. 1-13. ISSN 0021-9673. Dostupné z: https://dx.doi.org/10.1016/j.chroma.2022.463287.

@article{2247520,
   author = {Dosedělová, Věra and Lastovickova, Marketa and AyalaandCabrera, Juan F and Dolina, Jiří and Konečný, Štefan and Schmitz, Oliver J. and Kubáň, Petr},
   article_location = {Amsterdam},
   article_number = {August 2022},
   doi = {http://dx.doi.org/10.1016/j.chroma.2022.463287},
   keywords = {Barrett’s esophagus; Bileacids; LC-MS; Salivaanalysis; Solid-phaseextraction},
   language = {eng},
   issn = {0021-9673},
   journal = {Journal of Chromatography A},
   title = {Quantification and identification of bile acids in saliva by liquid chromatography-mass spectrometry: Possible non-invasive diagnostics of Barrett's esophagus?},
   url = {https://www.sciencedirect.com/science/article/pii/S0021967322004800?via%3Dihub#keys0001},
   volume = {1676},
   year = {2022}
}

TY  - JOUR
ID  - 2247520
AU  - Dosedělová, Věra - Lastovickova, Marketa - Ayala-Cabrera, Juan F - Dolina, Jiří - Konečný, Štefan - Schmitz, Oliver J. - Kubáň, Petr
PY  - 2022
TI  - Quantification and identification of bile acids in saliva by liquid chromatography-mass spectrometry: Possible non-invasive diagnostics of Barrett's esophagus?
JF  - Journal of Chromatography A
VL  - 1676
IS  - August 2022
SP  - 1-13
EP  - 1-13
PB  - ELSEVIER SCIENCE BV
SN  - 00219673
KW  - Barrett’s esophagus
KW  - Bileacids
KW  - LC-MS
KW  - Salivaanalysis
KW  - Solid-phaseextraction
UR  - https://www.sciencedirect.com/science/article/pii/S0021967322004800?via%3Dihub#keys0001
N2  - Bile acids are a group of steroid compounds essential for lipid digestion. However, when bile acids are refluxed into the stomach and the esophagus, during the so called duodenogastroesophageal reflux, they can have a detrimental effect on the esophageal epithelium and cause pathological changes of esophageal tissue, e.g., Barrett's esophagus (BE). The levels of bile acids in saliva could therefore serve as possi-ble biomarkers for the diagnostics of BE. In this work, we focused on optimization of sample collection and preparation by solid-phase extraction and subsequent quantification of 11 bile acids (unconjugated, glycine-conjugated) in saliva from healthy volunteers and BE patients by ultra-high-performance liquid chromatography coupled to triple-quadrupole tandem mass spectrometry. Moreover, high resolution MS (Orbitrap-MS) was utilized for identification of new bile acids in saliva. Methods for saliva collection in-cluding simple spitting and the Salivette (R) saliva collection system were compared; the latter was found to be unsuitable due to excessive retention of bile acids in the cotton swab. Methanol with 0.1% formic acid were selected for protein precipitation and bile acid extraction prior to SPE. Separation was per-formed in gradient elution of methanol and 0.1% formic acid in less than 10 min. Saliva from BE patients contained higher levels of almost all bile acids, and the tested groups could be distinguished by princi-pal component analysis. In untargeted analysis by high resolution MS, taurine-conjugated bile acids and glycine-conjugated dihydroxy-bile acid sulfate were identified in saliva from healthy volunteers. We pro-pose that analysis of salivary bile acids including taurine conjugates could be applicable in diagnostics of BE, following a larger clinical study.
ER  -

DOSEDĚLOVÁ, Věra, Marketa LASTOVICKOVA, Juan F AYALA-CABRERA, Jiří DOLINA, Štefan KONEČNÝ, Oliver J. SCHMITZ a Petr KUBÁŇ. Quantification and identification of bile acids in saliva by liquid chromatography-mass spectrometry: Possible non-invasive diagnostics of Barrett's esophagus? \textit{Journal of Chromatography A}. Amsterdam: ELSEVIER SCIENCE BV, 2022, roč.~1676, August 2022, s.~1-13. ISSN~0021-9673. Dostupné z: https://dx.doi.org/10.1016/j.chroma.2022.463287.

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