Classification of clinical Cutibacterium acnes isolates at
phylotype level by capillary electrophoretic methods in
roughened fused silica capillary

J 2022

Classification of clinical Cutibacterium acnes isolates at phylotype level by capillary electrophoretic methods in roughened fused silica capillary

HORKA, Marie, Filip RŮŽIČKA, Pavel KARASEK, Jiri SALPLACHTA, Alena SIVÁKOVÁ et. al.

Basic information

Original name

Classification of clinical Cutibacterium acnes isolates at phylotype level by capillary electrophoretic methods in roughened fused silica capillary

Authors

HORKA, Marie (203 Czech Republic), Filip RŮŽIČKA (203 Czech Republic, belonging to the institution), Pavel KARASEK (203 Czech Republic), Jiri SALPLACHTA (203 Czech Republic, guarantor), Alena SIVÁKOVÁ (203 Czech Republic, belonging to the institution) and Michal ROTH (203 Czech Republic)

Edition

Talanta, AMSTERDAM, Elsevier, 2022, 0039-9140

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.100

RIV identification code

RIV/00216224:14110/22:00128305

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.talanta.2022.123565

UT WoS

000812141900002

Keywords in English

Capillary electrophoresis; Classification; Cutibacterium acnes; Fused silica capillary; Phylotype; Supercritical water

Abstract

V originále

Despite being commensal bacterium involved in the maintenance of healthy skin, Cutibacterium acnes is also associated with inflammatory diseases. Since inflammatory and immunogenic properties vary between C. acnes phylotypes, reliable classification of clinical C. acnes isolates is important for determining their pathogenicity. Combination of optimized separation methods, polymer-enhanced transient isotachophoresis and sweeping of the charged bacterial cells in micellar electrokinetic chromatography in the roughened fused silica capillary, was used for the separation of twenty clinical C. acnes isolates. Their correct classification into the individual phylotypes was achieved in 20 min at laboratory temperature. In addition, decrease in the separation temperature to 15 degrees C led to the separation of the individual isolates of some phylotypes. Relative standard deviations of migration times of both intra- and inter-day analyses did not exceed 1.7%. Linearity of the proposed method in the concentration range from 5 x 10(5) to 1 x 10(7) cells mL(-1) was characterized by the coefficient of determination R-2 = 0.9985. Limit of detection of 5 x 10(5) cells mL(-1) (50 cells in 100 nL of the injected sample) was determined for all the examined bacteria.

Citovat

HORKA, Marie, Filip RŮŽIČKA, Pavel KARASEK, Jiri SALPLACHTA, Alena SIVÁKOVÁ and Michal ROTH. Classification of clinical Cutibacterium acnes isolates at phylotype level by capillary electrophoretic methods in roughened fused silica capillary. Talanta. AMSTERDAM: Elsevier, 2022, vol. 247, September 2022, p. 1-7. ISSN 0039-9140. Available from: https://dx.doi.org/10.1016/j.talanta.2022.123565.

@article{2249998,
   author = {Horka, Marie and Růžička, Filip and Karasek, Pavel and Salplachta, Jiri and Siváková, Alena and Roth, Michal},
   article_location = {AMSTERDAM},
   article_number = {September 2022},
   doi = {http://dx.doi.org/10.1016/j.talanta.2022.123565},
   keywords = {Capillary electrophoresis; Classification; Cutibacterium acnes; Fused silica capillary; Phylotype; Supercritical water},
   language = {eng},
   issn = {0039-9140},
   journal = {Talanta},
   title = {Classification of clinical Cutibacterium acnes isolates at phylotype level by capillary electrophoretic methods in roughened fused silica capillary},
   url = {https://www.sciencedirect.com/science/article/pii/S0039914022003617?via%3Dihub},
   volume = {247},
   year = {2022}
}

TY  - JOUR
ID  - 2249998
AU  - Horka, Marie - Růžička, Filip - Karasek, Pavel - Salplachta, Jiri - Siváková, Alena - Roth, Michal
PY  - 2022
TI  - Classification of clinical Cutibacterium acnes isolates at phylotype level by capillary electrophoretic methods in roughened fused silica capillary
JF  - Talanta
VL  - 247
IS  - September 2022
SP  - 1-7
EP  - 1-7
PB  - Elsevier
SN  - 00399140
KW  - Capillary electrophoresis
KW  - Classification
KW  - Cutibacterium acnes
KW  - Fused silica capillary
KW  - Phylotype
KW  - Supercritical water
UR  - https://www.sciencedirect.com/science/article/pii/S0039914022003617?via%3Dihub
N2  - Despite being commensal bacterium involved in the maintenance of healthy skin, Cutibacterium acnes is also associated with inflammatory diseases. Since inflammatory and immunogenic properties vary between C. acnes phylotypes, reliable classification of clinical C. acnes isolates is important for determining their pathogenicity. Combination of optimized separation methods, polymer-enhanced transient isotachophoresis and sweeping of the charged bacterial cells in micellar electrokinetic chromatography in the roughened fused silica capillary, was used for the separation of twenty clinical C. acnes isolates. Their correct classification into the individual phylotypes was achieved in 20 min at laboratory temperature. In addition, decrease in the separation temperature to 15 degrees C led to the separation of the individual isolates of some phylotypes. Relative standard deviations of migration times of both intra- and inter-day analyses did not exceed 1.7%. Linearity of the proposed method in the concentration range from 5 x 10(5) to 1 x 10(7) cells mL(-1) was characterized by the coefficient of determination R-2 = 0.9985. Limit of detection of 5 x 10(5) cells mL(-1) (50 cells in 100 nL of the injected sample) was determined for all the examined bacteria.
ER  -

HORKA, Marie, Filip RŮŽIČKA, Pavel KARASEK, Jiri SALPLACHTA, Alena SIVÁKOVÁ and Michal ROTH. Classification of clinical Cutibacterium acnes isolates at phylotype level by capillary electrophoretic methods in roughened fused silica capillary. \textit{Talanta}. AMSTERDAM: Elsevier, 2022, vol.~247, September 2022, p.~1-7. ISSN~0039-9140. Available from: https://dx.doi.org/10.1016/j.talanta.2022.123565.

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