The SAGA histone acetyltransferase module targets SMC5/6 to
specific genes

J 2023

The SAGA histone acetyltransferase module targets SMC5/6 to specific genes

MAHRÍK, Lenka, Barbora ŠTEFANOVIE, Anna MARESOVA, Jarmila PRINCOVA, Peter KOLESÁR et. al.

Základní údaje

Originální název

The SAGA histone acetyltransferase module targets SMC5/6 to specific genes

Autoři

MAHRÍK, Lenka (703 Slovensko, domácí), Barbora ŠTEFANOVIE (703 Slovensko, domácí), Anna MARESOVA (203 Česká republika), Jarmila PRINCOVA (203 Česká republika), Peter KOLESÁR (703 Slovensko, domácí), Edit LELKES (703 Slovensko, domácí), Celline FAUX (250 Francie), Dominique HELMLINGER (250 Francie), Martin PREVOROVSKY (203 Česká republika) a Jan PALEČEK (203 Česká republika, garant, domácí)

Vydání

EPIGENETICS & CHROMATIN, LONDON, BMC, 2023, 1756-8935

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.900 v roce 2022

Kód RIV

RIV/00216224:14310/23:00130526

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1186/s13072-023-00480-z

UT WoS

000944569400001

Klíčová slova anglicky

Genetic and protein-protein interactions; SMC5/6 complex; Nse3 KITE; SAGA histone acetyltransferase module; Gcn5; Ada2; Chromatin accessibility; DNA repair; rDNA; Gene regions

Štítky

CF GEN, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 3. 2024 09:23, Mgr. Eva Dubská

Anotace

V originále

Structural Maintenance of Chromosomes (SMC) complexes are molecular machines driving chromatin organization at higher levels. In eukaryotes, three SMC complexes (cohesin, condensin and SMC5/6) play key roles in cohesion, condensation, replication, transcription and DNA repair. Their physical binding to DNA requires accessible chromatin. We performed a genetic screen in fission yeast to identify novel factors required for SMC5/6 binding to DNA. We identified 79 genes of which histone acetyltransferases (HATs) were the most represented. Genetic and phenotypic analyses suggested a particularly strong functional relationship between the SMC5/6 and SAGA complexes. Furthermore, several SMC5/6 subunits physically interacted with SAGA HAT module components Gcn5 and Ada2. As Gcn5-dependent acetylation facilitates the accessibility of chromatin to DNA-repair proteins, we first analysed the formation of DNA-damage-induced SMC5/6 foci in the Delta gcn5 mutant. The SMC5/6 foci formed normally in Delta gcn5, suggesting SAGA-independent SMC5/6 localization to DNA-damaged sites. Next, we used Nse4-FLAG chromatin-immunoprecipitation (ChIP-seq) analysis in unchallenged cells to assess SMC5/6 distribution. A significant portion of SMC5/6 accumulated within gene regions in wild-type cells, which was reduced in Delta gcn5 and Delta ada2 mutants. The drop in SMC5/6 levels was also observed in gcn5-E191Q acetyltransferase-dead mutant. Our data show genetic and physical interactions between SMC5/6 and SAGA complexes. The ChIP-seq analysis suggests that SAGA HAT module targets SMC5/6 to specific gene regions and facilitates their accessibility for SMC5/6 loading.

Návaznosti

LM2018132, projekt VaV

Název: Národní centrum lékařské genomiky (Akronym: NCLG)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní centrum lékařské genomiky

LTC20033, projekt VaV

Název: The SMC5/6 complex in nucleome organization (Akronym: Nukleom)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, The SMC5/6 complex in nucleome organization, INTER-COST

MUNI/R/1142/2021, interní kód MU

Název: Visualization of the 3D structure of chromatin

Investor: Masarykova univerzita, Visualization of the 3D structure of chromatin, CAREER RESTART

MUNI/R/1262/2022, interní kód MU

Název: Vztah mezi opravou DNA a transkripcí

Investor: Masarykova univerzita, Vztah mezi opravou DNA a transkripcí, CAREER RESTART

Citovat

MAHRÍK, Lenka, Barbora ŠTEFANOVIE, Anna MARESOVA, Jarmila PRINCOVA, Peter KOLESÁR, Edit LELKES, Celline FAUX, Dominique HELMLINGER, Martin PREVOROVSKY a Jan PALEČEK. The SAGA histone acetyltransferase module targets SMC5/6 to specific genes. EPIGENETICS & CHROMATIN. LONDON: BMC, 2023, roč. 16, č. 1, s. 1-16. ISSN 1756-8935. Dostupné z: https://dx.doi.org/10.1186/s13072-023-00480-z.

