High frequency of Nichols-like strains and increased levels of
macrolide resistance in Treponema pallidum in clinical samples
from Buenos Aires, Argentina

J 2022

High frequency of Nichols-like strains and increased levels of macrolide resistance in Treponema pallidum in clinical samples from Buenos Aires, Argentina

MORANDO, Nicolas, Eliška VRBOVÁ, Asunta MELGAR, Roberto Daniel RABINOVICH, David ŠMAJS et. al.

Základní údaje

Originální název

High frequency of Nichols-like strains and increased levels of macrolide resistance in Treponema pallidum in clinical samples from Buenos Aires, Argentina

Autoři

MORANDO, Nicolas, Eliška VRBOVÁ (203 Česká republika, domácí), Asunta MELGAR, Roberto Daniel RABINOVICH, David ŠMAJS (203 Česká republika, domácí) a Maria A PANDO (garant)

Vydání

Nature Scientific Reports, Berlin, NATURE RESEARCH, 2022, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.600

Kód RIV

RIV/00216224:14110/22:00128324

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1038/s41598-022-20410-5

UT WoS

000862059200008

Klíčová slova anglicky

Nichols-like strains; Treponema pallidum; macrolide resistance

Štítky

14110513, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 1. 2023 12:28, Mgr. Tereza Miškechová

Anotace

V originále

Globally, 94% of Treponema pallidum subsp. pallidum (TPA) clinical strains belong to the SS14-like group and 6% to the Nichols-like group, with a prevalence of macrolide resistance of 90%. Our goal was to determine whether local TPA strain distribution and macrolide resistance frequency have changed significantly since our last report, which revealed that Buenos Aires had a high frequency of Nichols-like strains (27%) and low levels of macrolide resistance (14%). Swab samples from patients with suspected syphilis were collected during 2015-2019 and loci TP0136, TP0548, TP0705 were sequenced in order to perform multilocus sequence typing. Strains were classified as Nichols-like or SS14-like. The presence of macrolide resistance-associated mutations was determined by examination of the 23S rDNA gene sequence. Of 46 typeable samples, 37% were classified as Nichols-like and 63% as SS14-like. Macrolide resistance prevalence was 45.7%. Seven allelic profiles were found, five were SS14-like and two were Nichols-like. The frequency of Nichols-like strains increased between studies (26.8% vs. 37%, p = 0.36). A dramatic increase was found in the frequency of macrolide resistant strains between studies (14.3% vs. 45.7%, p = 0.005). Our results are in agreement with international trends and underscore the need to pursue further TPA molecular typing studies in South America.

Citovat

MORANDO, Nicolas, Eliška VRBOVÁ, Asunta MELGAR, Roberto Daniel RABINOVICH, David ŠMAJS a Maria A PANDO. High frequency of Nichols-like strains and increased levels of macrolide resistance in Treponema pallidum in clinical samples from Buenos Aires, Argentina. Nature Scientific Reports. Berlin: NATURE RESEARCH, 2022, roč. 12, č. 1, s. 1-10. ISSN 2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-022-20410-5.

@article{2250049,
   author = {Morando, Nicolas and Vrbová, Eliška and Melgar, Asunta and Rabinovich, Roberto Daniel and Šmajs, David and Pando, Maria A},
   article_location = {Berlin},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-022-20410-5},
   keywords = {Nichols-like strains; Treponema pallidum; macrolide resistance},
   language = {eng},
   issn = {2045-2322},
   journal = {Nature Scientific Reports},
   title = {High frequency of Nichols-like strains and increased levels of macrolide resistance in Treponema pallidum in clinical samples from Buenos Aires, Argentina},
   url = {https://www.nature.com/articles/s41598-022-20410-5},
   volume = {12},
   year = {2022}
}

TY  - JOUR
ID  - 2250049
AU  - Morando, Nicolas - Vrbová, Eliška - Melgar, Asunta - Rabinovich, Roberto Daniel - Šmajs, David - Pando, Maria A
PY  - 2022
TI  - High frequency of Nichols-like strains and increased levels of macrolide resistance in Treponema pallidum in clinical samples from Buenos Aires, Argentina
JF  - Nature Scientific Reports
VL  - 12
IS  - 1
SP  - 1-10
EP  - 1-10
PB  - NATURE RESEARCH
SN  - 20452322
KW  - Nichols-like strains
KW  - Treponema pallidum
KW  - macrolide resistance
UR  - https://www.nature.com/articles/s41598-022-20410-5
N2  - Globally, 94% of Treponema pallidum subsp. pallidum (TPA) clinical strains belong to the SS14-like group and 6% to the Nichols-like group, with a prevalence of macrolide resistance of 90%. Our goal was to determine whether local TPA strain distribution and macrolide resistance frequency have changed significantly since our last report, which revealed that Buenos Aires had a high frequency of Nichols-like strains (27%) and low levels of macrolide resistance (14%). Swab samples from patients with suspected syphilis were collected during 2015-2019 and loci TP0136, TP0548, TP0705 were sequenced in order to perform multilocus sequence typing. Strains were classified as Nichols-like or SS14-like. The presence of macrolide resistance-associated mutations was determined by examination of the 23S rDNA gene sequence. Of 46 typeable samples, 37% were classified as Nichols-like and 63% as SS14-like. Macrolide resistance prevalence was 45.7%. Seven allelic profiles were found, five were SS14-like and two were Nichols-like. The frequency of Nichols-like strains increased between studies (26.8% vs. 37%, p = 0.36). A dramatic increase was found in the frequency of macrolide resistant strains between studies (14.3% vs. 45.7%, p = 0.005). Our results are in agreement with international trends and underscore the need to pursue further TPA molecular typing studies in South America.
ER  -

MORANDO, Nicolas, Eliška VRBOVÁ, Asunta MELGAR, Roberto Daniel RABINOVICH, David ŠMAJS a Maria A PANDO. High frequency of Nichols-like strains and increased levels of macrolide resistance in Treponema pallidum in clinical samples from Buenos Aires, Argentina. \textit{Nature Scientific Reports}. Berlin: NATURE RESEARCH, 2022, roč.~12, č.~1, s.~1-10. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-022-20410-5.

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