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@article{2266078,
author = {Altmannová, Veronika and Špírek, Mário and Orlic, Lucija and Jekabsons, Atis and Clarence, Tereza and Henggeler, Adrian and Mlcouskova, Jarmila and Chaleil, Raphael A G and Matos, Joao and Krejčí, Lumír},
article_location = {CAMBRIDGE},
article_number = {11},
doi = {http://dx.doi.org/10.1016/j.isci.2022.105439},
keywords = {DMC1; meiotic recombination; D-loop extension; bivalent ions},
language = {eng},
issn = {2589-0042},
journal = {iSCIENCE},
title = {The role of bivalent ions in the regulation of D-loop extension mediated by DMC1 during meiotic recombination},
url = {https://www.cell.com/iscience/fulltext/S2589-0042(22)01711-4?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS2589004222017114%3Fshowall%3Dtrue},
volume = {25},
year = {2022}
}
TY - JOUR
ID - 2266078
AU - Altmannová, Veronika - Špírek, Mário - Orlic, Lucija - Jekabsons, Atis - Clarence, Tereza - Henggeler, Adrian - Mlcouskova, Jarmila - Chaleil, Raphael A G - Matos, Joao - Krejčí, Lumír
PY - 2022
TI - The role of bivalent ions in the regulation of D-loop extension mediated by DMC1 during meiotic recombination
JF - iSCIENCE
VL - 25
IS - 11
SP - 1-26
EP - 1-26
PB - CELL PRESS
SN - 25890042
KW - DMC1
KW - meiotic recombination
KW - D-loop extension
KW - bivalent ions
UR - https://www.cell.com/iscience/fulltext/S2589-0042(22)01711-4?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS2589004222017114%3Fshowall%3Dtrue
N2 - During meiosis, programmed DNA double-strand breaks (DSBs) are repaired by homologous recombination. DMC1, a conserved recombinase, plays a central role in this process. DMC1 promotes DNA strand exchange between homologous chromosomes, thus creating the physical linkage between them. Its function is regulated not only by several accessory proteins but also by bivalent ions. Here, we show that whereas calcium ions in the presence of ATP cause a conformational change within DMC1, stimulating its DNA binding and D-loop formation, they inhibit the extension of the invading strand within the D-loop. Based on structural studies, we have generated mutants of two highly conserved amino acids - E162 and D317 - in human DMC1, which are deficient in calcium regulation. In vivo studies of their yeast homologues further showed that they exhibit severe defects in meiosis, thus emphasizing the importance of calcium ions in the regulation of DMC1 function and meiotic recombination.
ER -
ALTMANNOVÁ, Veronika, Mário ŠPÍREK, Lucija ORLIC, Atis JEKABSONS, Tereza CLARENCE, Adrian HENGGELER, Jarmila MLCOUSKOVA, Raphael A G CHALEIL, Joao MATOS a Lumír KREJČÍ. The role of bivalent ions in the regulation of D-loop extension mediated by DMC1 during meiotic recombination. \textit{iSCIENCE}. CAMBRIDGE: CELL PRESS, 2022, roč.~25, č.~11, s.~1-26. ISSN~2589-0042. Dostupné z: https://dx.doi.org/10.1016/j.isci.2022.105439.
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