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@inbook{2269118,
author = {Drápela, Stanislav and Fedr, Radek and Vacek, Ondřej and Remšík, Ján and Souček, Karel},
address = {New York},
booktitle = {Apoptosis and Cancer : Methods and Protocols},
doi = {http://dx.doi.org/10.1007/978-1-0716-2553-8_9},
editor = {Hugo Barcenilla; David Diaz},
keywords = {Multicolor flow cytometry; Fluorescent cell barcoding; Cell surface phenotyping; High-throughput screening},
howpublished = {tištěná verze "print"},
language = {eng},
location = {New York},
isbn = {978-1-0716-2552-1},
pages = {99-111},
publisher = {Humana Press Inc.},
title = {High-Throughput, Parallel Flow Cytometry Screening of Hundreds of Cell Surface Antigens Using Fluorescent Barcoding},
url = {https://doi.org/10.1007/978-1-0716-2553-8_9},
year = {2022}
}
TY - CHAP
ID - 2269118
AU - Drápela, Stanislav - Fedr, Radek - Vacek, Ondřej - Remšík, Ján - Souček, Karel
PY - 2022
TI - High-Throughput, Parallel Flow Cytometry Screening of Hundreds of Cell Surface Antigens Using Fluorescent Barcoding
VL - Methods in Molecular Biology
PB - Humana Press Inc.
CY - New York
SN - 9781071625521
KW - Multicolor flow cytometry
KW - Fluorescent cell barcoding
KW - Cell surface phenotyping
KW - High-throughput screening
UR - https://doi.org/10.1007/978-1-0716-2553-8_9
N2 - Multicolor flow cytometry allows for analysis of tens of cellular parameters in millions of cells at a single-cell resolution within minutes. The lack of technologies that would facilitate feasible and relatively cheap profiling of such a number of cells with an antibody-based approach led us to the development of a high-throughput cytometry-based platform for surface profiling. We coupled the fluorescent cell barcoding with preexisting, commercially available screening tools to analyze cell surface fingerprint at a large scale. This powerful approach will help to identify novel biomarkers and druggable targets and facilitate the discovery of new concepts in immunology, oncology, and developmental biology.
ER -
DRÁPELA, Stanislav, Radek FEDR, Ondřej VACEK, Ján REMŠÍK and Karel SOUČEK. High-Throughput, Parallel Flow Cytometry Screening of Hundreds of Cell Surface Antigens Using Fluorescent Barcoding. In Hugo Barcenilla; David Diaz. \textit{Apoptosis and Cancer : Methods and Protocols}. New York: Humana Press Inc., 2022, p.~99-111. Methods in Molecular Biology. ISBN~978-1-0716-2552-1. Available from: https://dx.doi.org/10.1007/978-1-0716-2553-8\_{}9.
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