Pea root responses under naproxen stress: changes in the
formation of structural barriers in the primary root in context
with changes of auxin and abscisic acid levels

J 2023

Pea root responses under naproxen stress: changes in the formation of structural barriers in the primary root in context with changes of auxin and abscisic acid levels

SVOBODNÍKOVÁ, Lucie, Marie KUMMEROVÁ, Štěpán ZEZULKA, Michal MARTINKA, Marek KLEMŠ et. al.

Základní údaje

Originální název

Pea root responses under naproxen stress: changes in the formation of structural barriers in the primary root in context with changes of auxin and abscisic acid levels

Autoři

SVOBODNÍKOVÁ, Lucie (203 Česká republika, domácí), Marie KUMMEROVÁ (203 Česká republika, domácí), Štěpán ZEZULKA (203 Česká republika, garant, domácí), Michal MARTINKA, Marek KLEMŠ a Josef ČÁSLAVSKÝ

Vydání

Ecotoxicology, Springer, 2023, 0963-9292

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.700 v roce 2022

Kód RIV

RIV/00216224:14310/23:00130419

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1007/s10646-022-02613-8

UT WoS

001062603700001

Klíčová slova anglicky

NSAID; Root growth dynamics; Casparian strips; Suberin lamellae; Phytohormones

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 11. 2023 12:16, Mgr. Marie Šípková, DiS.

Anotace

V originále

Pharmaceuticals belong to pseudo-persistent pollutants because of constant entry into the environment and hazardous potential for non-target organisms, including plants, in which they can influence biochemical and physiological processes. Detailed analysis of results obtained by microscopic observations using fluorescent dyes (berberine hemisulphate, Fluorol Yellow 088), detection of phytohormone levels (radioimmunoassay, enzyme-linked immune sorbent assay) and thermogravimetric analysis of lignin content proved that the drug naproxen (NPX) can stimulate the formation of root structural barriers. In the primary root of plants treated with 0.5, 1, and 10 mg/L NPX, earlier Casparian strip formation and development of the whole endodermis circle closer to its apex were found after five days of cultivation (by 9–20% as compared to control) and after ten days from 0.1 mg/L NPX (by 8–63%). Suberin lamellae (SL) were deposited in endodermal cells significantly closer to the apex under 10 mg/L NPX by up to 75%. Structural barrier formation under NPX treatment can be influenced indirectly by auxin-supported cell division and differentiation caused by its eight-times higher level under 10 mg/L NPX and directly by stimulated SL deposition induced by abscisic acid (higher from 0.5 mg/L NPX), as proved by the higher proportion of cells with SL in the primary root base (by 8–44%). The earlier modification of endodermis in plant roots can help to limit the drug transfer and maintain the homeostasis of the plant.

Návaznosti

EF19_073/0016943, projekt VaV

Název: Interní grantová agentura Masarykovy univerzity

Citovat

SVOBODNÍKOVÁ, Lucie, Marie KUMMEROVÁ, Štěpán ZEZULKA, Michal MARTINKA, Marek KLEMŠ a Josef ČÁSLAVSKÝ. Pea root responses under naproxen stress: changes in the formation of structural barriers in the primary root in context with changes of auxin and abscisic acid levels. Ecotoxicology. Springer, 2023, roč. 32, č. 1, s. 1-11. ISSN 0963-9292. Dostupné z: https://dx.doi.org/10.1007/s10646-022-02613-8.

@article{2265597,
   author = {Svobodníková, Lucie and Kummerová, Marie and Zezulka, Štěpán and Martinka, Michal and Klemš, Marek and Čáslavský, Josef},
   article_number = {1},
   doi = {http://dx.doi.org/10.1007/s10646-022-02613-8},
   keywords = {NSAID; Root growth dynamics; Casparian strips; Suberin lamellae; Phytohormones},
   language = {eng},
   issn = {0963-9292},
   journal = {Ecotoxicology},
   title = {Pea root responses under naproxen stress: changes in the formation of structural barriers in the primary root in context with changes of auxin and abscisic acid levels},
   url = {https://doi.org/10.1007/s10646-022-02613-8},
   volume = {32},
   year = {2023}
}

TY  - JOUR
ID  - 2265597
AU  - Svobodníková, Lucie - Kummerová, Marie - Zezulka, Štěpán - Martinka, Michal - Klemš, Marek - Čáslavský, Josef
PY  - 2023
TI  - Pea root responses under naproxen stress: changes in the formation of structural barriers in the primary root in context with changes of auxin and abscisic acid levels
JF  - Ecotoxicology
VL  - 32
IS  - 1
SP  - 1-11
EP  - 1-11
PB  - Springer
SN  - 09639292
KW  - NSAID
KW  - Root growth dynamics
KW  - Casparian strips
KW  - Suberin lamellae
KW  - Phytohormones
UR  - https://doi.org/10.1007/s10646-022-02613-8
N2  - Pharmaceuticals belong to pseudo-persistent pollutants because of constant entry into the environment and hazardous potential for non-target organisms, including plants, in which they can influence biochemical and physiological processes. Detailed analysis of results obtained by microscopic observations using fluorescent dyes (berberine hemisulphate, Fluorol Yellow 088), detection of phytohormone levels (radioimmunoassay, enzyme-linked immune sorbent assay) and thermogravimetric analysis of lignin content proved that the drug naproxen (NPX) can stimulate the formation of root structural barriers. In the primary root of plants treated with 0.5, 1, and 10 mg/L NPX, earlier Casparian strip formation and development of the whole endodermis circle closer to its apex were found after five days of cultivation (by 9–20% as compared to control) and after ten days from 0.1 mg/L NPX (by 8–63%). Suberin lamellae (SL) were deposited in endodermal cells significantly closer to the apex under 10 mg/L NPX by up to 75%. Structural barrier formation under NPX treatment can be influenced indirectly by auxin-supported cell division and differentiation caused by its eight-times higher level under 10 mg/L NPX and directly by stimulated SL deposition induced by abscisic acid (higher from 0.5 mg/L NPX), as proved by the higher proportion of cells with SL in the primary root base (by 8–44%). The earlier modification of endodermis in plant roots can help to limit the drug transfer and maintain the homeostasis of the plant.
ER  -

SVOBODNÍKOVÁ, Lucie, Marie KUMMEROVÁ, Štěpán ZEZULKA, Michal MARTINKA, Marek KLEMŠ a Josef ČÁSLAVSKÝ. Pea root responses under naproxen stress: changes in the formation of structural barriers in the primary root in context with changes of auxin and abscisic acid levels. \textit{Ecotoxicology}. Springer, 2023, roč.~32, č.~1, s.~1-11. ISSN~0963-9292. Dostupné z: https://dx.doi.org/10.1007/s10646-022-02613-8.

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