J 2022

Gut commensal Limosilactobacillus reuteri induces atypical memory-like phenotype in human dendritic cells in vitro

LASAVICIUTE, Gintare, Myriam BARZ, van der Heiden MARIEKE, Claudia ARASA, Kanwal TARIQ et. al.

Základní údaje

Originální název

Gut commensal Limosilactobacillus reuteri induces atypical memory-like phenotype in human dendritic cells in vitro

Autoři

LASAVICIUTE, Gintare, Myriam BARZ, van der Heiden MARIEKE, Claudia ARASA, Kanwal TARIQ, Jaclyn Elizabeth QUIN (36 Austrálie, garant, domácí), Ostlund Farrants ANN-KRISTIN a Eva SVERREMARK-EKSTROM

Vydání

GUT MICROBES, UNITED STATES, TAYLOR & FRANCIS INC, 2022, 1949-0976

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Impakt faktor

Impact factor: 12.200

Kód RIV

RIV/00216224:14740/22:00128508

Organizační jednotka

Středoevropský technologický institut

DOI

UT WoS

000765991300001

Klíčová slova anglicky

Limosilactobacillus reuteri; dendritic cells; T helper cells; innate immune memory; epigenetics

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 2. 2. 2023 16:19, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Memory-like responses in innate immune cells confer nonspecific protection against secondary exposures. A number of microbial agents have been found to induce enhanced or diminished recall responses in innate cells, however, studies investigating the ability of probiotic bacteria to trigger such effects are lacking. Here, we show that priming of human monocytes with a secretome from the gut probiotic bacterium Limosilactobacillus (L.) reuteri induces a mixed secondary response phenotype in monocyte-derived dendritic cells (mo-DCs), with a strong IL-6 and IL-1 beta response but low TNF alpha, IL-23 and IL-27 secretion. Instead, blood DC priming with L. reuteri-secretome resembles a tolerant state upon secondary exposure. A similar pattern was found in conventional and gut-like (retinoic acid exposed) DCs, although retinoic acid hampered TNF alpha and IL-6 production and enrichment of histone modifications in L. reuteri-secretome primed mo-DC cultures. Further, we show that the memory-like phenotype of mo-DCs, induced by priming stimuli, is important for subsequent T helper (Th) cell differentiation pathways and might determine the inflammatory nature of Th cells. We also show enhanced recall responses characterized by robust inflammatory cytokines and lactate production in the gut-like mo-DCs derived from beta-glucan primed monocytes. Such responses were accompanied with enriched histone modifications at the promoter of genes associated with a trained phenotype in myeloid cells. Altogether, we demonstrate that a gut commensal-derived secretome prompts recall responses in human DCs which differ from that induced by classical training agents such as beta-glucan. Our results could be beneficial for future therapeutic interventions where T cell responses are needed to be modulated.