The role of bivalent ions in the regulation of D-loop extension
mediated by DMC1 during meiotic recombination

J 2022

The role of bivalent ions in the regulation of D-loop extension mediated by DMC1 during meiotic recombination

ALTMANNOVÁ, Veronika, Mário ŠPÍREK, Lucija ORLIC, Atis JEKABSONS, Tereza CLARENCE et. al.

Základní údaje

Originální název

The role of bivalent ions in the regulation of D-loop extension mediated by DMC1 during meiotic recombination

Autoři

ALTMANNOVÁ, Veronika (203 Česká republika, domácí), Mário ŠPÍREK (703 Slovensko, domácí), Lucija ORLIC, Atis JEKABSONS (428 Lotyšsko, domácí), Tereza CLARENCE, Adrian HENGGELER, Jarmila MLCOUSKOVA, Raphael A G CHALEIL, Joao MATOS a Lumír KREJČÍ (203 Česká republika, garant, domácí)

Vydání

iSCIENCE, CAMBRIDGE, CELL PRESS, 2022, 2589-0042

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.800

Kód RIV

RIV/00216224:14110/22:00128839

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.isci.2022.105439

UT WoS

000923030100007

Klíčová slova anglicky

DMC1; meiotic recombination; D-loop extension; bivalent ions

Štítky

14110513, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 3. 2023 08:59, Mgr. Tereza Miškechová

Anotace

V originále

During meiosis, programmed DNA double-strand breaks (DSBs) are repaired by homologous recombination. DMC1, a conserved recombinase, plays a central role in this process. DMC1 promotes DNA strand exchange between homologous chromosomes, thus creating the physical linkage between them. Its function is regulated not only by several accessory proteins but also by bivalent ions. Here, we show that whereas calcium ions in the presence of ATP cause a conformational change within DMC1, stimulating its DNA binding and D-loop formation, they inhibit the extension of the invading strand within the D-loop. Based on structural studies, we have generated mutants of two highly conserved amino acids - E162 and D317 - in human DMC1, which are deficient in calcium regulation. In vivo studies of their yeast homologues further showed that they exhibit severe defects in meiosis, thus emphasizing the importance of calcium ions in the regulation of DMC1 function and meiotic recombination.

Návaznosti

EF16_025/0007381, projekt VaV

Název: Preklinická progrese nových organických sloučenin s cílenou biologickou aktivitou

GX21-22593X, projekt VaV

Název: Identifikace a charakterizace proteinů zahrnutých v metabolismu G-kvadruplexů a R-smyček a jejich vztah k replikaci DNA

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LM2018140, projekt VaV

Název: e-Infrastruktura CZ (Akronym: e-INFRA CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, e-Infrastruktura CZ

MUNI/G/1594/2019, interní kód MU

Název: RecQ4 – a protein hub required for proper replication and recombination and its implications in Rothmund-Thomson Syndrome

Investor: Masarykova univerzita, RecQ4 – a protein hub required for proper replication and recombination and its implications in Rothmund-Thomson Syndrome, INTERDISCIPLINARY - Mezioborové výzkumné projekty

206292/E/17/Z, interní kód MU

Název: Mechanics and execution of homologous recombination - biophysics to the organism

Investor: Wellcome Trust, Mechanics and execution of homologous recombination - biophysics to the organism

Citovat

ALTMANNOVÁ, Veronika, Mário ŠPÍREK, Lucija ORLIC, Atis JEKABSONS, Tereza CLARENCE, Adrian HENGGELER, Jarmila MLCOUSKOVA, Raphael A G CHALEIL, Joao MATOS a Lumír KREJČÍ. The role of bivalent ions in the regulation of D-loop extension mediated by DMC1 during meiotic recombination. iSCIENCE. CAMBRIDGE: CELL PRESS, 2022, roč. 25, č. 11, s. 1-26. ISSN 2589-0042. Dostupné z: https://dx.doi.org/10.1016/j.isci.2022.105439.

@article{2266078,
   author = {Altmannová, Veronika and Špírek, Mário and Orlic, Lucija and Jekabsons, Atis and Clarence, Tereza and Henggeler, Adrian and Mlcouskova, Jarmila and Chaleil, Raphael A G and Matos, Joao and Krejčí, Lumír},
   article_location = {CAMBRIDGE},
   article_number = {11},
   doi = {http://dx.doi.org/10.1016/j.isci.2022.105439},
   keywords = {DMC1; meiotic recombination; D-loop extension; bivalent ions},
   language = {eng},
   issn = {2589-0042},
   journal = {iSCIENCE},
   title = {The role of bivalent ions in the regulation of D-loop extension mediated by DMC1 during meiotic recombination},
   url = {https://www.cell.com/iscience/fulltext/S2589-0042(22)01711-4?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS2589004222017114%3Fshowall%3Dtrue},
   volume = {25},
   year = {2022}
}

TY  - JOUR
ID  - 2266078
AU  - Altmannová, Veronika - Špírek, Mário - Orlic, Lucija - Jekabsons, Atis - Clarence, Tereza - Henggeler, Adrian - Mlcouskova, Jarmila - Chaleil, Raphael A G - Matos, Joao - Krejčí, Lumír
PY  - 2022
TI  - The role of bivalent ions in the regulation of D-loop extension mediated by DMC1 during meiotic recombination
JF  - iSCIENCE
VL  - 25
IS  - 11
SP  - 1-26
EP  - 1-26
PB  - CELL PRESS
SN  - 25890042
KW  - DMC1
KW  - meiotic recombination
KW  - D-loop extension
KW  - bivalent ions
UR  - https://www.cell.com/iscience/fulltext/S2589-0042(22)01711-4?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS2589004222017114%3Fshowall%3Dtrue
N2  - During meiosis, programmed DNA double-strand breaks (DSBs) are repaired by homologous recombination. DMC1, a conserved recombinase, plays a central role in this process. DMC1 promotes DNA strand exchange between homologous chromosomes, thus creating the physical linkage between them. Its function is regulated not only by several accessory proteins but also by bivalent ions. Here, we show that whereas calcium ions in the presence of ATP cause a conformational change within DMC1, stimulating its DNA binding and D-loop formation, they inhibit the extension of the invading strand within the D-loop. Based on structural studies, we have generated mutants of two highly conserved amino acids - E162 and D317 - in human DMC1, which are deficient in calcium regulation. In vivo studies of their yeast homologues further showed that they exhibit severe defects in meiosis, thus emphasizing the importance of calcium ions in the regulation of DMC1 function and meiotic recombination.
ER  -

ALTMANNOVÁ, Veronika, Mário ŠPÍREK, Lucija ORLIC, Atis JEKABSONS, Tereza CLARENCE, Adrian HENGGELER, Jarmila MLCOUSKOVA, Raphael A G CHALEIL, Joao MATOS a Lumír KREJČÍ. The role of bivalent ions in the regulation of D-loop extension mediated by DMC1 during meiotic recombination. \textit{iSCIENCE}. CAMBRIDGE: CELL PRESS, 2022, roč.~25, č.~11, s.~1-26. ISSN~2589-0042. Dostupné z: https://dx.doi.org/10.1016/j.isci.2022.105439.

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