a 2022

Development of lectin arrays for mapping the glycosylation changes in proteins and cells

KOMÁREK, Jan, Marek KORSÁK, Lukáš FALTINEK a Michaela WIMMEROVÁ

Základní údaje

Originální název

Development of lectin arrays for mapping the glycosylation changes in proteins and cells

Autoři

KOMÁREK, Jan (203 Česká republika, domácí), Marek KORSÁK (703 Slovensko, domácí), Lukáš FALTINEK (203 Česká republika, domácí) a Michaela WIMMEROVÁ (203 Česká republika, garant, domácí)

Vydání

XXIst Meeting of Biochemists and Molecular Biologists, 2022

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/22:00129422

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-280-0136-0

Klíčová slova česky

lektiny, glykosylace, lektinové čipy

Klíčová slova anglicky

lectins; glycosylation; lectin arrays
Změněno: 6. 3. 2023 14:13, Mgr. Jan Komárek, Ph.D.

Anotace

V originále

Saccharides are essential for life, and in addition to their traditional roles as structural and energy storage components, they play crucial roles in many recognition events, signaling, and communication processes. The glycosylation of cells reflects the biological species, tissue, and physiological state of the organism. Importantly, changes in glycosylation patterns were observed in various tumors, and the aberrant glycosylation is often associated with malignancy potential, tumor immune surveillance, and patients´ prognosis. Lectin arrays are widely used tools for analyzing glycosylation in proteins and cells. They utilize a panel of lectins (saccharide-binding proteins with distinct sugar specificities) immobilized onto a solid surface. Lectin arrays allow for rapid and high-throughput glycomic analysis with minimal sample consumption. They can be used for glycan profiling of various samples, including purified glycoproteins, cell/tissue lysates, membrane vesicles, complex biological samples, or entire cells. This talk will present the development of customized lectin arrays in two different formats. The classical array in the microscope slide format was prepared by non-contact piezo dispensing of lectins onto a functionalized glass slide and their immobilization using various surface chemistries (immobilization on epoxy groups, NHS/EDC coupling). In the slide format, binding of glycosylated samples was detected by fluorescence measurement. To allow label-free detection of attached glyco-samples, lectin microarray prepared on bio-layer interferometry (BLI) sensor tips is currently being optimized. The fabricated lectin arrays will be used to map the glycosylation changes at different stages of cell differentiation and under different physiopathological conditions.

Návaznosti

GA21-29622S, projekt VaV
Název: Biologická úloha lektinů v symbióze a patogenezi
Investor: Grantová agentura ČR, Biologická úloha lektinů v symbióze a patogenezi