Scale-up of biomass production by Methanococcus maripaludis

@article{2251371,
   author = {Palabikyan, Hayk and Ruddyard, Aquilla and Pomper, Lara and Novák, David and Reischl, Barbara and Rittmann, Simon K.andM.},
   article_number = {November},
   doi = {http://dx.doi.org/10.3389/fmicb.2022.1031131},
   keywords = {archaea biotechnology; bioreactor; bioprocess; fed-batch; anaerobe; methanogen},
   language = {eng},
   issn = {1664-302X},
   journal = {Frontiers in Microbiology},
   title = {Scale-up of biomass production by Methanococcus maripaludis},
   url = {https://doi.org/10.3389/fmicb.2022.1031131},
   volume = {13},
   year = {2022}
}

TY  - JOUR
ID  - 2251371
AU  - Palabikyan, Hayk - Ruddyard, Aquilla - Pomper, Lara - Novák, David - Reischl, Barbara - Rittmann, Simon K.-M.
PY  - 2022
TI  - Scale-up of biomass production by Methanococcus maripaludis
JF  - Frontiers in Microbiology
VL  - 13
IS  - November
SP  - 1-15
EP  - 1-15
PB  - Frontiers Media S.A.
SN  - 1664302X
KW  - archaea biotechnology
KW  - bioreactor
KW  - bioprocess
KW  - fed-batch
KW  - anaerobe
KW  - methanogen
UR  - https://doi.org/10.3389/fmicb.2022.1031131
N2  - The development of a sustainable energy economy is one of the great challenges in the current times of climate crisis and growing energy demands. Industrial production of the fifth-generation biofuel methane by microorganisms has the potential to become a crucial biotechnological milestone of the post fossil fuel era. Therefore, reproducible cultivation and scale-up of methanogenic archaea (methanogens) is essential for enabling biomass generation for fundamental studies and for defining peak performance conditions for bioprocess development. This study provides a comprehensive revision of established and optimization of novel methods for the cultivation of the model organism Methanococcus maripaludis S0001. In closed batch mode, 0.05 L serum bottles cultures were gradually replaced by 0.4 L Schott bottle cultures for regular biomass generation, and the time for reaching peak optical density (OD578) values was reduced in half. In 1.5 L reactor cultures, various agitation, harvesting and transfer methods were compared resulting in a specific growth rate of 0.16 h−1 and the highest recorded OD578 of 3.4. Finally, a 300-fold scale-up from serum bottles was achieved by growing M. maripaludis for the first time in a 22 L stainless steel bioreactor with 15 L working volume. Altogether, the experimental approaches described in this study contribute to establishing methanogens as essential organisms in large-scale biotechnology applications, a crucial stage of an urgently needed industrial evolution toward sustainable biosynthesis of energy and high value products.
ER  - 

PALABIKYAN, Hayk, Aquilla RUDDYARD, Lara POMPER, David NOVÁK, Barbara REISCHL a Simon K.-M. RITTMANN. Scale-up of biomass production by Methanococcus maripaludis. \textit{Frontiers in Microbiology}. Frontiers Media S.A., 2022, roč.~13, November, s.~1-15. ISSN~1664-302X. Dostupné z: https://dx.doi.org/10.3389/fmicb.2022.1031131.