Prevalence and detection of Helicobacter pylori in raw cows'
and goats' milk in selected farms in the Czech Republic

J 2022

Prevalence and detection of Helicobacter pylori in raw cows' and goats' milk in selected farms in the Czech Republic

FURMANCIKOVA, Petra, Zora STASTKOVA, Pavlina NAVRATILOVA, Ivana BEDNAROVA, Milada DVOŘÁČKOVÁ et. al.

Základní údaje

Originální název

Prevalence and detection of Helicobacter pylori in raw cows' and goats' milk in selected farms in the Czech Republic

Autoři

FURMANCIKOVA, Petra (203 Česká republika, garant), Zora STASTKOVA (203 Česká republika), Pavlina NAVRATILOVA (203 Česká republika), Ivana BEDNAROVA (203 Česká republika) a Milada DVOŘÁČKOVÁ (203 Česká republika, domácí)

Vydání

Journal of Food and Nutrition Research, Bratislava, VUP FOOD RESEARCH INST, 2022, 1336-8672

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.100

Kód RIV

RIV/00216224:14110/22:00128355

Organizační jednotka

Lékařská fakulta

UT WoS

000821448700009

Klíčová slova anglicky

Helicobacter pylori; nested polymerase chain reaction; food safety; raw milk

Štítky

14110113, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 30. 1. 2023 12:44, Mgr. Tereza Miškechová

Anotace

V originále

Helicobacter pylori is currently found in more than a half of the human population and is the cause of serious gastrointestinal diseases such as gastritis, gastric ulcers or adenocarcinoma. H. pylori infection can be transmitted to humans through contaminated raw materials and food of animal origin. In this study, individual cows' milk samples (n = 77) and individual goats' milk samples (n = 52) from two farms in Moravia (Czech Republic) were analysed. The classical culture method and the nested polymerase chain reaction (nested PCR) method were used for detection of H. pylori. The culture method did not demonstrate the presence of H. pylori in any of the examined samples. Using the nested PCR method, 31 samples (40 %) of raw cows' milk and 30 samples (58 %) of raw goats' milk were positive for the presence of H. pylori. The study demonstrated that nested-PCR was highly sensitive for the detection of H. pylori in raw milk samples. The results of the study showed the presence of H. pylori DNA in a high percentage of both cows' and goats' raw milk samples, indicating a high possibility of inter-animal transmission within individual farms.

Citovat

FURMANCIKOVA, Petra, Zora STASTKOVA, Pavlina NAVRATILOVA, Ivana BEDNAROVA a Milada DVOŘÁČKOVÁ. Prevalence and detection of Helicobacter pylori in raw cows' and goats' milk in selected farms in the Czech Republic. Journal of Food and Nutrition Research. Bratislava: VUP FOOD RESEARCH INST, 2022, roč. 61, č. 2, s. 192-197. ISSN 1336-8672.

@article{2250972,
   author = {Furmancikova, Petra and Stastkova, Zora and Navratilova, Pavlina and Bednarova, Ivana and Dvořáčková, Milada},
   article_location = {Bratislava},
   article_number = {2},
   keywords = {Helicobacter pylori; nested polymerase chain reaction; food safety; raw milk},
   language = {eng},
   issn = {1336-8672},
   journal = {Journal of Food and Nutrition Research},
   title = {Prevalence and detection of Helicobacter pylori in raw cows' and goats' milk in selected farms in the Czech Republic},
   url = {https://vup.sk/en/index.php?mainID=2&navID=34&version=2&volume=61&article=2271},
   volume = {61},
   year = {2022}
}

TY  - JOUR
ID  - 2250972
AU  - Furmancikova, Petra - Stastkova, Zora - Navratilova, Pavlina - Bednarova, Ivana - Dvořáčková, Milada
PY  - 2022
TI  - Prevalence and detection of Helicobacter pylori in raw cows' and goats' milk in selected farms in the Czech Republic
JF  - Journal of Food and Nutrition Research
VL  - 61
IS  - 2
SP  - 192-197
EP  - 192-197
PB  - VUP FOOD RESEARCH INST
SN  - 13368672
KW  - Helicobacter pylori
KW  - nested polymerase chain reaction
KW  - food safety
KW  - raw milk
UR  - https://vup.sk/en/index.php?mainID=2&navID=34&version=2&volume=61&article=2271
N2  - Helicobacter pylori is currently found in more than a half of the human population and is the cause of serious gastrointestinal diseases such as gastritis, gastric ulcers or adenocarcinoma. H. pylori infection can be transmitted to humans through contaminated raw materials and food of animal origin. In this study, individual cows' milk samples (n = 77) and individual goats' milk samples (n = 52) from two farms in Moravia (Czech Republic) were analysed. The classical culture method and the nested polymerase chain reaction (nested PCR) method were used for detection of H. pylori. The culture method did not demonstrate the presence of H. pylori in any of the examined samples. Using the nested PCR method, 31 samples (40 %) of raw cows' milk and 30 samples (58 %) of raw goats' milk were positive for the presence of H. pylori. The study demonstrated that nested-PCR was highly sensitive for the detection of H. pylori in raw milk samples. The results of the study showed the presence of H. pylori DNA in a high percentage of both cows' and goats' raw milk samples, indicating a high possibility of inter-animal transmission within individual farms.
ER  -

FURMANCIKOVA, Petra, Zora STASTKOVA, Pavlina NAVRATILOVA, Ivana BEDNAROVA a Milada DVOŘÁČKOVÁ. Prevalence and detection of Helicobacter pylori in raw cows' and goats' milk in selected farms in the Czech Republic. \textit{Journal of Food and Nutrition Research}. Bratislava: VUP FOOD RESEARCH INST, 2022, roč.~61, č.~2, s.~192-197. ISSN~1336-8672.

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