Continuous double-strand break induction and their differential
processing sustain chiasma formation during Caenorhabditis
elegans meiosis

J 2022

Continuous double-strand break induction and their differential processing sustain chiasma formation during Caenorhabditis elegans meiosis

HICKS, Tara; Shalini TRIVEDI; Mikayla EPPERT; Richard BOWMAN; Hui TIAN et. al.

Základní údaje

Originální název

Continuous double-strand break induction and their differential processing sustain chiasma formation during Caenorhabditis elegans meiosis

Autoři

HICKS, Tara; Shalini TRIVEDI (356 Indie, domácí); Mikayla EPPERT; Richard BOWMAN; Hui TIAN; Amna DAFALLA; Caroline CRAHAN; Sarit SMOLIKOVE a Nicola SILVA (380 Itálie, garant, domácí)

Vydání

Cell Reports, CAMBRIDGE, Cell Press, 2022, 2211-1247

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 8.800

Kód RIV

RIV/00216224:14110/22:00128181

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1016/j.celrep.2022.111403

UT WoS

000869019800008

EID Scopus

2-s2.0-85138811143

Klíčová slova anglicky

Caenorhabditis elegans meiosis; double-strand break induction; sustain chiasma formation

Štítky

14110513, CF CELLIM, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 5. 2025 13:20, Mgr. Tereza Miškechová

Anotace

V originále

Faithful chromosome segregation into gametes depends on Spo11-induced DNA double-strand breaks (DSBs). These yield single-stranded 30 tails upon resection to promote crossovers (COs). While early Mre11-dependent end resection is the predominant pathway in most organisms, Exo1 or Dna2/BLM can also contribute to the efficient processing of meiotic DSBs. Although its enzymatic activity has been thor-oughly dissected, the temporal dynamics underlying Spo11 activity have remained mostly elusive. We show that, in Caenorhabditis elegans, SPO-11-mediated DSB induction takes place throughout early meiotic prophase I until mid-late pachynema. We find that late DSBs are essential for CO formation and are prefer-entially processed by EXO-1 and DNA-2 in a redundant fashion. Further, EXO-1-DNA-2-mediated resection ensures completion of conservative DSB repair and discourages activation of KU-dependent end joining. Taken together, our data unveil important temporal aspects of DSB induction and identify previously un-known functional implications for EXO-1-DNA-2-mediated resection activity in C. elegans.

Návaznosti

GA20-08819S, projekt VaV

Název: Pochopení úlohy PARG při podpoře tvorby a oprav dvouřetězcových zlomů DNA v meióze

Investor: Grantová agentura ČR, Pochopení úlohy PARG při podpoře tvorby a oprav dvouřetězcových zlomů DNA v meióze

90129, velká výzkumná infrastruktura

Název: Czech-BioImaging II

Citovat

HICKS, Tara; Shalini TRIVEDI; Mikayla EPPERT; Richard BOWMAN; Hui TIAN; Amna DAFALLA; Caroline CRAHAN; Sarit SMOLIKOVE a Nicola SILVA. Continuous double-strand break induction and their differential processing sustain chiasma formation during Caenorhabditis elegans meiosis. Cell Reports. CAMBRIDGE: Cell Press, 2022, roč. 40, č. 13, s. 1-20. ISSN 2211-1247. Dostupné z: https://doi.org/10.1016/j.celrep.2022.111403.

@article{2248097,
   author = {Hicks, Tara and Trivedi, Shalini and Eppert, Mikayla and Bowman, Richard and Tian, Hui and Dafalla, Amna and Crahan, Caroline and Smolikove, Sarit and Silva, Nicola},
   article_location = {CAMBRIDGE},
   article_number = {13},
   doi = {https://doi.org/10.1016/j.celrep.2022.111403},
   keywords = {Caenorhabditis elegans meiosis; double-strand break induction; sustain chiasma formation},
   language = {eng},
   issn = {2211-1247},
   journal = {Cell Reports},
   title = {Continuous double-strand break induction and their differential processing sustain chiasma formation during Caenorhabditis elegans meiosis},
   url = {https://www.sciencedirect.com/science/article/pii/S2211124722012402?via%3Dihub},
   volume = {40},
   year = {2022}
}

TY  - JOUR
ID  - 2248097
AU  - Hicks, Tara - Trivedi, Shalini - Eppert, Mikayla - Bowman, Richard - Tian, Hui - Dafalla, Amna - Crahan, Caroline - Smolikove, Sarit - Silva, Nicola
PY  - 2022
TI  - Continuous double-strand break induction and their differential processing sustain chiasma formation during Caenorhabditis elegans meiosis
JF  - Cell Reports
VL  - 40
IS  - 13
SP  - 1-20
EP  - 1-20
PB  - Cell Press
SN  - 22111247
KW  - Caenorhabditis elegans meiosis
KW  - double-strand break induction
KW  - sustain chiasma formation
UR  - https://www.sciencedirect.com/science/article/pii/S2211124722012402?via%3Dihub
N2  - Faithful chromosome segregation into gametes depends on Spo11-induced DNA double-strand breaks (DSBs). These yield single-stranded 30 tails upon resection to promote crossovers (COs). While early Mre11-dependent end resection is the predominant pathway in most organisms, Exo1 or Dna2/BLM can also contribute to the efficient processing of meiotic DSBs. Although its enzymatic activity has been thor-oughly dissected, the temporal dynamics underlying Spo11 activity have remained mostly elusive. We show that, in Caenorhabditis elegans, SPO-11-mediated DSB induction takes place throughout early meiotic prophase I until mid-late pachynema. We find that late DSBs are essential for CO formation and are prefer-entially processed by EXO-1 and DNA-2 in a redundant fashion. Further, EXO-1-DNA-2-mediated resection ensures completion of conservative DSB repair and discourages activation of KU-dependent end joining. Taken together, our data unveil important temporal aspects of DSB induction and identify previously un-known functional implications for EXO-1-DNA-2-mediated resection activity in C. elegans.
ER  -

HICKS, Tara; Shalini TRIVEDI; Mikayla EPPERT; Richard BOWMAN; Hui TIAN; Amna DAFALLA; Caroline CRAHAN; Sarit SMOLIKOVE a Nicola SILVA. Continuous double-strand break induction and their differential processing sustain chiasma formation during Caenorhabditis elegans meiosis. \textit{Cell Reports}. CAMBRIDGE: Cell Press, 2022, roč.~40, č.~13, s.~1-20. ISSN~2211-1247. Dostupné z: https://doi.org/10.1016/j.celrep.2022.111403.

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