Honeybee Iflaviruses Pack Specific tRNA Fragments from Host
Cells in Their Virions

J 2022

Honeybee Iflaviruses Pack Specific tRNA Fragments from Host Cells in Their Virions

SIMONOVA, Anna; Veronika ROMANSKA; Barbora BENONI; Karel ŠKUBNÍK; Lenka ŠMERDOVÁ et. al.

Základní údaje

Originální název

Honeybee Iflaviruses Pack Specific tRNA Fragments from Host Cells in Their Virions

Autoři

SIMONOVA, Anna; Veronika ROMANSKA; Barbora BENONI; Karel ŠKUBNÍK (203 Česká republika, domácí); Lenka ŠMERDOVÁ (203 Česká republika, domácí); Michaela PROCHÁZKOVÁ (203 Česká republika, domácí); Kristina SPUSTOVA; Ondrej MORAVCIK; Lenka GAHUROVA; Jan PACES; Pavel PLEVKA (203 Česká republika, garant, domácí) a Hana CAHOVA

Vydání

Chembiochem, WEINHEIM, WILEY-VCH, 2022, 1439-4227

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.200

Kód RIV

RIV/00216224:14740/22:00128475

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1002/cbic.202200281

UT WoS

000828265300001

EID Scopus

2-s2.0-85134532067

Klíčová slova anglicky

human rhinovirus type 2; human Echovirus 18; LC-MS; Picornavirales; RNA methylation; tRNA fragments

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 10. 2024 10:12, Ing. Monika Szurmanová, Ph.D.

Anotace

V originále

The Picornavirales include viruses that infect vertebrates, insects, and plants. It was believed that they pack only their genomic mRNA in the particles; thus, we envisaged these viruses as excellent model systems for studies of mRNA modifications. We used LC-MS to analyze digested RNA isolated from particles of the sacbrood and deformed wing iflaviruses as well as of the echovirus 18 and rhinovirus 2 picornaviruses. Whereas in the picornavirus RNAs we detected only N-6-methyladenosine and 2'-O-methylated nucleosides, the iflavirus RNAs contained a wide range of methylated nucleosides, such as 1-methyladenosine (m(1)A) and 5-methylcytidine (m(5)C). Mapping of m(1)A and m(5)C through RNA sequencing of the SBV and DWV RNAs revealed the presence of tRNA molecules. Both modifications were detected only in tRNA. Further analysis revealed that tRNAs are present in form of 3' and 5' fragments and they are packed selectively. Moreover, these tRNAs are typically packed by other viruses.

Návaznosti

90131, velká výzkumná infrastruktura

Název: ELIXIR-CZ II

Citovat

SIMONOVA, Anna; Veronika ROMANSKA; Barbora BENONI; Karel ŠKUBNÍK; Lenka ŠMERDOVÁ; Michaela PROCHÁZKOVÁ; Kristina SPUSTOVA; Ondrej MORAVCIK; Lenka GAHUROVA; Jan PACES; Pavel PLEVKA a Hana CAHOVA. Honeybee Iflaviruses Pack Specific tRNA Fragments from Host Cells in Their Virions. Chembiochem. WEINHEIM: WILEY-VCH, 2022, roč. 23, č. 17, s. 202200281-202200289. ISSN 1439-4227. Dostupné z: https://dx.doi.org/10.1002/cbic.202200281.

@article{2251923,
   author = {Simonova, Anna and Romanska, Veronika and Benoni, Barbora and Škubník, Karel and Šmerdová, Lenka and Procházková, Michaela and Spustova, Kristina and Moravcik, Ondrej and Gahurova, Lenka and Paces, Jan and Plevka, Pavel and Cahova, Hana},
   article_location = {WEINHEIM},
   article_number = {17},
   doi = {http://dx.doi.org/10.1002/cbic.202200281},
   keywords = {human rhinovirus type 2; human Echovirus 18; LC-MS; Picornavirales; RNA methylation; tRNA fragments},
   language = {eng},
   issn = {1439-4227},
   journal = {Chembiochem},
   title = {Honeybee Iflaviruses Pack Specific tRNA Fragments from Host Cells in Their Virions},
   url = {https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cbic.202200281},
   volume = {23},
   year = {2022}
}

TY  - JOUR
ID  - 2251923
AU  - Simonova, Anna - Romanska, Veronika - Benoni, Barbora - Škubník, Karel - Šmerdová, Lenka - Procházková, Michaela - Spustova, Kristina - Moravcik, Ondrej - Gahurova, Lenka - Paces, Jan - Plevka, Pavel - Cahova, Hana
PY  - 2022
TI  - Honeybee Iflaviruses Pack Specific tRNA Fragments from Host Cells in Their Virions
JF  - Chembiochem
VL  - 23
IS  - 17
SP  - 202200281
EP  - 202200281
PB  - WILEY-VCH
SN  - 14394227
KW  - human rhinovirus type 2
KW  - human Echovirus 18
KW  - LC-MS
KW  - Picornavirales
KW  - RNA methylation
KW  - tRNA fragments
UR  - https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cbic.202200281
N2  - The Picornavirales include viruses that infect vertebrates, insects, and plants. It was believed that they pack only their genomic mRNA in the particles; thus, we envisaged these viruses as excellent model systems for studies of mRNA modifications. We used LC-MS to analyze digested RNA isolated from particles of the sacbrood and deformed wing iflaviruses as well as of the echovirus 18 and rhinovirus 2 picornaviruses. Whereas in the picornavirus RNAs we detected only N-6-methyladenosine and 2'-O-methylated nucleosides, the iflavirus RNAs contained a wide range of methylated nucleosides, such as 1-methyladenosine (m(1)A) and 5-methylcytidine (m(5)C). Mapping of m(1)A and m(5)C through RNA sequencing of the SBV and DWV RNAs revealed the presence of tRNA molecules. Both modifications were detected only in tRNA. Further analysis revealed that tRNAs are present in form of 3' and 5' fragments and they are packed selectively. Moreover, these tRNAs are typically packed by other viruses.
ER  -

SIMONOVA, Anna; Veronika ROMANSKA; Barbora BENONI; Karel ŠKUBNÍK; Lenka ŠMERDOVÁ; Michaela PROCHÁZKOVÁ; Kristina SPUSTOVA; Ondrej MORAVCIK; Lenka GAHUROVA; Jan PACES; Pavel PLEVKA a Hana CAHOVA. Honeybee Iflaviruses Pack Specific tRNA Fragments from Host Cells in Their Virions. \textit{Chembiochem}. WEINHEIM: WILEY-VCH, 2022, roč.~23, č.~17, s.~202200281-202200289. ISSN~1439-4227. Dostupné z: https://dx.doi.org/10.1002/cbic.202200281.

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