Intact ribosomal DNA arrays of Potentilla origin detected in
Erythronium nucleus suggest recent eudicot-to-monocot
horizontal transfer

J 2022

Intact ribosomal DNA arrays of Potentilla origin detected in Erythronium nucleus suggest recent eudicot-to-monocot horizontal transfer

BARTHA, Laszlo, Terezie MALÍK MANDÁKOVÁ, Ales KOVARIK, Paul-Adrian BULZU, Nathalie RODDE et. al.

Základní údaje

Originální název

Intact ribosomal DNA arrays of Potentilla origin detected in Erythronium nucleus suggest recent eudicot-to-monocot horizontal transfer

Autoři

BARTHA, Laszlo, Terezie MALÍK MANDÁKOVÁ (203 Česká republika, domácí), Ales KOVARIK, Paul-Adrian BULZU, Nathalie RODDE, Vaclav MAHELKA, Martin LYSÁK (203 Česká republika, garant, domácí), Margaux-Alison FUSTIER, Jan SAFAR, Petr CAPAL, Lujza KERESZTES a Horia L BANCIU

Vydání

New Phytologist, Blackwell Science, 2022, 0028-646X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 9.400

Kód RIV

RIV/00216224:14740/22:00128173

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1111/nph.18171

UT WoS

000798873000001

Klíčová slova anglicky

Erythronium; horizontal gene transfer; internal transcribed spacer; lateral gene transfer; Potentilla; rDNA; ribosomal RNA genes

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 1. 2023 13:44, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

During our initial phylogenetic study of the monocot genus Erythronium (Liliaceae), we observed peculiar eudicot-type internal transcribed spacer (ITS) sequences in a dataset derived from genomic DNA of Erythronium dens-canis. This raised the possibility of horizontal transfer of a eudicot alien ribosomal DNA (rDNA) into the Erythronium genome. In this work we aimed to support this hypothesis by carrying out genomic, molecular, and cytogenetic analyses. Genome skimming coupled by PacBio HiFi sequencing of a bacterial artificial chromosome clone derived from flow-sorted nuclei was used to characterise the alien 45S rDNA. Integration of alien rDNA in the recipient genome was further proved by Southern blotting and fluorescence in situ hybridization using specific probes. Alien rDNA, nested among Potentilla species in phylogenetic analysis, likely entered the Erythronium lineage in the common ancestor of E. dens-canis and E. caucasicum. Transferred eudicot-type rDNA preserved its tandemly arrayed feature on a single chromosome and was found to be transcribed in the monocot host, albeit much less efficiently than the native counterpart. This study adds a new example to the rarely documented nuclear-to-nuclear jumps of DNA between eudicots and monocots while holding the scientific community continually in suspense about the mode of DNA transfer.

Citovat

BARTHA, Laszlo, Terezie MALÍK MANDÁKOVÁ, Ales KOVARIK, Paul-Adrian BULZU, Nathalie RODDE, Vaclav MAHELKA, Martin LYSÁK, Margaux-Alison FUSTIER, Jan SAFAR, Petr CAPAL, Lujza KERESZTES a Horia L BANCIU. Intact ribosomal DNA arrays of Potentilla origin detected in Erythronium nucleus suggest recent eudicot-to-monocot horizontal transfer. New Phytologist. Blackwell Science, 2022, roč. 235, č. 3, s. 1246-1259. ISSN 0028-646X. Dostupné z: https://dx.doi.org/10.1111/nph.18171.

@article{2248077,
   author = {Bartha, Laszlo and Malík Mandáková, Terezie and Kovarik, Ales and Bulzu, PaulandAdrian and Rodde, Nathalie and Mahelka, Vaclav and Lysák, Martin and Fustier, MargauxandAlison and Safar, Jan and Capal, Petr and Keresztes, Lujza and Banciu, Horia L},
   article_number = {3},
   doi = {http://dx.doi.org/10.1111/nph.18171},
   keywords = {Erythronium; horizontal gene transfer; internal transcribed spacer; lateral gene transfer; Potentilla; rDNA; ribosomal RNA genes},
   language = {eng},
   issn = {0028-646X},
   journal = {New Phytologist},
   title = {Intact ribosomal DNA arrays of Potentilla origin detected in Erythronium nucleus suggest recent eudicot-to-monocot horizontal transfer},
   url = {https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.18171},
   volume = {235},
   year = {2022}
}

TY  - JOUR
ID  - 2248077
AU  - Bartha, Laszlo - Malík Mandáková, Terezie - Kovarik, Ales - Bulzu, Paul-Adrian - Rodde, Nathalie - Mahelka, Vaclav - Lysák, Martin - Fustier, Margaux-Alison - Safar, Jan - Capal, Petr - Keresztes, Lujza - Banciu, Horia L
PY  - 2022
TI  - Intact ribosomal DNA arrays of Potentilla origin detected in Erythronium nucleus suggest recent eudicot-to-monocot horizontal transfer
JF  - New Phytologist
VL  - 235
IS  - 3
SP  - 1246-1259
EP  - 1246-1259
PB  - Blackwell Science
SN  - 0028646X
KW  - Erythronium
KW  - horizontal gene transfer
KW  - internal transcribed spacer
KW  - lateral gene transfer
KW  - Potentilla
KW  - rDNA
KW  - ribosomal RNA genes
UR  - https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.18171
N2  - During our initial phylogenetic study of the monocot genus Erythronium (Liliaceae), we observed peculiar eudicot-type internal transcribed spacer (ITS) sequences in a dataset derived from genomic DNA of Erythronium dens-canis. This raised the possibility of horizontal transfer of a eudicot alien ribosomal DNA (rDNA) into the Erythronium genome. In this work we aimed to support this hypothesis by carrying out genomic, molecular, and cytogenetic analyses. Genome skimming coupled by PacBio HiFi sequencing of a bacterial artificial chromosome clone derived from flow-sorted nuclei was used to characterise the alien 45S rDNA. Integration of alien rDNA in the recipient genome was further proved by Southern blotting and fluorescence in situ hybridization using specific probes. Alien rDNA, nested among Potentilla species in phylogenetic analysis, likely entered the Erythronium lineage in the common ancestor of E. dens-canis and E. caucasicum. Transferred eudicot-type rDNA preserved its tandemly arrayed feature on a single chromosome and was found to be transcribed in the monocot host, albeit much less efficiently than the native counterpart. This study adds a new example to the rarely documented nuclear-to-nuclear jumps of DNA between eudicots and monocots while holding the scientific community continually in suspense about the mode of DNA transfer.
ER  -

BARTHA, Laszlo, Terezie MALÍK MANDÁKOVÁ, Ales KOVARIK, Paul-Adrian BULZU, Nathalie RODDE, Vaclav MAHELKA, Martin LYSÁK, Margaux-Alison FUSTIER, Jan SAFAR, Petr CAPAL, Lujza KERESZTES a Horia L BANCIU. Intact ribosomal DNA arrays of Potentilla origin detected in Erythronium nucleus suggest recent eudicot-to-monocot horizontal transfer. \textit{New Phytologist}. Blackwell Science, 2022, roč.~235, č.~3, s.~1246-1259. ISSN~0028-646X. Dostupné z: https://dx.doi.org/10.1111/nph.18171.

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