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@article{2262566,
author = {Rada, Petr and Hrdy, Ivan and Zdrha, Alois and Narayanasamy, Ravi Kumar and Smutna, Tamara and Horackova, Jana and Harant, Karel and Benes, Vladimir and Ong, SeowandChin and Tsai, ChihandYu and Luo, HongandWei and Chiu, ChengandHsun and Tang, Petrus and Tachezy, Jan},
article_location = {Switzerland},
article_number = {MAY},
doi = {http://dx.doi.org/10.3389/fmicb.2022.893692},
keywords = {Trichomonasvirus; TVV; exosome; extracellular vesicle; proteomics; tsRNA},
language = {eng},
issn = {1664-302X},
journal = {Frontiers in Microbiology},
title = {Double-Stranded RNA Viruses Are Released From Trichomonas vaginalis Inside Small Extracellular Vesicles and Modulate the Exosomal Cargo},
url = {https://www.frontiersin.org/articles/10.3389/fmicb.2022.893692/full},
volume = {13},
year = {2022}
}
TY - JOUR
ID - 2262566
AU - Rada, Petr - Hrdy, Ivan - Zdrha, Alois - Narayanasamy, Ravi Kumar - Smutna, Tamara - Horackova, Jana - Harant, Karel - Benes, Vladimir - Ong, Seow-Chin - Tsai, Chih-Yu - Luo, Hong-Wei - Chiu, Cheng-Hsun - Tang, Petrus - Tachezy, Jan
PY - 2022
TI - Double-Stranded RNA Viruses Are Released From Trichomonas vaginalis Inside Small Extracellular Vesicles and Modulate the Exosomal Cargo
JF - Frontiers in Microbiology
VL - 13
IS - MAY
SP - 893692
EP - 893692
PB - Frontiers Research Foundation
SN - 1664302X
KW - Trichomonasvirus
KW - TVV
KW - exosome
KW - extracellular vesicle
KW - proteomics
KW - tsRNA
UR - https://www.frontiersin.org/articles/10.3389/fmicb.2022.893692/full
N2 - Trichomonas vaginalis is a parasitic protist that infects the human urogenital tract. During the infection, trichomonads adhere to the host mucosa, acquire nutrients from the vaginal/prostate environment, and release small extracellular vesicles (sEVs) that contribute to the trichomonad adherence and modulate the host-parasite communication. Approximately 40-70% of T. vaginalis strains harbor a double-stranded RNA virus called Trichomonasvirus (TVV). Naked TVV particles have the potential to stimulate a proinflammatory response in human cells, however, the mode of TVV release from trichomonads to the environment is not clear. In this report, we showed for the first time that TVV particles are released from T. vaginalis cells within sEVs. The sEVs loaded with TVV stimulated a higher proinflammatory response of human HaCaT cells in comparison to sEVs from TVV negative parasites. Moreover, a comparison of T. vaginalis isogenic TVV plus and TVV minus clones revealed a significant impact of TVV infection on the sEV proteome and RNA cargo. Small EVs from TVV positive trichomonads contained 12 enriched and 8 unique proteins including membrane-associated BspA adhesine, and about a 2.5-fold increase in the content of small regulatory tsRNA. As T. vaginalis isolates are frequently infected with TVV, the release of TVV via sEVs to the environment represents an important factor with the potential to enhance inflammation-related pathogenesis during trichomoniasis.
ER -
RADA, Petr, Ivan HRDY, Alois ZDRHA, Ravi Kumar NARAYANASAMY, Tamara SMUTNA, Jana HORACKOVA, Karel HARANT, Vladimir BENES, Seow-Chin ONG, Chih-Yu TSAI, Hong-Wei LUO, Cheng-Hsun CHIU, Petrus TANG and Jan TACHEZY. Double-Stranded RNA Viruses Are Released From Trichomonas vaginalis Inside Small Extracellular Vesicles and Modulate the Exosomal Cargo. \textit{Frontiers in Microbiology}. Switzerland: Frontiers Research Foundation, 2022, vol.~13, MAY, p.~893692-893711. ISSN~1664-302X. Available from: https://dx.doi.org/10.3389/fmicb.2022.893692.
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