Effects of gonadotropin-releasing hormone agonist administered
in microparticles on sperm quality and quantity, and plasma sex
steroid levels in northern pike

J 2022

Effects of gonadotropin-releasing hormone agonist administered in microparticles on sperm quality and quantity, and plasma sex steroid levels in northern pike

KNOWLES, J., S. BORYSHPOLETS, V. KHOLODNYY, D. RAHI, Jakub VYSLOUŽIL et. al.

Základní údaje

Originální název

Effects of gonadotropin-releasing hormone agonist administered in microparticles on sperm quality and quantity, and plasma sex steroid levels in northern pike

Autoři

KNOWLES, J. (garant), S. BORYSHPOLETS, V. KHOLODNYY, D. RAHI, Jakub VYSLOUŽIL (203 Česká republika, domácí), Jan MUSELÍK (203 Česká republika, domácí), V. STEJSKAL, J. KOURIL a P. PODHOREC

Vydání

ANIMAL, Amsterdam, Elsevier, 2022, 1751-7311

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30104 Pharmacology and pharmacy

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.600

Kód RIV

RIV/00216224:14160/22:00129447

Organizační jednotka

Farmaceutická fakulta

DOI

http://dx.doi.org/10.1016/j.animal.2021.100430

UT WoS

000826439700007

Klíčová slova anglicky

Fish; Maturation; Sperm motility; Sperm production; Sustained drug release

Štítky

rivok, ÚFT

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 2. 2023 13:23, JUDr. Sabina Krejčiříková

Anotace

V originále

Artificial reproduction of northern pike Esox lucius is impeded by the likelihood of obtaining only a small volume of sperm of inconsistent quality. A controlled-release hormone delivery system has the potential to enhance sperm production while avoiding multiple injections The objective of this study was to investigate the effects of mammalian gonadotropin-releasing hormone agonist (mGnRHa) incorporated into poly(lactic-co-glycolic acid) (PLGA) microparticles on milt production, spermatozoon characteristics, and secretion of 17β-estradiol (E2), 11-keto testosterone (11-KT), and testosterone in northern pike. Fish were divided into four groups and injected with 2 mg/kg BW carp pituitary extract (CPE), 20 µg/kg BW mGnRHa in PLGA microparticles, or 20 µg/kg BW mGnRHa plus 20 mg/kg BW metoclopramide (MET) in PLGA microparticles (PLGA + MET), along with a control group injected with 1 ml/kg 0.9% NaCl. At 48 h postinjection, the volume of milt produced was significantly greater in groups treated with CPE and PLGA + MET than in other groups. At 96 h postinjection, all hormone-treated groups exhibited significantly higher spermatozoon average velocity than recorded in the control group. Spermatozoon motility was significantly increased (P < 0.05) in the CPE and PLGA groups compared to baseline values. All treated groups showed significantly lower levels of 11-KT after the hormone injection compared to baseline values and to controls. Plasma testosterone levels increased in all hormone-treated groups. The use of PLGA microparticles, with or without metoclopramide, is suitable for use as a carrier of hormone treatments to regulate spermiation in mature northern pike.

Návaznosti

QK1810221, projekt VaV

Název: Využití mikročástic jako nosičů hormonálně aktivních látek v řízené reprodukci ryb.

Investor: Ministerstvo zemědělství ČR, Využití mikročástic jako nosičů hormonálně aktivních látek v řízené reprodukci ryb.

Citovat

KNOWLES, J., S. BORYSHPOLETS, V. KHOLODNYY, D. RAHI, Jakub VYSLOUŽIL, Jan MUSELÍK, V. STEJSKAL, J. KOURIL a P. PODHOREC. Effects of gonadotropin-releasing hormone agonist administered in microparticles on sperm quality and quantity, and plasma sex steroid levels in northern pike. ANIMAL. Amsterdam: Elsevier, 2022, roč. 16, č. 1, s. 1-8. ISSN 1751-7311. Dostupné z: https://dx.doi.org/10.1016/j.animal.2021.100430.