@article{2271653,
   author = {Mahrík, Lenka and Štefanovie, Barbora and Maresova, Anna and Princova, Jarmila and Kolesár, Peter and Lelkes, Edit and Faux, Celline and Helmlinger, Dominique and Prevorovsky, Martin and Paleček, Jan},
   article_location = {LONDON},
   article_number = {1},
   doi = {http://dx.doi.org/10.1186/s13072-023-00480-z},
   keywords = {Genetic and protein-protein interactions; SMC5/6 complex; Nse3 KITE; SAGA histone acetyltransferase module; Gcn5; Ada2; Chromatin accessibility; DNA repair; rDNA; Gene regions},
   language = {eng},
   issn = {1756-8935},
   journal = {EPIGENETICS & CHROMATIN},
   title = {The SAGA histone acetyltransferase module targets SMC5/6 to specific genes},
   url = {https://epigeneticsandchromatin.biomedcentral.com/articles/10.1186/s13072-023-00480-z},
   volume = {16},
   year = {2023}
}

TY  - JOUR
ID  - 2271653
AU  - Mahrík, Lenka - Štefanovie, Barbora - Maresova, Anna - Princova, Jarmila - Kolesár, Peter - Lelkes, Edit - Faux, Celline - Helmlinger, Dominique - Prevorovsky, Martin - Paleček, Jan
PY  - 2023
TI  - The SAGA histone acetyltransferase module targets SMC5/6 to specific genes
JF  - EPIGENETICS & CHROMATIN
VL  - 16
IS  - 1
SP  - 1-16
EP  - 1-16
PB  - BMC
SN  - 17568935
KW  - Genetic and protein-protein interactions
KW  - SMC5/6 complex
KW  - Nse3 KITE
KW  - SAGA histone acetyltransferase module
KW  - Gcn5
KW  - Ada2
KW  - Chromatin accessibility
KW  - DNA repair
KW  - rDNA
KW  - Gene regions
UR  - https://epigeneticsandchromatin.biomedcentral.com/articles/10.1186/s13072-023-00480-z
N2  - Structural Maintenance of Chromosomes (SMC) complexes are molecular machines driving chromatin organization at higher levels. In eukaryotes, three SMC complexes (cohesin, condensin and SMC5/6) play key roles in cohesion, condensation, replication, transcription and DNA repair. Their physical binding to DNA requires accessible chromatin. We performed a genetic screen in fission yeast to identify novel factors required for SMC5/6 binding to DNA. We identified 79 genes of which histone acetyltransferases (HATs) were the most represented. Genetic and phenotypic analyses suggested a particularly strong functional relationship between the SMC5/6 and SAGA complexes. Furthermore, several SMC5/6 subunits physically interacted with SAGA HAT module components Gcn5 and Ada2. As Gcn5-dependent acetylation facilitates the accessibility of chromatin to DNA-repair proteins, we first analysed the formation of DNA-damage-induced SMC5/6 foci in the Delta gcn5 mutant. The SMC5/6 foci formed normally in Delta gcn5, suggesting SAGA-independent SMC5/6 localization to DNA-damaged sites. Next, we used Nse4-FLAG chromatin-immunoprecipitation (ChIP-seq) analysis in unchallenged cells to assess SMC5/6 distribution. A significant portion of SMC5/6 accumulated within gene regions in wild-type cells, which was reduced in Delta gcn5 and Delta ada2 mutants. The drop in SMC5/6 levels was also observed in gcn5-E191Q acetyltransferase-dead mutant. Our data show genetic and physical interactions between SMC5/6 and SAGA complexes. The ChIP-seq analysis suggests that SAGA HAT module targets SMC5/6 to specific gene regions and facilitates their accessibility for SMC5/6 loading.
ER  -

MAHRÍK, Lenka, Barbora ŠTEFANOVIE, Anna MARESOVA, Jarmila PRINCOVA, Peter KOLESÁR, Edit LELKES, Celline FAUX, Dominique HELMLINGER, Martin PREVOROVSKY a Jan PALEČEK. The SAGA histone acetyltransferase module targets SMC5/6 to specific genes. \textit{EPIGENETICS \&{} CHROMATIN}. LONDON: BMC, 2023, roč.~16, č.~1, s.~1-16. ISSN~1756-8935. Dostupné z: https://dx.doi.org/10.1186/s13072-023-00480-z.

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