Dissolved oxygen saturation is crucial for gas bladder
inflation in turquoise killifish (Nothobranchius furzeri)

J 2023

Dissolved oxygen saturation is crucial for gas bladder inflation in turquoise killifish (Nothobranchius furzeri)

ŽÁK, Jakub, Abhishek Nair ANIL KUMAR NAIR a Iva DYKOVÁ

Základní údaje

Originální název

Dissolved oxygen saturation is crucial for gas bladder inflation in turquoise killifish (Nothobranchius furzeri)

Autoři

ŽÁK, Jakub (203 Česká republika, garant, domácí), Abhishek Nair ANIL KUMAR NAIR (356 Indie, domácí) a Iva DYKOVÁ (203 Česká republika, domácí)

Vydání

Environmental Biology of Fishes, Springer, 2023, 0378-1909

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10605 Developmental biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.400 v roce 2022

Kód RIV

RIV/00216224:14310/23:00130644

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1007/s10641-023-01405-1

UT WoS

000949110800001

Klíčová slova anglicky

Air bladder; Swim bladder; Hatching conditions; Laboratory fish; Sinker syndrome; Belly slider

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 7. 2023 13:30, Mgr. Marie Šípková, DiS.

Anotace

V originále

Providing optimal conditions for early-life gas bladder inflation of captive fish is one of the biggest challenges in fish culture. It also applies to laboratory fishes. Turquoise killifish (Nothobranchius furzeri Jubb, 1971) is a popular research model in biogerontology due to its short lifespan. Annual killifish in laboratory culture frequently suffer from an inability to inflate their gas bladder which may stem from suboptimal environmental conditions in captivity. Here, we investigate (1) the effect of dissolved oxygen (DO) saturation and (2) access to the water surface on gas bladder inflation and hatching success of turquoise killifish. We further histologically examine the gas bladder development from its primordial form to full inflation. In accordance with physoclistous nature of turquoise killifish, access to the water surface is not necessary for gas bladder inflation. We found that hatching success was highest in the treatment with constant or decreasing DO saturation. In contrast, the highest proportion of larvae with inflated gas bladders was found in the treatment with DO oversaturated water (130%) which was induced by the addition of an oxygen tablet. Larvae inflated their gas bladders within 2 to 48 h post-hatching. These findings represent a major step toward a solution to a persistent problem in laboratory culture of this increasingly important model organism.

Citovat

ŽÁK, Jakub, Abhishek Nair ANIL KUMAR NAIR a Iva DYKOVÁ. Dissolved oxygen saturation is crucial for gas bladder inflation in turquoise killifish (Nothobranchius furzeri). Environmental Biology of Fishes. Springer, 2023, roč. 106, č. 4, s. 673-683. ISSN 0378-1909. Dostupné z: https://dx.doi.org/10.1007/s10641-023-01405-1.

@article{2277987,
   author = {Žák, Jakub and Anil Kumar Nair, Abhishek Nair and Dyková, Iva},
   article_number = {4},
   doi = {http://dx.doi.org/10.1007/s10641-023-01405-1},
   keywords = {Air bladder; Swim bladder; Hatching conditions; Laboratory fish; Sinker syndrome; Belly slider},
   language = {eng},
   issn = {0378-1909},
   journal = {Environmental Biology of Fishes},
   title = {Dissolved oxygen saturation is crucial for gas bladder inflation in turquoise killifish (Nothobranchius furzeri)},
   url = {https://link.springer.com/article/10.1007/s10641-023-01405-1},
   volume = {106},
   year = {2023}
}

TY  - JOUR
ID  - 2277987
AU  - Žák, Jakub - Anil Kumar Nair, Abhishek Nair - Dyková, Iva
PY  - 2023
TI  - Dissolved oxygen saturation is crucial for gas bladder inflation in turquoise killifish (Nothobranchius furzeri)
JF  - Environmental Biology of Fishes
VL  - 106
IS  - 4
SP  - 673-683
EP  - 673-683
PB  - Springer
SN  - 03781909
KW  - Air bladder
KW  - Swim bladder
KW  - Hatching conditions
KW  - Laboratory fish
KW  - Sinker syndrome
KW  - Belly slider
UR  - https://link.springer.com/article/10.1007/s10641-023-01405-1
N2  - Providing optimal conditions for early-life gas bladder inflation of captive fish is one of the biggest challenges in fish culture. It also applies to laboratory fishes. Turquoise killifish (Nothobranchius furzeri Jubb, 1971) is a popular research model in biogerontology due to its short lifespan. Annual killifish in laboratory culture frequently suffer from an inability to inflate their gas bladder which may stem from suboptimal environmental conditions in captivity. Here, we investigate (1) the effect of dissolved oxygen (DO) saturation and (2) access to the water surface on gas bladder inflation and hatching success of turquoise killifish. We further histologically examine the gas bladder development from its primordial form to full inflation. In accordance with physoclistous nature of turquoise killifish, access to the water surface is not necessary for gas bladder inflation. We found that hatching success was highest in the treatment with constant or decreasing DO saturation. In contrast, the highest proportion of larvae with inflated gas bladders was found in the treatment with DO oversaturated water (130%) which was induced by the addition of an oxygen tablet. Larvae inflated their gas bladders within 2 to 48 h post-hatching. These findings represent a major step toward a solution to a persistent problem in laboratory culture of this increasingly important model organism.
ER  -

ŽÁK, Jakub, Abhishek Nair ANIL KUMAR NAIR a Iva DYKOVÁ. Dissolved oxygen saturation is crucial for gas bladder inflation in turquoise killifish (Nothobranchius furzeri). \textit{Environmental Biology of Fishes}. Springer, 2023, roč.~106, č.~4, s.~673-683. ISSN~0378-1909. Dostupné z: https://dx.doi.org/10.1007/s10641-023-01405-1.

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