@article{2261737,
   author = {Knowles, J. and Boryshpolets, S. and Kholodnyy, V. and Rahi, D. and Vysloužil, Jakub and Muselík, Jan and Stejskal, V. and Kouril, J. and Podhorec, P.},
   article_location = {Amsterdam},
   article_number = {1},
   doi = {http://dx.doi.org/10.1016/j.animal.2021.100430},
   keywords = {Fish; Maturation; Sperm motility; Sperm production; Sustained drug release},
   language = {eng},
   issn = {1751-7311},
   journal = {ANIMAL},
   title = {Effects of gonadotropin-releasing hormone agonist administered in microparticles on sperm quality and quantity, and plasma sex steroid levels in northern pike},
   url = {https://www.sciencedirect.com/science/article/pii/S1751731121002767?via%3Dihub},
   volume = {16},
   year = {2022}
}

TY  - JOUR
ID  - 2261737
AU  - Knowles, J. - Boryshpolets, S. - Kholodnyy, V. - Rahi, D. - Vysloužil, Jakub - Muselík, Jan - Stejskal, V. - Kouril, J. - Podhorec, P.
PY  - 2022
TI  - Effects of gonadotropin-releasing hormone agonist administered in microparticles on sperm quality and quantity, and plasma sex steroid levels in northern pike
JF  - ANIMAL
VL  - 16
IS  - 1
SP  - 1-8
EP  - 1-8
PB  - Elsevier
SN  - 17517311
KW  - Fish
KW  - Maturation
KW  - Sperm motility
KW  - Sperm production
KW  - Sustained drug release
UR  - https://www.sciencedirect.com/science/article/pii/S1751731121002767?via%3Dihub
N2  - Artificial reproduction of northern pike Esox lucius is impeded by the likelihood of obtaining only a small volume of sperm of inconsistent quality. A controlled-release hormone delivery system has the potential to enhance sperm production while avoiding multiple injections The objective of this study was to investigate the effects of mammalian gonadotropin-releasing hormone agonist (mGnRHa) incorporated into poly(lactic-co-glycolic acid) (PLGA) microparticles on milt production, spermatozoon characteristics, and secretion of 17β-estradiol (E2), 11-keto testosterone (11-KT), and testosterone in northern pike. Fish were divided into four groups and injected with 2 mg/kg BW carp pituitary extract (CPE), 20 µg/kg BW mGnRHa in PLGA microparticles, or 20 µg/kg BW mGnRHa plus 20 mg/kg BW metoclopramide (MET) in PLGA microparticles (PLGA + MET), along with a control group injected with 1 ml/kg 0.9% NaCl. At 48 h postinjection, the volume of milt produced was significantly greater in groups treated with CPE and PLGA + MET than in other groups. At 96 h postinjection, all hormone-treated groups exhibited significantly higher spermatozoon average velocity than recorded in the control group. Spermatozoon motility was significantly increased (P < 0.05) in the CPE and PLGA groups compared to baseline values. All treated groups showed significantly lower levels of 11-KT after the hormone injection compared to baseline values and to controls. Plasma testosterone levels increased in all hormone-treated groups. The use of PLGA microparticles, with or without metoclopramide, is suitable for use as a carrier of hormone treatments to regulate spermiation in mature northern pike.
ER  -

KNOWLES, J., S. BORYSHPOLETS, V. KHOLODNYY, D. RAHI, Jakub VYSLOUŽIL, Jan MUSELÍK, V. STEJSKAL, J. KOURIL a P. PODHOREC. Effects of gonadotropin-releasing hormone agonist administered in microparticles on sperm quality and quantity, and plasma sex steroid levels in northern pike. \textit{ANIMAL}. Amsterdam: Elsevier, 2022, roč.~16, č.~1, s.~1-8. ISSN~1751-7311. Dostupné z: https://dx.doi.org/10.1016/j.animal.2021.100430.

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