| KLODOVÁ, Božena, David POTĚŠIL, Lenka STEINBACHOVÁ, Christos MICHAILIDIS, Ann-Cathrin LINDNER, Dieter HACKENBERG, Jorg BECKER, Zbyněk ZDRÁHAL, David TWELL a David HONYS. Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis. PLANT REPRODUCTION. GERMANY: SPRINGER, 2023, roč. 36, č. 3, s. 213-241. ISSN 2194-7953. Dostupné z: https://dx.doi.org/10.1007/s00497-022-00452-5. |
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@article{2276201,
author = {Klodová, Božena and Potěšil, David and Steinbachová, Lenka and Michailidis, Christos and Lindner, AnnandCathrin and Hackenberg, Dieter and Becker, Jorg and Zdráhal, Zbyněk and Twell, David and Honys, David},
article_location = {GERMANY},
article_number = {3},
doi = {http://dx.doi.org/10.1007/s00497-022-00452-5},
keywords = {Arabidopsis; Male gametophyte; RNA-seq; Proteome; Microgametogenesis},
language = {eng},
issn = {2194-7953},
journal = {PLANT REPRODUCTION},
title = {Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis},
url = {https://link.springer.com/article/10.1007/s00497-022-00452-5},
volume = {36},
year = {2023}
}
TY - JOUR
ID - 2276201
AU - Klodová, Božena - Potěšil, David - Steinbachová, Lenka - Michailidis, Christos - Lindner, Ann-Cathrin - Hackenberg, Dieter - Becker, Jorg - Zdráhal, Zbyněk - Twell, David - Honys, David
PY - 2023
TI - Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis
JF - PLANT REPRODUCTION
VL - 36
IS - 3
SP - 213-241
EP - 213-241
PB - SPRINGER
SN - 21947953
KW - Arabidopsis
KW - Male gametophyte
KW - RNA-seq
KW - Proteome
KW - Microgametogenesis
UR - https://link.springer.com/article/10.1007/s00497-022-00452-5
N2 - Sexual reproduction in angiosperms requires the production and delivery of two male gametes by a three-celled haploid male gametophyte. This demands synchronized gene expression in a short developmental window to ensure double fertilization and seed set. While transcriptomic changes in developing pollen are known for Arabidopsis, no studies have integrated RNA and proteomic data in this model. Further, the role of alternative splicing has not been fully addressed, yet post-transcriptional and post-translational regulation may have a key role in gene expression dynamics during microgametogenesis. We have refined and substantially updated global transcriptomic and proteomic changes in developing pollen for two Arabidopsis accessions. Despite the superiority of RNA-seq over microarray-based platforms, we demonstrate high reproducibility and comparability. We identify thousands of long non-coding RNAs as potential regulators of pollen development, hundreds of changes in alternative splicing and provide insight into mRNA translation rate and storage in developing pollen. Our analysis delivers an integrated perspective of gene expression dynamics in developing Arabidopsis pollen and a foundation for studying the role of alternative splicing in this model.
ER -
KLODOVÁ, Božena, David POTĚŠIL, Lenka STEINBACHOVÁ, Christos MICHAILIDIS, Ann-Cathrin LINDNER, Dieter HACKENBERG, Jorg BECKER, Zbyněk ZDRÁHAL, David TWELL a David HONYS. Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis. \textit{PLANT REPRODUCTION}. GERMANY: SPRINGER, 2023, roč.~36, č.~3, s.~213-241. ISSN~2194-7953. Dostupné z: https://dx.doi.org/10.1007/s00497-022-00452-5.
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