Structural and functional basis of mammalian microRNA
biogenesis by Dicer

J 2022

Structural and functional basis of mammalian microRNA biogenesis by Dicer

ZAPLETAL, David, Eliska TABORSKA, Josef PASULKA, Radek MALIK, Karel KUBÍČEK et. al.

Základní údaje

Originální název

Structural and functional basis of mammalian microRNA biogenesis by Dicer

Autoři

ZAPLETAL, David (203 Česká republika, domácí), Eliska TABORSKA, Josef PASULKA, Radek MALIK, Karel KUBÍČEK (203 Česká republika, domácí), Martina ZÁNOVÁ (703 Slovensko, domácí), Christian MUCH, Marek ŠEBESTA (703 Slovensko, domácí), Valeria BUCCHERI, Filip HORVAT, Irena JENICKOVA, Michaela PROCHAZKOVA, Jan PROCHAZKA, Matyáš PINKAS (203 Česká republika, domácí), Jiří NOVÁČEK (203 Česká republika, domácí), Diego F JOSEPH, Radislav SEDLACEK, Carrie BERNECKY, Donal CARROLL, Richard ŠTEFL (203 Česká republika, garant, domácí) a Petr SVOBODA

Vydání

Molecular Cell, CAMBRIDGE, CELL PRESS, 2022, 1097-2765

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 16.000

Kód RIV

RIV/00216224:14740/22:00128561

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.molcel.2022.10.010

UT WoS

000898565300011

Klíčová slova anglicky

CRYO-EM STRUCTUREGUIDE STRAND SELECTIONRNA-BINDINGSTRUCTURE VALIDATIONTRBP COMPLEXMOUSEEXPRESSIONMOLPROBITYSPECIFICITYRECOGNITION

Štítky

CF BioData, CF CRYO, CF PROT, rivok

Změněno: 26. 2. 2023 20:32, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

MicroRNA (miRNA) and RNA interference (RNAi) pathways rely on small RNAs produced by Dicer endonucle-ases. Mammalian Dicer primarily supports the essential gene-regulating miRNA pathway, but how it is spe-cifically adapted to miRNA biogenesis is unknown. We show that the adaptation entails a unique structural role of Dicer???s DExD/H helicase domain. Although mice tolerate loss of its putative ATPase function, the com-plete absence of the domain is lethal because it assures high-fidelity miRNA biogenesis. Structures of murine Dicerd???miRNA precursor complexes revealed that the DExD/H domain has a helicase-unrelated structural function. It locks Dicer in a closed state, which facilitates miRNA precursor selection. Transition to a cleav-age-competent open state is stimulated by Dicer-binding protein TARBP2. Absence of the DExD/H domain or its mutations unlocks the closed state, reduces substrate selectivity, and activates RNAi. Thus, the DExD/H domain structurally contributes to mammalian miRNA biogenesis and underlies mechanistical partitioning of miRNA and RNAi pathways.

Návaznosti

EF19_073/0016943, projekt VaV

Název: Interní grantová agentura Masarykovy univerzity

GA22-19896S, projekt VaV

Název: Strukturní podstata pro opětovné sestavení nukleosomu při přepisu genu zprostředkovaná proteinem Spt6

Investor: Grantová agentura ČR, Strukturní podstata pro opětovné sestavení nukleosomu při přepisu genu zrostředkovaná proteinem Spt6

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LM2018131, projekt VaV

Název: Česká národní infrastruktura pro biologická data (Akronym: ELIXIR-CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Česká národní infrastruktura pro biologická data

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

649030, interní kód MU

Název: DECOR - Dynamic assembly and exchange of RNA polymerase II CTD factors (Akronym: DECOR)

Investor: Evropská unie, DECOR - Dynamic assembly and exchange of RNA polymerase II CTD factors, ERC (Excellent Science)

Citovat

ZAPLETAL, David, Eliska TABORSKA, Josef PASULKA, Radek MALIK, Karel KUBÍČEK, Martina ZÁNOVÁ, Christian MUCH, Marek ŠEBESTA, Valeria BUCCHERI, Filip HORVAT, Irena JENICKOVA, Michaela PROCHAZKOVA, Jan PROCHAZKA, Matyáš PINKAS, Jiří NOVÁČEK, Diego F JOSEPH, Radislav SEDLACEK, Carrie BERNECKY, Donal CARROLL, Richard ŠTEFL a Petr SVOBODA. Structural and functional basis of mammalian microRNA biogenesis by Dicer. Molecular Cell. CAMBRIDGE: CELL PRESS, 2022, roč. 82, č. 21, s. 4064-4079. ISSN 1097-2765. Dostupné z: https://dx.doi.org/10.1016/j.molcel.2022.10.010.

