A cytosolic ferredoxin-independent hydrogenase possibly
mediates hydrogen uptake in Trichomonas vaginalis

J 2022

A cytosolic ferredoxin-independent hydrogenase possibly mediates hydrogen uptake in Trichomonas vaginalis

SMUTNA, Tamara, Alena DOHNALKOVA, Robert SUTAK, Ravi Kumar NARAYANASAMY, Jan TACHEZY et. al.

Základní údaje

Originální název

A cytosolic ferredoxin-independent hydrogenase possibly mediates hydrogen uptake in Trichomonas vaginalis

Autoři

SMUTNA, Tamara, Alena DOHNALKOVA, Robert SUTAK, Ravi Kumar NARAYANASAMY, Jan TACHEZY a Ivan HRDY

Vydání

Current Biology, Elsevier Science, 2022, 0960-9822

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 9.200

Kód RIV

RIV/00216224:14740/22:00128781

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.cub.2021.10.050

UT WoS

000743814700011

Klíčová slova anglicky

Cytosol; Eukaryota; Ferredoxins; Humans; Hydrogen; Hydrogenase; Trichomonas vaginalis

Štítky

ne MU, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 4. 2023 10:47, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Trichomonads, represented by the highly prevalent sexually transmitted human parasite Trichomonas vaginalis, are anaerobic eukaryotes with hydrogenosomes in the place of the standard mitochondria. Hydrogenosomes form indispensable FeS-clusters, synthesize ATP, and release molecular hydrogen as a waste product. Hydrogen formation is catalyzed by [FeFe] hydrogenase, the hallmark enzyme of all hydrogenosomes found in various eukaryotic anaerobes. Eukaryotic hydrogenases were originally thought to be exclusively localized within organelles, but today few eukaryotic anaerobes are known that possess hydrogenase in their cytosol. We identified a thus-far unknown hydrogenase in T. vaginalis cytosol that cannot use ferredoxin as a redox partner but can use cytochrome b5 as an electron acceptor. Trichomonads overexpressing the cytosolic hydrogenase, while maintaining the carbon flux through hydrogenosomes, show decreased excretion of hydrogen and increased excretion of methylated alcohols, suggesting that the cytosolic hydrogenase uses the hydrogen gas as a source of reducing power for the reactions occurring in the cytoplasm and thus accounts for the overall redox balance. This is the first evidence of hydrogen uptake in a eukaryote, although further work is needed to confirm it. Assembly of the catalytic center of [FeFe] hydrogenases (H-cluster) requires the activity of three dedicated maturases, and these proteins in T. vaginalis are exclusively localized in hydrogenosomes, where they participate in the maturation of organellar hydrogenases. Despite the different subcellular localization of cytosolic hydrogenase and maturases, the H-cluster is present in the cytosolic enzyme, suggesting the existence of an alternative mechanism of H-cluster assembly.

Návaznosti

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

SMUTNA, Tamara, Alena DOHNALKOVA, Robert SUTAK, Ravi Kumar NARAYANASAMY, Jan TACHEZY a Ivan HRDY. A cytosolic ferredoxin-independent hydrogenase possibly mediates hydrogen uptake in Trichomonas vaginalis. Current Biology. Elsevier Science, 2022, roč. 32, č. 1, s. 124-135, 18 s. ISSN 0960-9822. Dostupné z: https://dx.doi.org/10.1016/j.cub.2021.10.050.

@article{2262572,
   author = {Smutna, Tamara and Dohnalkova, Alena and Sutak, Robert and Narayanasamy, Ravi Kumar and Tachezy, Jan and Hrdy, Ivan},
   article_number = {1},
   doi = {http://dx.doi.org/10.1016/j.cub.2021.10.050},
   keywords = {Cytosol; Eukaryota; Ferredoxins; Humans; Hydrogen; Hydrogenase; Trichomonas vaginalis},
   language = {eng},
   issn = {0960-9822},
   journal = {Current Biology},
   title = {A cytosolic ferredoxin-independent hydrogenase possibly mediates hydrogen uptake in Trichomonas vaginalis},
   url = {https://www.sciencedirect.com/science/article/pii/S0960982221014949?via%3Dihub},
   volume = {32},
   year = {2022}
}

TY  - JOUR
ID  - 2262572
AU  - Smutna, Tamara - Dohnalkova, Alena - Sutak, Robert - Narayanasamy, Ravi Kumar - Tachezy, Jan - Hrdy, Ivan
PY  - 2022
TI  - A cytosolic ferredoxin-independent hydrogenase possibly mediates hydrogen uptake in Trichomonas vaginalis
JF  - Current Biology
VL  - 32
IS  - 1
SP  - 124-135
EP  - 124-135
PB  - Elsevier Science
SN  - 09609822
KW  - Cytosol
KW  - Eukaryota
KW  - Ferredoxins
KW  - Humans
KW  - Hydrogen
KW  - Hydrogenase
KW  - Trichomonas vaginalis
UR  - https://www.sciencedirect.com/science/article/pii/S0960982221014949?via%3Dihub
N2  - Trichomonads, represented by the highly prevalent sexually transmitted human parasite Trichomonas vaginalis, are anaerobic eukaryotes with hydrogenosomes in the place of the standard mitochondria. Hydrogenosomes form indispensable FeS-clusters, synthesize ATP, and release molecular hydrogen as a waste product. Hydrogen formation is catalyzed by [FeFe] hydrogenase, the hallmark enzyme of all hydrogenosomes found in various eukaryotic anaerobes. Eukaryotic hydrogenases were originally thought to be exclusively localized within organelles, but today few eukaryotic anaerobes are known that possess hydrogenase in their cytosol. We identified a thus-far unknown hydrogenase in T. vaginalis cytosol that cannot use ferredoxin as a redox partner but can use cytochrome b5 as an electron acceptor. Trichomonads overexpressing the cytosolic hydrogenase, while maintaining the carbon flux through hydrogenosomes, show decreased excretion of hydrogen and increased excretion of methylated alcohols, suggesting that the cytosolic hydrogenase uses the hydrogen gas as a source of reducing power for the reactions occurring in the cytoplasm and thus accounts for the overall redox balance. This is the first evidence of hydrogen uptake in a eukaryote, although further work is needed to confirm it. Assembly of the catalytic center of [FeFe] hydrogenases (H-cluster) requires the activity of three dedicated maturases, and these proteins in T. vaginalis are exclusively localized in hydrogenosomes, where they participate in the maturation of organellar hydrogenases. Despite the different subcellular localization of cytosolic hydrogenase and maturases, the H-cluster is present in the cytosolic enzyme, suggesting the existence of an alternative mechanism of H-cluster assembly.
ER  -

SMUTNA, Tamara, Alena DOHNALKOVA, Robert SUTAK, Ravi Kumar NARAYANASAMY, Jan TACHEZY a Ivan HRDY. A cytosolic ferredoxin-independent hydrogenase possibly mediates hydrogen uptake in Trichomonas vaginalis. \textit{Current Biology}. Elsevier Science, 2022, roč.~32, č.~1, s.~124-135, 18 s. ISSN~0960-9822. Dostupné z: https://dx.doi.org/10.1016/j.cub.2021.10.050.

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