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@article{2252384,
author = {Benešová, M. and Bernatová, S. and Mika, F. and Pokorná, Z. and Ježek, J. and Šiler, M. and Samek, O. and Růžička, Filip and Rebrošová, Katarína and Zemánek, P. and Pilát, Z.},
article_location = {Basel},
article_number = {2},
doi = {http://dx.doi.org/10.3390/bios13020182},
keywords = {SERS-tag; Escherichia coli; sandwich immunoassay; single-cell detection},
language = {eng},
issn = {2079-6374},
journal = {Biosensors},
title = {SERS-Tags: Selective Immobilization and Detection of Bacteria by Strain-Specific Antibodies and Surface-Enhanced Raman Scattering},
url = {https://www.mdpi.com/2079-6374/13/2/182},
volume = {13},
year = {2023}
}
TY - JOUR
ID - 2252384
AU - Benešová, M. - Bernatová, S. - Mika, F. - Pokorná, Z. - Ježek, J. - Šiler, M. - Samek, O. - Růžička, Filip - Rebrošová, Katarína - Zemánek, P. - Pilát, Z.
PY - 2023
TI - SERS-Tags: Selective Immobilization and Detection of Bacteria by Strain-Specific Antibodies and Surface-Enhanced Raman Scattering
JF - Biosensors
VL - 13
IS - 2
SP - 1-14
EP - 1-14
PB - MDPI
SN - 20796374
KW - SERS-tag
KW - Escherichia coli
KW - sandwich immunoassay
KW - single-cell detection
UR - https://www.mdpi.com/2079-6374/13/2/182
N2 - Efficient separation and sensitive identification of pathogenic bacterial strains is essential for a prosperous modern society, with direct applications in medical diagnostics, drug discovery, biodefense, and food safety. We developed a fast and reliable method for antibody-based selective immobilization of bacteria from suspension onto a gold-plated glass surface, followed by detection using strain-specific antibodies linked to gold nanoparticles decorated with a reporter molecule. The reporter molecules are subsequently detected by surface-enhanced Raman spectroscopy (SERS). Such a multi-functionalized nanoparticle is called a SERS-tag. The presented procedure uses widely accessible and cheap materials for manufacturing and functionalization of the nanoparticles and the immobilization surfaces. Here, we exemplify the use of the produced SERS-tags for sensitive single-cell detection of opportunistic pathogen Escherichia coli, and we demonstrate the selectivity of our method using two other bacterial strains, Staphylococcus aureus and Serratia marcescens, as negative controls. We believe that the described approach has a potential to inspire the development of novel medical diagnostic tools for rapid identification of bacterial pathogens.
ER -
BENEŠOVÁ, M., S. BERNATOVÁ, F. MIKA, Z. POKORNÁ, J. JEŽEK, M. ŠILER, O. SAMEK, Filip RŮŽIČKA, Katarína REBROŠOVÁ, P. ZEMÁNEK a Z. PILÁT. SERS-Tags: Selective Immobilization and Detection of Bacteria by Strain-Specific Antibodies and Surface-Enhanced Raman Scattering. \textit{Biosensors}. Basel: MDPI, 2023, roč.~13, č.~2, s.~1-14. ISSN~2079-6374. Dostupné z: https://dx.doi.org/10.3390/bios13020182.
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