@article{2252813,
   author = {Zapletal, David and Taborska, Eliska and Pasulka, Josef and Malik, Radek and Kubíček, Karel and Zánová, Martina and Much, Christian and Šebesta, Marek and Buccheri, Valeria and Horvat, Filip and Jenickova, Irena and Prochazkova, Michaela and Prochazka, Jan and Pinkas, Matyáš and Nováček, Jiří and Joseph, Diego F and Sedlacek, Radislav and Bernecky, Carrie and Carroll, Donal and Štefl, Richard and Svoboda, Petr},
   article_location = {CAMBRIDGE},
   article_number = {21},
   doi = {http://dx.doi.org/10.1016/j.molcel.2022.10.010},
   keywords = {CRYO-EM STRUCTUREGUIDE STRAND SELECTIONRNA-BINDINGSTRUCTURE VALIDATIONTRBP COMPLEXMOUSEEXPRESSIONMOLPROBITYSPECIFICITYRECOGNITION},
   language = {eng},
   issn = {1097-2765},
   journal = {Molecular Cell},
   title = {Structural and functional basis of mammalian microRNA biogenesis by Dicer},
   url = {https://www.sciencedirect.com/science/article/pii/S1097276522009674?via%3Dihub},
   volume = {82},
   year = {2022}
}

TY  - JOUR
ID  - 2252813
AU  - Zapletal, David - Taborska, Eliska - Pasulka, Josef - Malik, Radek - Kubíček, Karel - Zánová, Martina - Much, Christian - Šebesta, Marek - Buccheri, Valeria - Horvat, Filip - Jenickova, Irena - Prochazkova, Michaela - Prochazka, Jan - Pinkas, Matyáš - Nováček, Jiří - Joseph, Diego F - Sedlacek, Radislav - Bernecky, Carrie - Carroll, Donal - Štefl, Richard - Svoboda, Petr
PY  - 2022
TI  - Structural and functional basis of mammalian microRNA biogenesis by Dicer
JF  - Molecular Cell
VL  - 82
IS  - 21
SP  - 4064-4079
EP  - 4064-4079
PB  - CELL PRESS
SN  - 10972765
KW  - CRYO-EM STRUCTUREGUIDE STRAND SELECTIONRNA-BINDINGSTRUCTURE VALIDATIONTRBP COMPLEXMOUSEEXPRESSIONMOLPROBITYSPECIFICITYRECOGNITION
UR  - https://www.sciencedirect.com/science/article/pii/S1097276522009674?via%3Dihub
N2  - MicroRNA (miRNA) and RNA interference (RNAi) pathways rely on small RNAs produced by Dicer endonucle-ases. Mammalian Dicer primarily supports the essential gene-regulating miRNA pathway, but how it is spe-cifically adapted to miRNA biogenesis is unknown. We show that the adaptation entails a unique structural role of Dicer???s DExD/H helicase domain. Although mice tolerate loss of its putative ATPase function, the com-plete absence of the domain is lethal because it assures high-fidelity miRNA biogenesis. Structures of murine Dicerd???miRNA precursor complexes revealed that the DExD/H domain has a helicase-unrelated structural function. It locks Dicer in a closed state, which facilitates miRNA precursor selection. Transition to a cleav-age-competent open state is stimulated by Dicer-binding protein TARBP2. Absence of the DExD/H domain or its mutations unlocks the closed state, reduces substrate selectivity, and activates RNAi. Thus, the DExD/H domain structurally contributes to mammalian miRNA biogenesis and underlies mechanistical partitioning of miRNA and RNAi pathways.
ER  -

ZAPLETAL, David, Eliska TABORSKA, Josef PASULKA, Radek MALIK, Karel KUBÍČEK, Martina ZÁNOVÁ, Christian MUCH, Marek ŠEBESTA, Valeria BUCCHERI, Filip HORVAT, Irena JENICKOVA, Michaela PROCHAZKOVA, Jan PROCHAZKA, Matyáš PINKAS, Jiří NOVÁČEK, Diego F JOSEPH, Radislav SEDLACEK, Carrie BERNECKY, Donal CARROLL, Richard ŠTEFL a Petr SVOBODA. Structural and functional basis of mammalian microRNA biogenesis by Dicer. \textit{Molecular Cell}. CAMBRIDGE: CELL PRESS, 2022, roč.~82, č.~21, s.~4064-4079. ISSN~1097-2765. Dostupné z: https://dx.doi.org/10.1016/j.molcel.2022.10.010.

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