Další formáty:
BibTeX
LaTeX
RIS
@article{2262437,
author = {Janata, Miroslav and Cadova, Eva and Angelisova, Pavla and Charnavets, Tatsiana and Horejsi, Vaclav and Raus, Vladimir},
article_location = {WEINHEIM},
article_number = {10},
doi = {http://dx.doi.org/10.1002/mabi.202200284},
keywords = {amphiphilic copolymers; isolation; membrane proteins; screening; solubilization},
language = {eng},
issn = {1616-5187},
journal = {Macromolecular Bioscience},
title = {Tailoring Butyl Methacrylate/Methacrylic Acid Copolymers for the Solubilization of Membrane Proteins: The Influence of Composition and Molecular Weight},
url = {https://onlinelibrary.wiley.com/doi/10.1002/mabi.202200284},
volume = {22},
year = {2022}
}
TY - JOUR
ID - 2262437
AU - Janata, Miroslav - Cadova, Eva - Angelisova, Pavla - Charnavets, Tatsiana - Horejsi, Vaclav - Raus, Vladimir
PY - 2022
TI - Tailoring Butyl Methacrylate/Methacrylic Acid Copolymers for the Solubilization of Membrane Proteins: The Influence of Composition and Molecular Weight
JF - Macromolecular Bioscience
VL - 22
IS - 10
SP - 2200284
EP - 2200284
PB - Wiley-VCH
SN - 16165187
KW - amphiphilic copolymers
KW - isolation
KW - membrane proteins
KW - screening
KW - solubilization
UR - https://onlinelibrary.wiley.com/doi/10.1002/mabi.202200284
N2 - Low-molecular weight (MW) amphiphilic copolymers have been recently introduced as a powerful tool for the detergent-free isolation of cell membrane proteins. Herein, a screening approach is used to identify a new copolymer type for this application. Via a two-step ATRP/acidolysis procedure, a 3 x 3 matrix of well-defined poly[(butyl methacrylate)-co-(methacrylic acid)] copolymers (denoted BMAA) differing in their MW and ratio of hydrophobic (BMA) and hydrophilic (MAA) units is prepared. Subsequently, using the biologically relevant model (T-cell line Jurkat), two compositions of BMAA copolymers are identified that solubilize cell membranes to an extent comparable to the industry standard, styrene-maleic acid copolymer (SMA), while avoiding the potentially problematic phenyl groups. Surprisingly, while only the lowest-MW variant of the BMA/MAA 2:1 composition is effective, all the copolymers of the BMA/MAA 1:1 composition are found to solubilize the model membranes, including the high-MW variant (MW of 14 000). Importantly, the density gradient ultracentrifugation/sodium dodecyl sulfate-polyacrylamide gel electrophoresis/Western blotting experiments reveal that the BMA/MAA 1:1 copolymers disintegrate the Jurkat membranes differently than SMA, as demonstrated by the different distribution patterns of two tested membrane protein markers. This makes the BMAA copolymers a useful tool for studies on membrane microdomains differing in their composition and resistance to membrane-disintegrating polymers.
ER -
JANATA, Miroslav, Eva CADOVA, Pavla ANGELISOVA, Tatsiana CHARNAVETS, Vaclav HOREJSI a Vladimir RAUS. Tailoring Butyl Methacrylate/Methacrylic Acid Copolymers for the Solubilization of Membrane Proteins: The Influence of Composition and Molecular Weight. \textit{Macromolecular Bioscience}. WEINHEIM: Wiley-VCH, 2022, roč.~22, č.~10, s.~2200284-2200290. ISSN~1616-5187. Dostupné z: https://dx.doi.org/10.1002/mabi.202200284.
|
