Vitality and growth rate of agar plate-cultivated Antarctic
microautotrophs: Analysis of PSII functioning by chlorophyll
fluorescence parameters

J 2022

Vitality and growth rate of agar plate-cultivated Antarctic microautotrophs: Analysis of PSII functioning by chlorophyll fluorescence parameters

MANOVÁ, Anna, Blanka HÝŽOVÁ, Diana Darriba CANORA, Ana Castrillo ANTOLIN, Kristýna DUFKOVÁ et. al.

Základní údaje

Originální název

Vitality and growth rate of agar plate-cultivated Antarctic microautotrophs: Analysis of PSII functioning by chlorophyll fluorescence parameters

Autoři

MANOVÁ, Anna (203 Česká republika, domácí), Blanka HÝŽOVÁ (203 Česká republika, domácí), Diana Darriba CANORA (724 Španělsko), Ana Castrillo ANTOLIN (724 Španělsko) a Kristýna DUFKOVÁ (203 Česká republika, garant, domácí)

Vydání

Czech polar reports, Brno, Masaryk University, 2022, 1805-0689

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.000

Kód RIV

RIV/00216224:14310/22:00128888

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.5817/CPR2022-2-20

UT WoS

000935360100010

Klíčová slova anglicky

lichen photobionts; algal biotechnology; Kautsky kinetics

Štítky

14110212, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 7. 2023 10:12, Mgr. Marie Šípková, DiS.

Anotace

V originále

In our study, we focused on the growth of three different microautotrophs isolated from Antarctic lichens (Placopsis contortuplicata, Solorina spongiosa) and cryptoendolithic algal vegetation. The isolates were purified and inoculated on agar plates, Bold ' s Basal Medium (BBM). The growth of the cultures and the markers of physiological (photo-synthetic) activity were monitored by chlorophyll fluorescence in 1 week intervals for 3 months after inoculation. For the assessment of photosynthetic activity, the meth-od of slow Kautsky kinetics supplemented with saturation pulses was applied. Four chlorophyll fluorescence parameters calculated: (1) maximum quantum yield of PSII (FV/FM), (2) effective quantum yield of photosynthetic processes in PSII (phi PSII), (3) non -photochemical quenching of chlorophyll fluorescence, and (4) background chlorophyll fluorescence ratio (F0/F0 '). Troughout the cultivation period, the maximum quantum yield of PSII (FV/FM) showed high values in all three autotrophs with only slight increase in the first part of the cultivation period, followed by slight decrease in the second part. The phi PSII values showed a rapid decline within the first 4 weeks of cultivation followed by more or less constant values in the isolates from P. contortuplicata and cryptoen-dolithic alga. Contrastingly, time course of phi PSII rather showed an increase followed by a decrease in S. spongiosa isolate. NPQ (related to the activation of protective mecha-nisms) increased in the second part of cultivation period, the rate of increase and maxi-mum values were species-specific. The species-specific differences in chlorophyll fluo-rescence parameters are discussed as well as their potential for evaluation of photo-synthetic performance of in vitro cultivated algal/cyanobacterial cultures on agar plates.

Návaznosti

EF16_013/0001708, projekt VaV

Název: ECOPOLARIS - Změny ve struktuře a funkci součástí terestrických polárních ekosystémů (CzechPolar2)

Citovat

MANOVÁ, Anna, Blanka HÝŽOVÁ, Diana Darriba CANORA, Ana Castrillo ANTOLIN a Kristýna DUFKOVÁ. Vitality and growth rate of agar plate-cultivated Antarctic microautotrophs: Analysis of PSII functioning by chlorophyll fluorescence parameters. Czech polar reports. Brno: Masaryk University, 2022, roč. 12, č. 2, s. 269-279. ISSN 1805-0689. Dostupné z: https://dx.doi.org/10.5817/CPR2022-2-20.

@article{2267698,
   author = {Manová, Anna and Hýžová, Blanka and Canora, Diana Darriba and Antolin, Ana Castrillo and Dufková, Kristýna},
   article_location = {Brno},
   article_number = {2},
   doi = {http://dx.doi.org/10.5817/CPR2022-2-20},
   keywords = {lichen photobionts; algal biotechnology; Kautsky kinetics},
   language = {eng},
   issn = {1805-0689},
   journal = {Czech polar reports},
   title = {Vitality and growth rate of agar plate-cultivated Antarctic microautotrophs: Analysis of PSII functioning by chlorophyll fluorescence parameters},
   url = {https://journals.muni.cz/CPR/article/view/33535},
   volume = {12},
   year = {2022}
}

TY  - JOUR
ID  - 2267698
AU  - Manová, Anna - Hýžová, Blanka - Canora, Diana Darriba - Antolin, Ana Castrillo - Dufková, Kristýna
PY  - 2022
TI  - Vitality and growth rate of agar plate-cultivated Antarctic microautotrophs: Analysis of PSII functioning by chlorophyll fluorescence parameters
JF  - Czech polar reports
VL  - 12
IS  - 2
SP  - 269-279
EP  - 269-279
PB  - Masaryk University
SN  - 18050689
KW  - lichen photobionts
KW  - algal biotechnology
KW  - Kautsky kinetics
UR  - https://journals.muni.cz/CPR/article/view/33535
N2  - In our study, we focused on the growth of three different microautotrophs isolated from Antarctic lichens (Placopsis contortuplicata, Solorina spongiosa) and cryptoendolithic algal vegetation. The isolates were purified and inoculated on agar plates, Bold ' s Basal Medium (BBM). The growth of the cultures and the markers of physiological (photo-synthetic) activity were monitored by chlorophyll fluorescence in 1 week intervals for 3 months after inoculation. For the assessment of photosynthetic activity, the meth-od of slow Kautsky kinetics supplemented with saturation pulses was applied. Four chlorophyll fluorescence parameters calculated: (1) maximum quantum yield of PSII (FV/FM), (2) effective quantum yield of photosynthetic processes in PSII (phi PSII), (3) non -photochemical quenching of chlorophyll fluorescence, and (4) background chlorophyll fluorescence ratio (F0/F0 '). Troughout the cultivation period, the maximum quantum yield of PSII (FV/FM) showed high values in all three autotrophs with only slight increase in the first part of the cultivation period, followed by slight decrease in the second part. The phi PSII values showed a rapid decline within the first 4 weeks of cultivation followed by more or less constant values in the isolates from P. contortuplicata and cryptoen-dolithic alga. Contrastingly, time course of phi PSII rather showed an increase followed by a decrease in S. spongiosa isolate. NPQ (related to the activation of protective mecha-nisms) increased in the second part of cultivation period, the rate of increase and maxi-mum values were species-specific. The species-specific differences in chlorophyll fluo-rescence parameters are discussed as well as their potential for evaluation of photo-synthetic performance of in vitro cultivated algal/cyanobacterial cultures on agar plates.
ER  -

MANOVÁ, Anna, Blanka HÝŽOVÁ, Diana Darriba CANORA, Ana Castrillo ANTOLIN a Kristýna DUFKOVÁ. Vitality and growth rate of agar plate-cultivated Antarctic microautotrophs: Analysis of PSII functioning by chlorophyll fluorescence parameters. \textit{Czech polar reports}. Brno: Masaryk University, 2022, roč.~12, č.~2, s.~269-279. ISSN~1805-0689. Dostupné z: https://dx.doi.org/10.5817/CPR2022-2-20.

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