J 2024

Angiotensin II constricts mouse iliac arteries: possible mechanism for aortic aneurysms

GADANEC, Laura Kate, Kristen Renee MCSWEENEY, Peter KUBATKA, Martin CAPRNDA, Ludovit GASPAR et. al.

Basic information

Original name

Angiotensin II constricts mouse iliac arteries: possible mechanism for aortic aneurysms

Authors

GADANEC, Laura Kate, Kristen Renee MCSWEENEY, Peter KUBATKA, Martin CAPRNDA, Ludovit GASPAR, Robert PROSECKÝ (203 Czech Republic, belonging to the institution), Jozef DRAGASEK, Peter KRUŽLIAK (703 Slovakia, guarantor, belonging to the institution), Vasso APOSTOLOPOULOS and Anthony ZULLI

Edition

MOLECULAR AND CELLULAR BIOCHEMISTRY, DORDRECHT, SPRINGER, 2024, 0300-8177

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30201 Cardiac and Cardiovascular systems

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 4.300 in 2022

Organization unit

Faculty of Medicine

DOI

UT WoS

000964127200002

Keywords in English

Abdominal aortic aneurysm; Angiotensin II; Angiotensin type 1 receptor; Angiotensin type 2 receptor; Vasoconstriction

Tags

Tags

International impact, Reviewed
Změněno: 12/7/2024 12:46, Mgr. Tereza Miškechová

Abstract

V originále

Abdominal aortic aneurysms (AAA) result from maladaptive remodeling of the vascular wall and reduces structural integrity. Angiotensin II (AngII) infusion has become a standard laboratory model for studying AAA initiation and progression. We determined the different vasoactive responses of various mouse arteries to Ang II. Ex vivo isometric tension analysis was conducted on 18-week-old male C57BL/6 mice (n = 4) brachiocephalic arteries (BC), iliac arteries (IL), and abdominal (AA) and thoracic aorta (TA). Arterial rings were mounted between organ hooks, gently stretched and an AngII dose response was performed. Rings were placed in 4% paraformaldehyde for immunohistochemistry analysis to quantify peptide expression of angiotensin type 1 (AT(1)R) and 2 receptors (AT(2)R) in the endothelium, media, and adventitia. Results from this study demonstrated vasoconstriction responses in IL were significantly higher at all AngII doses when compared to BC, and TA and AA responses (maximum constriction-IL: 68.64 +/- 5.47% vs. BC: 1.96 +/- 1.00%; TA: 3.13 +/- 0.16% and AA: 2.75 +/- 1.77%, p < 0.0001). Expression of AT(1)R was highest in the endothelium of IL (p < 0.05) and in the media and (p < 0.05) adventitia (p < 0.05) of AA. In contrast, AT(2)R expression was highest in endothelium (p < 0.05), media (p < 0.01, p < 0.05) and adventitia of TA. These results suggest that mouse arteries display different vasoactive responses to AngII, and the exaggerated response in IL arteries may play a role during AAA development.