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@article{2271653, author = {Mahrík, Lenka and Štefanovie, Barbora and Maresova, Anna and Princova, Jarmila and Kolesár, Peter and Lelkes, Edit and Faux, Celline and Helmlinger, Dominique and Prevorovsky, Martin and Paleček, Jan}, article_location = {LONDON}, article_number = {1}, doi = {http://dx.doi.org/10.1186/s13072-023-00480-z}, keywords = {Genetic and protein-protein interactions; SMC5/6 complex; Nse3 KITE; SAGA histone acetyltransferase module; Gcn5; Ada2; Chromatin accessibility; DNA repair; rDNA; Gene regions}, language = {eng}, issn = {1756-8935}, journal = {EPIGENETICS & CHROMATIN}, title = {The SAGA histone acetyltransferase module targets SMC5/6 to specific genes}, url = {https://epigeneticsandchromatin.biomedcentral.com/articles/10.1186/s13072-023-00480-z}, volume = {16}, year = {2023} }
TY - JOUR ID - 2271653 AU - Mahrík, Lenka - Štefanovie, Barbora - Maresova, Anna - Princova, Jarmila - Kolesár, Peter - Lelkes, Edit - Faux, Celline - Helmlinger, Dominique - Prevorovsky, Martin - Paleček, Jan PY - 2023 TI - The SAGA histone acetyltransferase module targets SMC5/6 to specific genes JF - EPIGENETICS & CHROMATIN VL - 16 IS - 1 SP - 1-16 EP - 1-16 PB - BMC SN - 17568935 KW - Genetic and protein-protein interactions KW - SMC5/6 complex KW - Nse3 KITE KW - SAGA histone acetyltransferase module KW - Gcn5 KW - Ada2 KW - Chromatin accessibility KW - DNA repair KW - rDNA KW - Gene regions UR - https://epigeneticsandchromatin.biomedcentral.com/articles/10.1186/s13072-023-00480-z N2 - Structural Maintenance of Chromosomes (SMC) complexes are molecular machines driving chromatin organization at higher levels. In eukaryotes, three SMC complexes (cohesin, condensin and SMC5/6) play key roles in cohesion, condensation, replication, transcription and DNA repair. Their physical binding to DNA requires accessible chromatin. We performed a genetic screen in fission yeast to identify novel factors required for SMC5/6 binding to DNA. We identified 79 genes of which histone acetyltransferases (HATs) were the most represented. Genetic and phenotypic analyses suggested a particularly strong functional relationship between the SMC5/6 and SAGA complexes. Furthermore, several SMC5/6 subunits physically interacted with SAGA HAT module components Gcn5 and Ada2. As Gcn5-dependent acetylation facilitates the accessibility of chromatin to DNA-repair proteins, we first analysed the formation of DNA-damage-induced SMC5/6 foci in the Delta gcn5 mutant. The SMC5/6 foci formed normally in Delta gcn5, suggesting SAGA-independent SMC5/6 localization to DNA-damaged sites. Next, we used Nse4-FLAG chromatin-immunoprecipitation (ChIP-seq) analysis in unchallenged cells to assess SMC5/6 distribution. A significant portion of SMC5/6 accumulated within gene regions in wild-type cells, which was reduced in Delta gcn5 and Delta ada2 mutants. The drop in SMC5/6 levels was also observed in gcn5-E191Q acetyltransferase-dead mutant. Our data show genetic and physical interactions between SMC5/6 and SAGA complexes. The ChIP-seq analysis suggests that SAGA HAT module targets SMC5/6 to specific gene regions and facilitates their accessibility for SMC5/6 loading. ER -
MAHRÍK, Lenka, Barbora ŠTEFANOVIE, Anna MARESOVA, Jarmila PRINCOVA, Peter KOLESÁR, Edit LELKES, Celline FAUX, Dominique HELMLINGER, Martin PREVOROVSKY a Jan PALEČEK. The SAGA histone acetyltransferase module targets SMC5/6 to specific genes. \textit{EPIGENETICS \&{} CHROMATIN}. LONDON: BMC, 2023, roč.~16, č.~1, s.~1-16. ISSN~1756-8935. Dostupné z: https://dx.doi.org/10.1186/s13072-023-00480-z.
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