Regulatory dynamics of gene expression in the developing male
gametophyte of Arabidopsis

J 2023

Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis

KLODOVÁ, Božena, David POTĚŠIL, Lenka STEINBACHOVÁ, Christos MICHAILIDIS, Ann-Cathrin LINDNER et. al.

Základní údaje

Originální název

Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis

Autoři

KLODOVÁ, Božena (203 Česká republika), David POTĚŠIL (203 Česká republika, domácí), Lenka STEINBACHOVÁ (203 Česká republika), Christos MICHAILIDIS (203 Česká republika), Ann-Cathrin LINDNER (620 Portugalsko), Dieter HACKENBERG (276 Německo), Jorg BECKER (620 Portugalsko), Zbyněk ZDRÁHAL (203 Česká republika, garant, domácí), David TWELL (826 Velká Británie a Severní Irsko) a David HONYS (203 Česká republika)

Vydání

PLANT REPRODUCTION, GERMANY, SPRINGER, 2023, 2194-7953

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

fulltext

Impakt faktor

Impact factor: 3.400 v roce 2022

Kód RIV

RIV/00216224:14740/23:00130612

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1007/s00497-022-00452-5

UT WoS

000871865600001

Klíčová slova anglicky

Arabidopsis; Male gametophyte; RNA-seq; Proteome; Microgametogenesis

Štítky

CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 10. 2024 15:14, Ing. Martina Blahová

Anotace

V originále

Sexual reproduction in angiosperms requires the production and delivery of two male gametes by a three-celled haploid male gametophyte. This demands synchronized gene expression in a short developmental window to ensure double fertilization and seed set. While transcriptomic changes in developing pollen are known for Arabidopsis, no studies have integrated RNA and proteomic data in this model. Further, the role of alternative splicing has not been fully addressed, yet post-transcriptional and post-translational regulation may have a key role in gene expression dynamics during microgametogenesis. We have refined and substantially updated global transcriptomic and proteomic changes in developing pollen for two Arabidopsis accessions. Despite the superiority of RNA-seq over microarray-based platforms, we demonstrate high reproducibility and comparability. We identify thousands of long non-coding RNAs as potential regulators of pollen development, hundreds of changes in alternative splicing and provide insight into mRNA translation rate and storage in developing pollen. Our analysis delivers an integrated perspective of gene expression dynamics in developing Arabidopsis pollen and a foundation for studying the role of alternative splicing in this model.

Návaznosti

EF16_019/0000738, projekt VaV

Název: Centrum experimentální biologie rostlin

EF16_026/0008446, projekt VaV

Název: Integrace signálu a epigenetické reprogramování pro produktivitu rostlin

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LM2018140, projekt VaV

Název: e-Infrastruktura CZ (Akronym: e-INFRA CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, e-Infrastruktura CZ

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

KLODOVÁ, Božena, David POTĚŠIL, Lenka STEINBACHOVÁ, Christos MICHAILIDIS, Ann-Cathrin LINDNER, Dieter HACKENBERG, Jorg BECKER, Zbyněk ZDRÁHAL, David TWELL a David HONYS. Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis. PLANT REPRODUCTION. GERMANY: SPRINGER, 2023, roč. 36, č. 3, s. 213-241. ISSN 2194-7953. Dostupné z: https://dx.doi.org/10.1007/s00497-022-00452-5.

@article{2276201,
   author = {Klodová, Božena and Potěšil, David and Steinbachová, Lenka and Michailidis, Christos and Lindner, AnnandCathrin and Hackenberg, Dieter and Becker, Jorg and Zdráhal, Zbyněk and Twell, David and Honys, David},
   article_location = {GERMANY},
   article_number = {3},
   doi = {http://dx.doi.org/10.1007/s00497-022-00452-5},
   keywords = {Arabidopsis; Male gametophyte; RNA-seq; Proteome; Microgametogenesis},
   language = {eng},
   issn = {2194-7953},
   journal = {PLANT REPRODUCTION},
   title = {Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis},
   url = {https://link.springer.com/article/10.1007/s00497-022-00452-5},
   volume = {36},
   year = {2023}
}

TY  - JOUR
ID  - 2276201
AU  - Klodová, Božena - Potěšil, David - Steinbachová, Lenka - Michailidis, Christos - Lindner, Ann-Cathrin - Hackenberg, Dieter - Becker, Jorg - Zdráhal, Zbyněk - Twell, David - Honys, David
PY  - 2023
TI  - Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis
JF  - PLANT REPRODUCTION
VL  - 36
IS  - 3
SP  - 213-241
EP  - 213-241
PB  - SPRINGER
SN  - 21947953
KW  - Arabidopsis
KW  - Male gametophyte
KW  - RNA-seq
KW  - Proteome
KW  - Microgametogenesis
UR  - https://link.springer.com/article/10.1007/s00497-022-00452-5
N2  - Sexual reproduction in angiosperms requires the production and delivery of two male gametes by a three-celled haploid male gametophyte. This demands synchronized gene expression in a short developmental window to ensure double fertilization and seed set. While transcriptomic changes in developing pollen are known for Arabidopsis, no studies have integrated RNA and proteomic data in this model. Further, the role of alternative splicing has not been fully addressed, yet post-transcriptional and post-translational regulation may have a key role in gene expression dynamics during microgametogenesis. We have refined and substantially updated global transcriptomic and proteomic changes in developing pollen for two Arabidopsis accessions. Despite the superiority of RNA-seq over microarray-based platforms, we demonstrate high reproducibility and comparability. We identify thousands of long non-coding RNAs as potential regulators of pollen development, hundreds of changes in alternative splicing and provide insight into mRNA translation rate and storage in developing pollen. Our analysis delivers an integrated perspective of gene expression dynamics in developing Arabidopsis pollen and a foundation for studying the role of alternative splicing in this model.
ER  -

KLODOVÁ, Božena, David POTĚŠIL, Lenka STEINBACHOVÁ, Christos MICHAILIDIS, Ann-Cathrin LINDNER, Dieter HACKENBERG, Jorg BECKER, Zbyněk ZDRÁHAL, David TWELL a David HONYS. Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis. \textit{PLANT REPRODUCTION}. GERMANY: SPRINGER, 2023, roč.~36, č.~3, s.~213-241. ISSN~2194-7953. Dostupné z: https://dx.doi.org/10.1007/s00497-022-00452-5.

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