Continuous double-strand break induction and their differential
processing sustain chiasma formation during Caenorhabditis
elegans meiosis

J 2022

Continuous double-strand break induction and their differential processing sustain chiasma formation during Caenorhabditis elegans meiosis

HICKS, Tara, Shalini TRIVEDI, Mikayla EPPERT, Richard BOWMAN, Hui TIAN et. al.

Základní údaje

Originální název

Continuous double-strand break induction and their differential processing sustain chiasma formation during Caenorhabditis elegans meiosis

Autoři

HICKS, Tara, Shalini TRIVEDI (356 Indie, domácí), Mikayla EPPERT, Richard BOWMAN, Hui TIAN, Amna DAFALLA, Caroline CRAHAN, Sarit SMOLIKOVE a Nicola SILVA (380 Itálie, garant, domácí)

Vydání

Cell Reports, CAMBRIDGE, Cell Press, 2022, 2211-1247

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 8.800

Kód RIV

RIV/00216224:14110/22:00128181

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.celrep.2022.111403

UT WoS

000869019800008

Klíčová slova anglicky

Caenorhabditis elegans meiosis; double-strand break induction; sustain chiasma formation

Štítky

14110513, CF CELLIM, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 1. 2023 14:10, Mgr. Tereza Miškechová

Anotace

V originále

Faithful chromosome segregation into gametes depends on Spo11-induced DNA double-strand breaks (DSBs). These yield single-stranded 30 tails upon resection to promote crossovers (COs). While early Mre11-dependent end resection is the predominant pathway in most organisms, Exo1 or Dna2/BLM can also contribute to the efficient processing of meiotic DSBs. Although its enzymatic activity has been thor-oughly dissected, the temporal dynamics underlying Spo11 activity have remained mostly elusive. We show that, in Caenorhabditis elegans, SPO-11-mediated DSB induction takes place throughout early meiotic prophase I until mid-late pachynema. We find that late DSBs are essential for CO formation and are prefer-entially processed by EXO-1 and DNA-2 in a redundant fashion. Further, EXO-1-DNA-2-mediated resection ensures completion of conservative DSB repair and discourages activation of KU-dependent end joining. Taken together, our data unveil important temporal aspects of DSB induction and identify previously un-known functional implications for EXO-1-DNA-2-mediated resection activity in C. elegans.

Návaznosti

GA20-08819S, projekt VaV

Název: Pochopení úlohy PARG při podpoře tvorby a oprav dvouřetězcových zlomů DNA v meióze

Investor: Grantová agentura ČR, Pochopení úlohy PARG při podpoře tvorby a oprav dvouřetězcových zlomů DNA v meióze

LM2018129, projekt VaV

Název: Národní infrastruktura pro biologické a medicínské zobrazování Czech-BioImaging

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, National research infrastructure for biological and medical imaging

Citovat

HICKS, Tara, Shalini TRIVEDI, Mikayla EPPERT, Richard BOWMAN, Hui TIAN, Amna DAFALLA, Caroline CRAHAN, Sarit SMOLIKOVE a Nicola SILVA. Continuous double-strand break induction and their differential processing sustain chiasma formation during Caenorhabditis elegans meiosis. Cell Reports. CAMBRIDGE: Cell Press, 2022, roč. 40, č. 13, s. 1-20. ISSN 2211-1247. Dostupné z: https://dx.doi.org/10.1016/j.celrep.2022.111403.

@article{2248097,
   author = {Hicks, Tara and Trivedi, Shalini and Eppert, Mikayla and Bowman, Richard and Tian, Hui and Dafalla, Amna and Crahan, Caroline and Smolikove, Sarit and Silva, Nicola},
   article_location = {CAMBRIDGE},
   article_number = {13},
   doi = {http://dx.doi.org/10.1016/j.celrep.2022.111403},
   keywords = {Caenorhabditis elegans meiosis; double-strand break induction; sustain chiasma formation},
   language = {eng},
   issn = {2211-1247},
   journal = {Cell Reports},
   title = {Continuous double-strand break induction and their differential processing sustain chiasma formation during Caenorhabditis elegans meiosis},
   url = {https://www.sciencedirect.com/science/article/pii/S2211124722012402?via%3Dihub},
   volume = {40},
   year = {2022}
}

TY  - JOUR
ID  - 2248097
AU  - Hicks, Tara - Trivedi, Shalini - Eppert, Mikayla - Bowman, Richard - Tian, Hui - Dafalla, Amna - Crahan, Caroline - Smolikove, Sarit - Silva, Nicola
PY  - 2022
TI  - Continuous double-strand break induction and their differential processing sustain chiasma formation during Caenorhabditis elegans meiosis
JF  - Cell Reports
VL  - 40
IS  - 13
SP  - 1-20
EP  - 1-20
PB  - Cell Press
SN  - 22111247
KW  - Caenorhabditis elegans meiosis
KW  - double-strand break induction
KW  - sustain chiasma formation
UR  - https://www.sciencedirect.com/science/article/pii/S2211124722012402?via%3Dihub
N2  - Faithful chromosome segregation into gametes depends on Spo11-induced DNA double-strand breaks (DSBs). These yield single-stranded 30 tails upon resection to promote crossovers (COs). While early Mre11-dependent end resection is the predominant pathway in most organisms, Exo1 or Dna2/BLM can also contribute to the efficient processing of meiotic DSBs. Although its enzymatic activity has been thor-oughly dissected, the temporal dynamics underlying Spo11 activity have remained mostly elusive. We show that, in Caenorhabditis elegans, SPO-11-mediated DSB induction takes place throughout early meiotic prophase I until mid-late pachynema. We find that late DSBs are essential for CO formation and are prefer-entially processed by EXO-1 and DNA-2 in a redundant fashion. Further, EXO-1-DNA-2-mediated resection ensures completion of conservative DSB repair and discourages activation of KU-dependent end joining. Taken together, our data unveil important temporal aspects of DSB induction and identify previously un-known functional implications for EXO-1-DNA-2-mediated resection activity in C. elegans.
ER  -

HICKS, Tara, Shalini TRIVEDI, Mikayla EPPERT, Richard BOWMAN, Hui TIAN, Amna DAFALLA, Caroline CRAHAN, Sarit SMOLIKOVE a Nicola SILVA. Continuous double-strand break induction and their differential processing sustain chiasma formation during Caenorhabditis elegans meiosis. \textit{Cell Reports}. CAMBRIDGE: Cell Press, 2022, roč.~40, č.~13, s.~1-20. ISSN~2211-1247. Dostupné z: https://dx.doi.org/10.1016/j.celrep.2022.111403.

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