2023
Staphylococcus aureus Prophage-Encoded Protein Causes Abortive Infection and Provides Population Immunity against Kayviruses
KUNTOVÁ, Lucie, Ivana MAŠLAŇOVÁ, Radka OBOŘILOVÁ, Hana ŠIMEČKOVÁ, Adéla FINSTRLOVÁ et. al.Základní údaje
Originální název
Staphylococcus aureus Prophage-Encoded Protein Causes Abortive Infection and Provides Population Immunity against Kayviruses
Autoři
KUNTOVÁ, Lucie (203 Česká republika, domácí), Ivana MAŠLAŇOVÁ (203 Česká republika, domácí), Radka OBOŘILOVÁ (203 Česká republika, domácí), Hana ŠIMEČKOVÁ (203 Česká republika, domácí), Adéla FINSTRLOVÁ (203 Česká republika, domácí), Pavol BÁRDY (703 Slovensko), Marta ŠIBOROVÁ (203 Česká republika, domácí), Liudmyla TROIANOVSKA (804 Ukrajina, domácí), Tibor BOTKA (203 Česká republika, domácí), Petr GINTAR (203 Česká republika, domácí), Ondrej ŠEDO (203 Česká republika, domácí), Zdeněk FARKA (203 Česká republika, domácí), Jiří DOŠKAŘ (203 Česká republika, domácí) a Roman PANTŮČEK (203 Česká republika, garant, domácí)
Vydání
mBio, Washington, DC, USA, American Society for Microbiology, 2023, 2150-7511
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10607 Virology
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 6.400 v roce 2022
Kód RIV
RIV/00216224:14310/23:00130324
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000937378400001
Klíčová slova anglicky
Staphylococcus aureus; lysogeny; phage resistance; abortive infection; Kayvirus; cell death; phage therapy; bacteriophage evolution; bacteriophage therapy; bacteriophages
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 18. 10. 2024 13:07, Ing. Jana Kuchtová
Anotace
V originále
Both temperate and obligately lytic phages have crucial roles in the biology of staphylococci. While superinfection exclusion among closely related temperate phages is a well-characterized phenomenon, the interactions between temperate and lytic phages in staphylococci are not understood. Here, we present a resistance mechanism toward lytic phages of the genus Kayvirus, mediated by the membrane-anchored protein designated PdpSau encoded by Staphylococcus aureus prophages, mostly of the Sa2 integrase type. The prophage accessory gene pdpSau is strongly linked to the lytic genes for holin and ami2-type amidase and typically replaces genes for the toxin Panton-Valentine leukocidin (PVL). The predicted PdpSau protein structure shows the presence of a membrane-binding α-helix in its N-terminal part and a cytoplasmic positively charged C terminus. We demonstrated that the mechanism of action of PdpSau does not prevent the infecting kayvirus from adsorbing onto the host cell and delivering its genome into the cell, but phage DNA replication is halted. Changes in the cell membrane polarity and permeability were observed from 10 min after the infection, which led to prophage-activated cell death. Furthermore, we describe a mechanism of overcoming this resistance in a host-range Kayvirus mutant, which was selected on an S. aureus strain harboring prophage 53 encoding PdpSau, and in which a chimeric gene product emerged via adaptive laboratory evolution. This first case of staphylococcal interfamily phage-phage competition is analogous to some other abortive infection defense systems and to systems based on membrane-destructive proteins. IMPORTANCE. Prophages play an important role in virulence, pathogenesis, and host preference, as well as in horizontal gene transfer in staphylococci. In contrast, broad-host-range lytic staphylococcal kayviruses lyse most S. aureus strains, and scientists worldwide have come to believe that the use of such phages will be successful for treating and preventing bacterial diseases. The effectiveness of phage therapy is complicated by bacterial resistance, whose mechanisms related to therapeutic staphylococcal phages are not understood in detail. In this work, we describe a resistance mechanism targeting kayviruses that is encoded by a prophage. We conclude that the defense mechanism belongs to a broader group of abortive infections, which is characterized by suicidal behavior of infected cells that are unable to produce phage progeny, thus ensuring the survival of the host population. Since the majority of staphylococcal strains are lysogenic, our findings are relevant for the advancement of phage therapy.
Návaznosti
EF18_046/0015974, projekt VaV |
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GA18-13064S, projekt VaV |
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LM2018140, projekt VaV |
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LX22NPO5103, projekt VaV |
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MUNI/A/1325/2021, interní kód MU |
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NU21J-05-00035, projekt VaV |
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NU22-05-00042, projekt VaV |
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90127, velká výzkumná infrastruktura |
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