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@article{2263177,
author = {Sladeček, Stanislava and Radaszkiewicz, Katarzyna Anna and Bőhmová, Martina and Gybeľ, Tomáš and Radaszkiewicz, Tomasz Witold and Pacherník, Jiří},
article_location = {San Francisco},
article_number = {10},
doi = {http://dx.doi.org/10.1371/journal.pone.0275860},
keywords = {Cell differentiation; Cardiomyocytes; Cell staining; Heart; Mesoderm; Small interfering RNA; Phosphorylation; MAPK signaling cascades},
language = {eng},
issn = {1932-6203},
journal = {Plos one},
title = {Dual specificity phosphatase 7 drives the formation of cardiac mesoderm in mouse embryonic stem cells},
url = {https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0275860},
volume = {17},
year = {2022}
}
TY - JOUR
ID - 2263177
AU - Sladeček, Stanislava - Radaszkiewicz, Katarzyna Anna - Bőhmová, Martina - Gybeľ, Tomáš - Radaszkiewicz, Tomasz Witold - Pacherník, Jiří
PY - 2022
TI - Dual specificity phosphatase 7 drives the formation of cardiac mesoderm in mouse embryonic stem cells
JF - Plos one
VL - 17
IS - 10
SP - 1-22
EP - 1-22
PB - Public Library of Science
SN - 19326203
KW - Cell differentiation
KW - Cardiomyocytes
KW - Cell staining
KW - Heart
KW - Mesoderm
KW - Small interfering RNA
KW - Phosphorylation
KW - MAPK signaling cascades
UR - https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0275860
N2 - Dual specificity phosphatase 7 (DUSP7) is a protein belonging to a broad group of phosphatases that can dephosphorylate phosphoserine/phosphothreonine as well as phosphotyrosine residues within the same substrate. DUSP7 has been linked to the negative regulation of mitogen activated protein kinases (MAPK), and in particular to the regulation of extracellular signal-regulated kinases 1 and 2 (ERK1/2). MAPKs play an important role in embryonic development, where their duration, magnitude, and spatiotemporal activity must be strictly controlled by other proteins, among others by DUSPs. In this study, we focused on the effect of DUSP7 depletion on the in vitro differentiation of mouse embryonic stem (ES) cells. We showed that even though DUSP7 knock-out ES cells do retain some of their basic characteristics, when it comes to differentiation, they preferentially differentiate towards neural cells, while the formation of early cardiac mesoderm is repressed. Therefore, our data indicate that DUSP7 is necessary for the correct formation of neuroectoderm and cardiac mesoderm during the in vitro differentiation of ES cells.
ER -
SLADEČEK, Stanislava, Katarzyna Anna RADASZKIEWICZ, Martina B$\backslash$H OHMOVÁ, Tomáš GYBEĽ, Tomasz Witold RADASZKIEWICZ a Jiří PACHERNÍK. Dual specificity phosphatase 7 drives the formation of cardiac mesoderm in mouse embryonic stem cells. \textit{Plos one}. San Francisco: Public Library of Science, 2022, roč.~17, č.~10, s.~1-22. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0275860.
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