Pseudomonas aeruginosa bacteriophages isolated from hospital
wastewater and their effect on healthcare-associated strains

a 2022

Pseudomonas aeruginosa bacteriophages isolated from hospital wastewater and their effect on healthcare-associated strains

KOMÁRKOVÁ, Marie, Martin BENEŠÍK, Roman PANTŮČEK, Lucie VALENTOVÁ, Pavel PLEVKA et. al.

Základní údaje

Originální název

Pseudomonas aeruginosa bacteriophages isolated from hospital wastewater and their effect on healthcare-associated strains

Název česky

Bakteriofágy Pseudomonas aeruginosa izolované z nemocničních odpadních vod a jejich efekt na nemocniční kmeny

Autoři

KOMÁRKOVÁ, Marie, Martin BENEŠÍK, Roman PANTŮČEK, Lucie VALENTOVÁ, Pavel PLEVKA, Kateřina PLACHÁ, Kateřina ROSÍKOVÁ, Jana JURČÍKOVÁ a Lucie VALENTOVÁ

Vydání

The Viruses of Microbes 2022, 2022

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Stát vydavatele

Portugalsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

bakteriofágy, Pseudomonas, Česká republika, odpadní vody, nemocniční kmeny

Klíčová slova anglicky

bacteriophages, Pseudomonas, the Czech Republic, wastewater, healthcare-associated strains

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 2. 2023 16:23, Mgr. Marie Komárková

Anotace

V originále

The drug-resistant strains Pseudomonas aeruginosa are the cause of serious and even life-threatening infections. In this case, the application of bacteriophages is hopeful alteration of antibiotic therapy. New phages for therapy could be obtained from environment, when the one of the suitable sources for phage isolation are wastewaters. In this study, we have focused on isolation and characterisation of new bacteriophages with therapeutic potential from wastewater that has same origin (i. e. hospital) as phage host strains of P. aeruginosa. Firstly, we isolated five new pseudomonal bacteriophages from samples of wastewater acquired from UHO (University Hospital Ostrava). We determined the lytic patterns of these phages against 26 UHO isolates of P. aeruginosa together with nineteen pseudomonal phages from FAGOFARMA company collection. Overall the 80.8 % of strains were sensitive (i. e. at least one of the assessed phages formed plaques with these strains). After that, we applied sequencing, PCR, and transmission electron microscopy for phage taxonomic classification. The phages from hospital wastewaters were characterised as members of Pbunavirus and Nankokuvirus genera. According to literature, similar phages are commonly use in commercial therapeutic cocktails. In conclusion, we proved the possible isolation of bacteriophages with therapeutic potential from Czech hospital wastewaters. In the future, these phages can be components of cocktail for treatment of infections caused by drug-resistant strains of P. aeruginosa.

Citovat

KOMÁRKOVÁ, Marie, Martin BENEŠÍK, Roman PANTŮČEK, Lucie VALENTOVÁ, Pavel PLEVKA, Kateřina PLACHÁ, Kateřina ROSÍKOVÁ, Jana JURČÍKOVÁ a Lucie VALENTOVÁ. Pseudomonas aeruginosa bacteriophages isolated from hospital wastewater and their effect on healthcare-associated strains. In The Viruses of Microbes 2022. 2022.

@proceedings{2253795,
   author = {Komárková, Marie and Benešík, Martin and Pantůček, Roman and Valentová, Lucie and Plevka, Pavel and Plachá, Kateřina and Rosíková, Kateřina and Jurčíková, Jana and Valentová, Lucie},
   booktitle = {The Viruses of Microbes 2022},
   keywords = {bacteriophages, Pseudomonas, the Czech Republic, wastewater, healthcare-associated strains},
   language = {eng},
   title = {Pseudomonas aeruginosa bacteriophages isolated from hospital wastewater and their effect on healthcare-associated strains},
   url = {https://skyros-congressos.pt/vom2022/},
   year = {2022}
}

TY  - CONF
ID  - 2253795
AU  - Komárková, Marie - Benešík, Martin - Pantůček, Roman - Valentová, Lucie - Plevka, Pavel - Plachá, Kateřina - Rosíková, Kateřina - Jurčíková, Jana - Valentová, Lucie
PY  - 2022
TI  - Pseudomonas aeruginosa bacteriophages isolated from hospital wastewater and their effect on healthcare-associated strains
KW  - bacteriophages, Pseudomonas, the Czech Republic, wastewater, healthcare-associated strains
UR  - https://skyros-congressos.pt/vom2022/
N2  - The drug-resistant strains Pseudomonas aeruginosa are the cause of serious and even life-threatening infections. In this case, the application of bacteriophages is hopeful alteration of antibiotic therapy. New phages for therapy could be obtained from environment, when the one of the suitable sources for phage isolation are wastewaters. In this study, we have focused on isolation and characterisation of new bacteriophages with therapeutic potential from wastewater that has same origin (i. e. hospital) as phage host strains of P. aeruginosa. Firstly, we isolated five new pseudomonal bacteriophages from samples of wastewater acquired from UHO (University Hospital Ostrava). We determined the lytic patterns of these phages against 26 UHO isolates of P. aeruginosa together with nineteen pseudomonal phages from FAGOFARMA company collection. Overall the 80.8 % of strains were sensitive (i. e. at least one of the assessed phages formed plaques with these strains). After that, we applied sequencing, PCR, and transmission electron microscopy for phage taxonomic classification. The phages from hospital wastewaters were characterised as members of Pbunavirus and Nankokuvirus genera. According to literature, similar phages are commonly use in commercial therapeutic cocktails. In conclusion, we proved the possible isolation of bacteriophages with therapeutic potential from Czech hospital wastewaters. In the future, these phages can be components of cocktail for treatment of infections caused by drug-resistant strains of P. aeruginosa.
ER  -

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