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@article{224771,
author = {Juškaitis, Rimas and Wilson, Tony and Neil, Mark and Kozubek, Michal},
article_number = {6603},
keywords = {confocal microscopy; SCAM},
language = {eng},
issn = {0028-0836},
journal = {Nature},
title = {Efficient real-time confocal microscopy with white light sources},
volume = {383},
year = {1996}
}
TY - JOUR
ID - 224771
AU - Juškaitis, Rimas - Wilson, Tony - Neil, Mark - Kozubek, Michal
PY - 1996
TI - Efficient real-time confocal microscopy with white light sources
JF - Nature
VL - 383
IS - 6603
SP - 804
EP - 804
SN - 00280836
KW - confocal microscopy
KW - SCAM
N2 - The main advantage of confocal microscopes over their conventional counterparts arises from their ability to optically 'section' nearly transparent materials; the thin image slices thus obtained can be used to reconstruct three-dimensional images, a capability which is particularly useful for the study of biological specimens. Confocal microscopes have previously used either a single laser-illuminated point-source and single point-detector (which are scanned in tandem across the object) or white-light illumination with multiple point-sources and detectors. Single-point-source systems, however, do not usually form images in real time and are restricted to using available laser wavelengths. Multiple-point-source systems, on the other hand, produce images in real time but use light very inefficiently - typically 1% or less is used for imaging. Here we demonstrate a white-light, multiple-point-source method which can in principle produce images in real time with light efficiencies as high as 50%. This system is likely to find broad practical application, particularly in the imaging of weakly reflecting or weakly fluorescent specimens.
ER -
JUŠKAITIS, Rimas, Tony WILSON, Mark NEIL a Michal KOZUBEK. Efficient real-time confocal microscopy with white light sources. \textit{Nature}. 1996, roč.~383, č.~6603, s.~804-806. ISSN~0028-0836.
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