Eudragit® RL coated pellets with immobilized
butyrylcholinesterase for rapid detection of cholinesterase
inhibitors in water

a 2023

Eudragit® RL coated pellets with immobilized butyrylcholinesterase for rapid detection of cholinesterase inhibitors in water

ZEMAN, Jiří, David VETCHÝ, Sylvie PAVLOKOVÁ, Aleš FRANC, Vladimír PITSCHMANN et. al.

Základní údaje

Originální název

Eudragit® RL coated pellets with immobilized butyrylcholinesterase for rapid detection of cholinesterase inhibitors in water

Autoři

ZEMAN, Jiří (203 Česká republika, garant, domácí), David VETCHÝ (203 Česká republika, domácí), Sylvie PAVLOKOVÁ (203 Česká republika, domácí), Aleš FRANC (203 Česká republika, domácí) a Vladimír PITSCHMANN (203 Česká republika)

Vydání

4th European Conference on Pharmaceutics, 2023

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30104 Pharmacology and pharmacy

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Webová stránka konference

Kód RIV

RIV/00216224:14160/23:00134605

Organizační jednotka

Farmaceutická fakulta

Klíčová slova anglicky

detection tube; nerve agent; Neusilin; cholinesterase; color transition; stability

Štítky

ÚFT

Příznaky

Mezinárodní význam

Změněno: 14. 3. 2024 11:01, PharmDr. Jiří Zeman, Ph.D.

Anotace

V originále

Nerve agents (soman, sarin, tabun, VX), organophosphate (parathion, malathion) and carbamate (aldicarb) pesticides are commonly referred to as cholinesterase inhibitors (ChI), which are substances with very high toxicity. A fast, cheap, and sensitive sensor is needed to detect ChI as quickly as possible in order to carry out the appropriate treatment for those affected. Detection tubes (DTs) that use pellets with immobilized cholinesterase for colorimetric detection of the ChI presence, are just the type of detector that meets the above-mentioned requirements. Moreover, enzyme-based biosensors such as DTs have a detection limit between 0.005–0.01 mg∙m−3, which is the lowest in comparison with more expensive and unportable detectors using GC-MS, FTIR or IMS. DTs are based on fast enzymatic Ellman reaction. However, it suffers from an indistinct color transition from white to yellow in the absence of ChI. In this study, an innovative carrier in the form of coated matrix pellets was prepared utilizing the advantages of the previous experiment. The pellet cores contained the unique sorbent – Neusilin® US2 and the semipermeable coating consisted mainly of Eudragit® RL. The main aim of the experiment was to evaluate reaction rate, sensitivity, and color transition intensity of prepared samples in comparison with previous types of carriers.

Návaznosti

VI20192022172, projekt VaV

Název: Detekční trubičky druhé generace pro kontinuální a opakované monitorování nervově paralytických a jiných toxických látek

Investor: Ministerstvo vnitra ČR, Detekční trubičky druhé generace pro kontinuální a opakované monitorování nervově paralytických a jiných toxických látek

Citovat

ZEMAN, Jiří, David VETCHÝ, Sylvie PAVLOKOVÁ, Aleš FRANC a Vladimír PITSCHMANN. Eudragit® RL coated pellets with immobilized butyrylcholinesterase for rapid detection of cholinesterase inhibitors in water. In 4th European Conference on Pharmaceutics. 2023.

@proceedings{2270422,
   author = {Zeman, Jiří and Vetchý, David and Pavloková, Sylvie and Franc, Aleš and Pitschmann, Vladimír},
   booktitle = {4th European Conference on Pharmaceutics},
   keywords = {detection tube; nerve agent; Neusilin; cholinesterase; color transition; stability},
   language = {eng},
   title = {Eudragit® RL coated pellets with immobilized butyrylcholinesterase for rapid detection of cholinesterase inhibitors in water},
   url = {https://www.europeanmeeting.org/home/},
   year = {2023}
}

TY  - CONF
ID  - 2270422
AU  - Zeman, Jiří - Vetchý, David - Pavloková, Sylvie - Franc, Aleš - Pitschmann, Vladimír
PY  - 2023
TI  - Eudragit® RL coated pellets with immobilized butyrylcholinesterase for rapid detection of cholinesterase inhibitors in water
KW  - detection tube
KW  - nerve agent
KW  - Neusilin
KW  - cholinesterase
KW  - color transition
KW  - stability
UR  - https://www.europeanmeeting.org/home/
N2  - Nerve agents (soman, sarin, tabun, VX), organophosphate (parathion, malathion) and carbamate (aldicarb) pesticides are commonly referred to as cholinesterase inhibitors (ChI), which are substances with very high toxicity. A fast, cheap, and sensitive sensor is needed to detect ChI as quickly as possible in order to carry out the appropriate treatment for those affected. Detection tubes (DTs) that use pellets with immobilized cholinesterase for colorimetric detection of the ChI presence, are just the type of detector that meets the above-mentioned requirements. Moreover, enzyme-based biosensors such as DTs have a detection limit between 0.005–0.01 mg∙m−3, which is the lowest in comparison with more expensive and unportable detectors using GC-MS, FTIR or IMS. DTs are based on fast enzymatic Ellman reaction. However, it suffers from an indistinct color transition from white to yellow in the absence of ChI. In this study, an innovative carrier in the form of coated matrix pellets was prepared utilizing the advantages of the previous experiment. The pellet cores contained the unique sorbent – Neusilin® US2 and the semipermeable coating consisted mainly of Eudragit® RL. The main aim of the experiment was to evaluate reaction rate, sensitivity, and color transition intensity of prepared samples in comparison with previous types of carriers.
ER  -

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