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@inbook{2260957,
author = {Sumbal, Jakub and Gudjonsson, Thorarinn and Traustadottir, Gunnhildur Asta and Sumbalová Koledová, Zuzana},
address = {New York},
booktitle = {Mammary Stem Cells},
doi = {http://dx.doi.org/10.1007/978-1-0716-2193-6_16},
editor = {Vivanco, M.d. (eds) Mammary Stem Cells},
keywords = {3D culture; Breast; Epithelial-stromal interactions; Fibroblast; Mammary gland; Spheroid.},
howpublished = {tištěná verze "print"},
language = {eng},
location = {New York},
isbn = {978-1-0716-2192-9},
pages = {283-299},
publisher = {Springer New York - Humana Press},
title = {An Organotypic Assay to Study Epithelial-Fibroblast Interactions in Human Breast},
url = {https://link.springer.com/protocol/10.1007/978-1-0716-2193-6_16},
year = {2022}
}
TY - CHAP
ID - 2260957
AU - Sumbal, Jakub - Gudjonsson, Thorarinn - Traustadottir, Gunnhildur Asta - Sumbalová Koledová, Zuzana
PY - 2022
TI - An Organotypic Assay to Study Epithelial-Fibroblast Interactions in Human Breast
VL - Methods in Molecular Biology, vol. 2471
PB - Springer New York - Humana Press
CY - New York
SN - 9781071621929
KW - 3D culture
KW - Breast
KW - Epithelial-stromal interactions
KW - Fibroblast
KW - Mammary gland
KW - Spheroid.
UR - https://link.springer.com/protocol/10.1007/978-1-0716-2193-6_16
N2 - Epithelial–stromal interactions play an essential role in regulation of mammary gland development, homeostasis, and tumorigenesis. Fibroblasts constitute a substantial proportion of mammary gland stromal cells in human breast and have been recognized for their paracrine signaling and extracellular matrix production and remodeling roles during normal breast development as well as in breast cancer. However, our current knowledge on human breast fibroblast functions is incomplete. Here we provide a detailed protocol for an organotypic human breast assay to facilitate research in the roles of human breast fibroblasts in mammary epithelial morphogenesis and early tumorigenesis.
ER -
SUMBAL, Jakub, Thorarinn GUDJONSSON, Gunnhildur Asta TRAUSTADOTTIR and Zuzana SUMBALOVÁ KOLEDOVÁ. An Organotypic Assay to Study Epithelial-Fibroblast Interactions in Human Breast. In Vivanco, M.d. (eds) Mammary Stem Cells. \textit{Mammary Stem Cells}. New York: Springer New York - Humana Press, 2022, p.~283-299. Methods in Molecular Biology, vol. 2471. ISBN~978-1-0716-2192-9. Available from: https://dx.doi.org/10.1007/978-1-0716-2193-6\_{}16.
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